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The use of RDA (Representational difference analysis) to isolate genomic differences in somaclonal variation of banana.

Somaclonal variation is genetic variation which is observed through the regeneration of cultured somatic cells that usually results in cultured types that was reduced the commercial value during the micropropagation of the valuable elite clones. However, the hypothesis stated that the RDA product be...

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Main Author: Amirah, Hamzah
Format: Undergraduate Final Project Report
Published: 2012
Online Access:http://discol.umk.edu.my/id/eprint/5565/
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spelling my.umk.eprints.55652022-05-23T08:42:04Z http://discol.umk.edu.my/id/eprint/5565/ The use of RDA (Representational difference analysis) to isolate genomic differences in somaclonal variation of banana. Amirah, Hamzah Somaclonal variation is genetic variation which is observed through the regeneration of cultured somatic cells that usually results in cultured types that was reduced the commercial value during the micropropagation of the valuable elite clones. However, the hypothesis stated that the RDA product between normal and somaclonal variation clones of banana contains the genetic variability that can cause different phenotypic variation between the clones. The main purpose of this research is to isolate the unique fragments of DNA between somaclonal and normal banana. The study was conducted by using the nonnal and somaclonal banana of Musa acuminata (Pisang Berangan). Both normal and somaclonal banana had been hybridized and the unique fragment of DNA was isolated. The primers used in this protocol consist of two oligomers, a short strand and a long strand (l2-mer and 24-mer adaptor). RDA successfully isolated few DNA fragments from the normal banana in reaction where the normal banana becomes the tester and the somaclonal banana becomes the driver, but result obtained when vice versa. From the identification, the sequence of genes from the genetic variation can be detennined for the enhancement of the cultured banana. 2012 Undergraduate Final Project Report NonPeerReviewed Amirah, Hamzah (2012) The use of RDA (Representational difference analysis) to isolate genomic differences in somaclonal variation of banana. Undergraduate Final Project Report thesis, Faculty of Agro-Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Somaclonal variation is genetic variation which is observed through the regeneration of cultured somatic cells that usually results in cultured types that was reduced the commercial value during the micropropagation of the valuable elite clones. However, the hypothesis stated that the RDA product between normal and somaclonal variation clones of banana contains the genetic variability that can cause different phenotypic variation between the clones. The main purpose of this research is to isolate the unique fragments of DNA between somaclonal and normal banana. The study was conducted by using the nonnal and somaclonal banana of Musa acuminata (Pisang Berangan). Both normal and somaclonal banana had been hybridized and the unique fragment of DNA was isolated. The primers used in this protocol consist of two oligomers, a short strand and a long strand (l2-mer and 24-mer adaptor). RDA successfully isolated few DNA fragments from the normal banana in reaction where the normal banana becomes the tester and the somaclonal banana becomes the driver, but result obtained when vice versa. From the identification, the sequence of genes from the genetic variation can be detennined for the enhancement of the cultured banana.
format Undergraduate Final Project Report
author Amirah, Hamzah
spellingShingle Amirah, Hamzah
The use of RDA (Representational difference analysis) to isolate genomic differences in somaclonal variation of banana.
author_facet Amirah, Hamzah
author_sort Amirah, Hamzah
title The use of RDA (Representational difference analysis) to isolate genomic differences in somaclonal variation of banana.
title_short The use of RDA (Representational difference analysis) to isolate genomic differences in somaclonal variation of banana.
title_full The use of RDA (Representational difference analysis) to isolate genomic differences in somaclonal variation of banana.
title_fullStr The use of RDA (Representational difference analysis) to isolate genomic differences in somaclonal variation of banana.
title_full_unstemmed The use of RDA (Representational difference analysis) to isolate genomic differences in somaclonal variation of banana.
title_sort use of rda (representational difference analysis) to isolate genomic differences in somaclonal variation of banana.
publishDate 2012
url http://discol.umk.edu.my/id/eprint/5565/
_version_ 1763303571269353472
score 13.211869

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