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Effect of different plant growth regulators on responses of different explants in Callus induction of Java Tea (Orthosiphon stamineus Benth.)

The present study was carried out to identify the most suitable explants of Orthoszphon starnineus Benth. for callus induction to determine the most appropriate concentration of plant growth regulators combination for the callus induction, and to identify the appropriate medium for somatic embryo fo...

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Main Author: Alex Chew Sheng Hsien
Format: Undergraduate Final Project Report
Published: 2012
Online Access:http://discol.umk.edu.my/id/eprint/5564/
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spelling my.umk.eprints.55642022-05-23T08:42:03Z http://discol.umk.edu.my/id/eprint/5564/ Effect of different plant growth regulators on responses of different explants in Callus induction of Java Tea (Orthosiphon stamineus Benth.) Alex Chew Sheng Hsien The present study was carried out to identify the most suitable explants of Orthoszphon starnineus Benth. for callus induction to determine the most appropriate concentration of plant growth regulators combination for the callus induction, and to identify the appropriate medium for somatic embryo formation. Three types of explants (leaf, petiole, and stem nodes) were surface sterilized with the variation of Clorox® concentration for establishment of aseptic culture. Murashige and Skoog (MS) medium with different concentrations of 1-napthalene acetic acid (NAA) and 2,4- dichlorophenoxyacetic acid (2,4-D) were tested to induce the callus. The highest fresh weight callus production was obtained from leaf explants after 4 weeks of culture and 75% of the leaf explants showed callus formation. MS medium supplemented with 0.5 mg 1-1 2,4-D produced 0.42 g of callus fresh weight and was found as the best medium for callus induction from leaf explants. On the other hand, MS medium supplemented with 1.0 mg 1-1 2,4-D + 2.0 ma r1 NAA induced the most calli growth (0.60 g) from leaf explants. The color of the callus produced were creamy white to pale green, greenish, as well as brownish in nature. For study of somatic embryo formation, eight combination concentrations of 6-benzylaminopurine (BAP) and 2,4-D were tested. However, no somatic embryos were induced in all concentrations of BAP (0.0- 1.5 mg 1.1) combined with 0.5 mg 1.1 2,4-D. Further study of somatic embryogenesis of Orthosiphon stamineus Benth. should be carried out to determine the best condition for the production of somatic embryos in this plant. 2012 Undergraduate Final Project Report NonPeerReviewed Alex Chew Sheng Hsien (2012) Effect of different plant growth regulators on responses of different explants in Callus induction of Java Tea (Orthosiphon stamineus Benth.). Undergraduate Final Project Report thesis, Faculty of Agro-Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description The present study was carried out to identify the most suitable explants of Orthoszphon starnineus Benth. for callus induction to determine the most appropriate concentration of plant growth regulators combination for the callus induction, and to identify the appropriate medium for somatic embryo formation. Three types of explants (leaf, petiole, and stem nodes) were surface sterilized with the variation of Clorox® concentration for establishment of aseptic culture. Murashige and Skoog (MS) medium with different concentrations of 1-napthalene acetic acid (NAA) and 2,4- dichlorophenoxyacetic acid (2,4-D) were tested to induce the callus. The highest fresh weight callus production was obtained from leaf explants after 4 weeks of culture and 75% of the leaf explants showed callus formation. MS medium supplemented with 0.5 mg 1-1 2,4-D produced 0.42 g of callus fresh weight and was found as the best medium for callus induction from leaf explants. On the other hand, MS medium supplemented with 1.0 mg 1-1 2,4-D + 2.0 ma r1 NAA induced the most calli growth (0.60 g) from leaf explants. The color of the callus produced were creamy white to pale green, greenish, as well as brownish in nature. For study of somatic embryo formation, eight combination concentrations of 6-benzylaminopurine (BAP) and 2,4-D were tested. However, no somatic embryos were induced in all concentrations of BAP (0.0- 1.5 mg 1.1) combined with 0.5 mg 1.1 2,4-D. Further study of somatic embryogenesis of Orthosiphon stamineus Benth. should be carried out to determine the best condition for the production of somatic embryos in this plant.
format Undergraduate Final Project Report
author Alex Chew Sheng Hsien
spellingShingle Alex Chew Sheng Hsien
Effect of different plant growth regulators on responses of different explants in Callus induction of Java Tea (Orthosiphon stamineus Benth.)
author_facet Alex Chew Sheng Hsien
author_sort Alex Chew Sheng Hsien
title Effect of different plant growth regulators on responses of different explants in Callus induction of Java Tea (Orthosiphon stamineus Benth.)
title_short Effect of different plant growth regulators on responses of different explants in Callus induction of Java Tea (Orthosiphon stamineus Benth.)
title_full Effect of different plant growth regulators on responses of different explants in Callus induction of Java Tea (Orthosiphon stamineus Benth.)
title_fullStr Effect of different plant growth regulators on responses of different explants in Callus induction of Java Tea (Orthosiphon stamineus Benth.)
title_full_unstemmed Effect of different plant growth regulators on responses of different explants in Callus induction of Java Tea (Orthosiphon stamineus Benth.)
title_sort effect of different plant growth regulators on responses of different explants in callus induction of java tea (orthosiphon stamineus benth.)
publishDate 2012
url http://discol.umk.edu.my/id/eprint/5564/
_version_ 1763303571136184320
score 13.223943

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