Cover Image

Bacterial diversity of Elephant Dung using 16S rRNA gene sequence analysis

The objective of this study was to identify the bacterial diversity of dung from Asian elephant (Elephas maximus). The important uses of elephant dung have been identified through various studies, but the knowledge for the bacterial diversity of elephant dung is very limited and insufficient. In thi...

Full description

Saved in:
Bibliographic Details
Main Author: Tan Shan Wen
Format: Undergraduate Final Project Report
Language:English
Published: 2019
Online Access:http://discol.umk.edu.my/id/eprint/4844/1/Tan%20Shan%20Wen.pdf
http://discol.umk.edu.my/id/eprint/4844/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.umk.eprints.4844
record_format eprints
spelling my.umk.eprints.48442022-05-23T19:17:20Z http://discol.umk.edu.my/id/eprint/4844/ Bacterial diversity of Elephant Dung using 16S rRNA gene sequence analysis Tan Shan Wen The objective of this study was to identify the bacterial diversity of dung from Asian elephant (Elephas maximus). The important uses of elephant dung have been identified through various studies, but the knowledge for the bacterial diversity of elephant dung is very limited and insufficient. In this study, 16S rRNA gene sequence was used to identify the bacterial distribution and diversity of elephant dung. Thirteen pure bacterial isolates labelled as ED1, ED2, ED3, ED4, ED5, ED6, ED7, ED8, ED9, ED10, ED11, ED12, and ED13 were obtained and basic characterisation of bacteria by Gram staining was performed. Polymerase Chain Reaction (PCR) of the bacteria isolates was done by targeting 16S rRNA gene. Unpurified Polymerase Chain Reaction (PCR) products of ED2, ED5 and ED7 were sent for purification and sequencing. The obtained sequences were compared with the sequences available in GenBank database. BLAST was used to perform identification of bacteria based on the percentage of similarity. Phylogenetic analysis was done by constructing a phylogenetic tree by using Neighbor-Joining (NJ) method. The obtained sequences were BLAST and the results of their phylogenetic analysis showed that ED2 has 90% similarity with Exiguobacterium profundum, ED5 has 98% similarity with Flavobacterium lutescens, and ED7 has 99% similarity with Micrococcus luteus. Micrococcus luteus is cellulolytic bacteria which can produce cellulose enzyme to degrade the cellulose found in plant cell wall. The results obtained from this study provided a preliminary result and further studies of elephant dung is needed to be done for more understanding of microbial diversity of elephant dung. The findings of this study focus on determining the ecological significance of bacteria found in elephant dung. 2019 Undergraduate Final Project Report NonPeerReviewed text en http://discol.umk.edu.my/id/eprint/4844/1/Tan%20Shan%20Wen.pdf Tan Shan Wen (2019) Bacterial diversity of Elephant Dung using 16S rRNA gene sequence analysis. Final Year Project thesis, Universiti Malaysia Kelantan. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
language English
description The objective of this study was to identify the bacterial diversity of dung from Asian elephant (Elephas maximus). The important uses of elephant dung have been identified through various studies, but the knowledge for the bacterial diversity of elephant dung is very limited and insufficient. In this study, 16S rRNA gene sequence was used to identify the bacterial distribution and diversity of elephant dung. Thirteen pure bacterial isolates labelled as ED1, ED2, ED3, ED4, ED5, ED6, ED7, ED8, ED9, ED10, ED11, ED12, and ED13 were obtained and basic characterisation of bacteria by Gram staining was performed. Polymerase Chain Reaction (PCR) of the bacteria isolates was done by targeting 16S rRNA gene. Unpurified Polymerase Chain Reaction (PCR) products of ED2, ED5 and ED7 were sent for purification and sequencing. The obtained sequences were compared with the sequences available in GenBank database. BLAST was used to perform identification of bacteria based on the percentage of similarity. Phylogenetic analysis was done by constructing a phylogenetic tree by using Neighbor-Joining (NJ) method. The obtained sequences were BLAST and the results of their phylogenetic analysis showed that ED2 has 90% similarity with Exiguobacterium profundum, ED5 has 98% similarity with Flavobacterium lutescens, and ED7 has 99% similarity with Micrococcus luteus. Micrococcus luteus is cellulolytic bacteria which can produce cellulose enzyme to degrade the cellulose found in plant cell wall. The results obtained from this study provided a preliminary result and further studies of elephant dung is needed to be done for more understanding of microbial diversity of elephant dung. The findings of this study focus on determining the ecological significance of bacteria found in elephant dung.
format Undergraduate Final Project Report
author Tan Shan Wen
spellingShingle Tan Shan Wen
Bacterial diversity of Elephant Dung using 16S rRNA gene sequence analysis
author_facet Tan Shan Wen
author_sort Tan Shan Wen
title Bacterial diversity of Elephant Dung using 16S rRNA gene sequence analysis
title_short Bacterial diversity of Elephant Dung using 16S rRNA gene sequence analysis
title_full Bacterial diversity of Elephant Dung using 16S rRNA gene sequence analysis
title_fullStr Bacterial diversity of Elephant Dung using 16S rRNA gene sequence analysis
title_full_unstemmed Bacterial diversity of Elephant Dung using 16S rRNA gene sequence analysis
title_sort bacterial diversity of elephant dung using 16s rrna gene sequence analysis
publishDate 2019
url http://discol.umk.edu.my/id/eprint/4844/1/Tan%20Shan%20Wen.pdf
http://discol.umk.edu.my/id/eprint/4844/
_version_ 1763303470899658752
score 13.211869

Similar Items