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Characterization of respiratory capabilities of Shewanella Oneidensis MR-1 Fe (III) reduction deficient mutants

Shewanella oneidensis MR-1 is a free-living gram-negative gamma-proteobacterium with high respiratory versatility which able to respire on a wide range of electron acceptors including insoluble metal oxides. The molecular mechanism on how S.oneidensis MR-1 reduces insoluble metal oxides is still not...

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Main Author: Liu, Dick Hong
Format: Undergraduate Final Project Report
Published: 2020
Online Access:http://discol.umk.edu.my/id/eprint/4193/
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spelling my.umk.eprints.41932022-05-23T08:23:41Z http://discol.umk.edu.my/id/eprint/4193/ Characterization of respiratory capabilities of Shewanella Oneidensis MR-1 Fe (III) reduction deficient mutants Liu, Dick Hong Shewanella oneidensis MR-1 is a free-living gram-negative gamma-proteobacterium with high respiratory versatility which able to respire on a wide range of electron acceptors including insoluble metal oxides. The molecular mechanism on how S.oneidensis MR-1 reduces insoluble metal oxides is still not fully understood and currently under intensive studies. To further elucidate the molecular mechanism on how S.oneidensis MR-1 reduces metal oxides, we recently conducted mutagenesis on S.oneidensis MR-1 wild type strain to generate iron reduction deficient mutants. From the mutagenesis, we successfully isolated two iron reduction-deficient mutants. In this study, the respiratory capabilities of the mutants were further investigated. The mutants were tested for their ability to respire on both soluble and insoluble iron as the terminal electron acceptors. The result of anaerobic growth tests demonstrated that these two strains have remarkable lower Fe (III) reduction capabilities compared to wild-type. Determination of the mutants' respiratory phenotypes and future genetic analyses of the mutated genes in the mutants will provide more insight for a better understanding of the molecular mechanism of MR-1 iron-reduction. 2020 Undergraduate Final Project Report NonPeerReviewed Liu, Dick Hong (2020) Characterization of respiratory capabilities of Shewanella Oneidensis MR-1 Fe (III) reduction deficient mutants. Final Year Project thesis, Universiti Malaysia Kelantan. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Shewanella oneidensis MR-1 is a free-living gram-negative gamma-proteobacterium with high respiratory versatility which able to respire on a wide range of electron acceptors including insoluble metal oxides. The molecular mechanism on how S.oneidensis MR-1 reduces insoluble metal oxides is still not fully understood and currently under intensive studies. To further elucidate the molecular mechanism on how S.oneidensis MR-1 reduces metal oxides, we recently conducted mutagenesis on S.oneidensis MR-1 wild type strain to generate iron reduction deficient mutants. From the mutagenesis, we successfully isolated two iron reduction-deficient mutants. In this study, the respiratory capabilities of the mutants were further investigated. The mutants were tested for their ability to respire on both soluble and insoluble iron as the terminal electron acceptors. The result of anaerobic growth tests demonstrated that these two strains have remarkable lower Fe (III) reduction capabilities compared to wild-type. Determination of the mutants' respiratory phenotypes and future genetic analyses of the mutated genes in the mutants will provide more insight for a better understanding of the molecular mechanism of MR-1 iron-reduction.
format Undergraduate Final Project Report
author Liu, Dick Hong
spellingShingle Liu, Dick Hong
Characterization of respiratory capabilities of Shewanella Oneidensis MR-1 Fe (III) reduction deficient mutants
author_facet Liu, Dick Hong
author_sort Liu, Dick Hong
title Characterization of respiratory capabilities of Shewanella Oneidensis MR-1 Fe (III) reduction deficient mutants
title_short Characterization of respiratory capabilities of Shewanella Oneidensis MR-1 Fe (III) reduction deficient mutants
title_full Characterization of respiratory capabilities of Shewanella Oneidensis MR-1 Fe (III) reduction deficient mutants
title_fullStr Characterization of respiratory capabilities of Shewanella Oneidensis MR-1 Fe (III) reduction deficient mutants
title_full_unstemmed Characterization of respiratory capabilities of Shewanella Oneidensis MR-1 Fe (III) reduction deficient mutants
title_sort characterization of respiratory capabilities of shewanella oneidensis mr-1 fe (iii) reduction deficient mutants
publishDate 2020
url http://discol.umk.edu.my/id/eprint/4193/
_version_ 1763303379562397696
score 13.211869

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