Cover Image

Optimization of protease-treated Black Soldier Fly Larvae (BSFL) using Response Surface Methodology (RSM) for broiler feed

Insects have been recognized as a potential source of protein for both human food and animal feed. Hermelia illucens or commonly known as black soldier fly larvae (BSFL) are a type of insect that can feed and develop on a wide range of feed sources such as on kitchen waste, dairy manure, chicken man...

Full description

Saved in:
Bibliographic Details
Main Author: Mohamad Kamal, Siti Sarah
Format: Undergraduate Final Project Report
Published: 2020
Online Access:http://discol.umk.edu.my/id/eprint/4222/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.umk.eprints.4222
record_format eprints
spelling my.umk.eprints.42222022-05-23T08:23:56Z http://discol.umk.edu.my/id/eprint/4222/ Optimization of protease-treated Black Soldier Fly Larvae (BSFL) using Response Surface Methodology (RSM) for broiler feed Mohamad Kamal, Siti Sarah Insects have been recognized as a potential source of protein for both human food and animal feed. Hermelia illucens or commonly known as black soldier fly larvae (BSFL) are a type of insect that can feed and develop on a wide range of feed sources such as on kitchen waste, dairy manure, chicken manure as well as human feces. This insect has been used as an alternative protein source for a lot of animal feeding including fisheries and poultry. However, the digestive system of animals is not fully efficient. Thus, animals including broiler usually would not be able to fully digest all sources of nutrient upon consuming the feed. Most of the time, nutrient especially protein is being wasted through the animal's manure. Therefore, it is important to find an effective way to improve protein absorption of BSFL so that broiler will be able to completely digest all of the nutrients from the feed formulation. In order to overcome this problem, protease was produced and used to treat the BSFL so that protein in the BSFL can be converted into amino acids, thus improving broiler's nutrient absorption. In this study, the optimum condition for obtaining the highest decrease in protein content (%) of the BSFL was being investigated by using Response Surface Methodology (RSM). A model of Central Composite Design (CCD) with three variables: volume of water (5-8 mL), volume of protease (100-300 µL) and time of incubation (30-90 min) were used to study the response variable which was the decrease in protein content (%). From this study, the protein content of the BSFL before and after the treatment was able to be identified and compared by performing protein analysis. The data analysis generated by Design Expert Software version 11 showed that the model is significant at p < 0.05 which affected the response variable. The effect of the variables and parameters was studied by using a 2D counter plot and 3D response surface graph. The software suggested that the optimum conditions were 5 mL of water, 100 of protease and 30 minutes of incubation time for treating the BSFL in order to obtain 10.5266% decrease in the protein content. 2020 Undergraduate Final Project Report NonPeerReviewed Mohamad Kamal, Siti Sarah (2020) Optimization of protease-treated Black Soldier Fly Larvae (BSFL) using Response Surface Methodology (RSM) for broiler feed. Final Year Project thesis, Universiti Malaysia Kelantan. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Insects have been recognized as a potential source of protein for both human food and animal feed. Hermelia illucens or commonly known as black soldier fly larvae (BSFL) are a type of insect that can feed and develop on a wide range of feed sources such as on kitchen waste, dairy manure, chicken manure as well as human feces. This insect has been used as an alternative protein source for a lot of animal feeding including fisheries and poultry. However, the digestive system of animals is not fully efficient. Thus, animals including broiler usually would not be able to fully digest all sources of nutrient upon consuming the feed. Most of the time, nutrient especially protein is being wasted through the animal's manure. Therefore, it is important to find an effective way to improve protein absorption of BSFL so that broiler will be able to completely digest all of the nutrients from the feed formulation. In order to overcome this problem, protease was produced and used to treat the BSFL so that protein in the BSFL can be converted into amino acids, thus improving broiler's nutrient absorption. In this study, the optimum condition for obtaining the highest decrease in protein content (%) of the BSFL was being investigated by using Response Surface Methodology (RSM). A model of Central Composite Design (CCD) with three variables: volume of water (5-8 mL), volume of protease (100-300 µL) and time of incubation (30-90 min) were used to study the response variable which was the decrease in protein content (%). From this study, the protein content of the BSFL before and after the treatment was able to be identified and compared by performing protein analysis. The data analysis generated by Design Expert Software version 11 showed that the model is significant at p < 0.05 which affected the response variable. The effect of the variables and parameters was studied by using a 2D counter plot and 3D response surface graph. The software suggested that the optimum conditions were 5 mL of water, 100 of protease and 30 minutes of incubation time for treating the BSFL in order to obtain 10.5266% decrease in the protein content.
format Undergraduate Final Project Report
author Mohamad Kamal, Siti Sarah
spellingShingle Mohamad Kamal, Siti Sarah
Optimization of protease-treated Black Soldier Fly Larvae (BSFL) using Response Surface Methodology (RSM) for broiler feed
author_facet Mohamad Kamal, Siti Sarah
author_sort Mohamad Kamal, Siti Sarah
title Optimization of protease-treated Black Soldier Fly Larvae (BSFL) using Response Surface Methodology (RSM) for broiler feed
title_short Optimization of protease-treated Black Soldier Fly Larvae (BSFL) using Response Surface Methodology (RSM) for broiler feed
title_full Optimization of protease-treated Black Soldier Fly Larvae (BSFL) using Response Surface Methodology (RSM) for broiler feed
title_fullStr Optimization of protease-treated Black Soldier Fly Larvae (BSFL) using Response Surface Methodology (RSM) for broiler feed
title_full_unstemmed Optimization of protease-treated Black Soldier Fly Larvae (BSFL) using Response Surface Methodology (RSM) for broiler feed
title_sort optimization of protease-treated black soldier fly larvae (bsfl) using response surface methodology (rsm) for broiler feed
publishDate 2020
url http://discol.umk.edu.my/id/eprint/4222/
_version_ 1763303383683301376
score 13.211869

Similar Items