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Optimization of DNA extraction of enriched iron-reducing bacteria from tropical freshwater stream sediment for metagenomic analysis

Iron reducing bacteria (IRB) promote the reduction of iron oxides in anoxic soils and sediments and play a major role in the cycling of these metals and other elements such as organic carbon in aqueous environments. Previous studies investigating the IRB focused on cultivable microbial composition....

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Main Author: Mohd Annual, Nur Nadhirah
Format: Undergraduate Final Project Report
Published: 2020
Online Access:http://discol.umk.edu.my/id/eprint/4238/
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spelling my.umk.eprints.42382022-05-23T08:24:02Z http://discol.umk.edu.my/id/eprint/4238/ Optimization of DNA extraction of enriched iron-reducing bacteria from tropical freshwater stream sediment for metagenomic analysis Mohd Annual, Nur Nadhirah Iron reducing bacteria (IRB) promote the reduction of iron oxides in anoxic soils and sediments and play a major role in the cycling of these metals and other elements such as organic carbon in aqueous environments. Previous studies investigating the IRB focused on cultivable microbial composition. The composition of IRB can be varied greatly in the environment and some of them cannot be detected by the conventional method. Thus, there were limited researches about IRB distribution in Malaysia tropical river environment. The DNA from enrichment was extracted for metagenomic analysis. Enrichment incubations were conducted by using different concentration of electron donors. Then, the Fe (II) reduction concentration on hydrous ferrous oxide (HFO) was investigated. In general, higher concentrations of electron donors resulted the high production of Fe2+. The survival of IRB in different concentration of Fe (II) was believed to be associated in determining the microbial composition and microbial shift in the enrichment and environment during reduction process. 2020 Undergraduate Final Project Report NonPeerReviewed Mohd Annual, Nur Nadhirah (2020) Optimization of DNA extraction of enriched iron-reducing bacteria from tropical freshwater stream sediment for metagenomic analysis. Final Year Project thesis, Universiti Malaysia Kelantan. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Iron reducing bacteria (IRB) promote the reduction of iron oxides in anoxic soils and sediments and play a major role in the cycling of these metals and other elements such as organic carbon in aqueous environments. Previous studies investigating the IRB focused on cultivable microbial composition. The composition of IRB can be varied greatly in the environment and some of them cannot be detected by the conventional method. Thus, there were limited researches about IRB distribution in Malaysia tropical river environment. The DNA from enrichment was extracted for metagenomic analysis. Enrichment incubations were conducted by using different concentration of electron donors. Then, the Fe (II) reduction concentration on hydrous ferrous oxide (HFO) was investigated. In general, higher concentrations of electron donors resulted the high production of Fe2+. The survival of IRB in different concentration of Fe (II) was believed to be associated in determining the microbial composition and microbial shift in the enrichment and environment during reduction process.
format Undergraduate Final Project Report
author Mohd Annual, Nur Nadhirah
spellingShingle Mohd Annual, Nur Nadhirah
Optimization of DNA extraction of enriched iron-reducing bacteria from tropical freshwater stream sediment for metagenomic analysis
author_facet Mohd Annual, Nur Nadhirah
author_sort Mohd Annual, Nur Nadhirah
title Optimization of DNA extraction of enriched iron-reducing bacteria from tropical freshwater stream sediment for metagenomic analysis
title_short Optimization of DNA extraction of enriched iron-reducing bacteria from tropical freshwater stream sediment for metagenomic analysis
title_full Optimization of DNA extraction of enriched iron-reducing bacteria from tropical freshwater stream sediment for metagenomic analysis
title_fullStr Optimization of DNA extraction of enriched iron-reducing bacteria from tropical freshwater stream sediment for metagenomic analysis
title_full_unstemmed Optimization of DNA extraction of enriched iron-reducing bacteria from tropical freshwater stream sediment for metagenomic analysis
title_sort optimization of dna extraction of enriched iron-reducing bacteria from tropical freshwater stream sediment for metagenomic analysis
publishDate 2020
url http://discol.umk.edu.my/id/eprint/4238/
_version_ 1763303386000654336
score 13.211869

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