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Optimisation of proteus mirabilis strain PI18 β-Glucan extraction

Biopolymer, Microbial Polysaccharide (EPS) has showed promising benefit in various industries throughout last decade of study. However, the novelty and the complexity of the microbial EPS lead to drawback in exploiting this area. Previously, PI18 strain has shown promising β-glucan production throug...

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Main Author: Lee, Chun Eng
Format: Undergraduate Final Project Report
Language:English
Published: 2019
Online Access:http://discol.umk.edu.my/id/eprint/4534/1/LEE%20CHUN%20ENG.pdf
http://discol.umk.edu.my/id/eprint/4534/
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spelling my.umk.eprints.45342022-05-23T21:48:09Z http://discol.umk.edu.my/id/eprint/4534/ Optimisation of proteus mirabilis strain PI18 β-Glucan extraction Lee, Chun Eng Biopolymer, Microbial Polysaccharide (EPS) has showed promising benefit in various industries throughout last decade of study. However, the novelty and the complexity of the microbial EPS lead to drawback in exploiting this area. Previously, PI18 strain has shown promising β-glucan production through Scleroglucan base extraction method. In order to optimise further the β-glucan productivity, understanding the biosynthesis of βglucan from PI18 strain by subject the bacteria in nutrient stress and modifying previous extraction strategy to be time and cost efficient. This study has found that PI18 can biosynthesis β-glucan effectively in Minimal medium (Production media) and pH 7.00 in controlled environment (Bioreactor). Current study has achieved two days peak β-glucan production on Day 2-3 (1.412-1.424 mg/mL) by cultivation fresh seed culture. As compare with previous study only show peak β-glucan on Day 4 (2.752 mg/mL). Through modification of Scleroglucan base extraction strategy, the homogenization and three times dilution step can be eliminated to one-time dilution with distilled water can achieved better yield in shorter time. 2019 Undergraduate Final Project Report NonPeerReviewed text en http://discol.umk.edu.my/id/eprint/4534/1/LEE%20CHUN%20ENG.pdf Lee, Chun Eng (2019) Optimisation of proteus mirabilis strain PI18 β-Glucan extraction. Final Year Project thesis, Universiti Malaysia Kelantan. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
language English
description Biopolymer, Microbial Polysaccharide (EPS) has showed promising benefit in various industries throughout last decade of study. However, the novelty and the complexity of the microbial EPS lead to drawback in exploiting this area. Previously, PI18 strain has shown promising β-glucan production through Scleroglucan base extraction method. In order to optimise further the β-glucan productivity, understanding the biosynthesis of βglucan from PI18 strain by subject the bacteria in nutrient stress and modifying previous extraction strategy to be time and cost efficient. This study has found that PI18 can biosynthesis β-glucan effectively in Minimal medium (Production media) and pH 7.00 in controlled environment (Bioreactor). Current study has achieved two days peak β-glucan production on Day 2-3 (1.412-1.424 mg/mL) by cultivation fresh seed culture. As compare with previous study only show peak β-glucan on Day 4 (2.752 mg/mL). Through modification of Scleroglucan base extraction strategy, the homogenization and three times dilution step can be eliminated to one-time dilution with distilled water can achieved better yield in shorter time.
format Undergraduate Final Project Report
author Lee, Chun Eng
spellingShingle Lee, Chun Eng
Optimisation of proteus mirabilis strain PI18 β-Glucan extraction
author_facet Lee, Chun Eng
author_sort Lee, Chun Eng
title Optimisation of proteus mirabilis strain PI18 β-Glucan extraction
title_short Optimisation of proteus mirabilis strain PI18 β-Glucan extraction
title_full Optimisation of proteus mirabilis strain PI18 β-Glucan extraction
title_fullStr Optimisation of proteus mirabilis strain PI18 β-Glucan extraction
title_full_unstemmed Optimisation of proteus mirabilis strain PI18 β-Glucan extraction
title_sort optimisation of proteus mirabilis strain pi18 β-glucan extraction
publishDate 2019
url http://discol.umk.edu.my/id/eprint/4534/1/LEE%20CHUN%20ENG.pdf
http://discol.umk.edu.my/id/eprint/4534/
_version_ 1763303427566206976
score 13.211869

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