Detection Of Virulence Genes In Aeromonas Hydrophila Isolated From Hybrid Tilapia, Oreochromis Spp.
Aeromonas hydrophila, a pathogenic Gram-negative bacterium, poses significant risks to both aquatic animals and human health. It is well-documented for its role in causing a range of infections, from mild gastroenteritis in humans to severe systemic diseases in fish. Given its prevalence in freshwat...
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| Format: | Undergraduate Final Project Report |
| Language: | English |
| Published: |
2024
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| Online Access: | http://discol.umk.edu.my/id/eprint/14864/1/KOMATESWARY%20A_P%20RARVI%20D19B0010%20.pdf http://discol.umk.edu.my/id/eprint/14864/ |
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| Summary: | Aeromonas hydrophila, a pathogenic Gram-negative bacterium, poses significant risks to both aquatic animals and human health. It is well-documented for its role in causing a range of infections, from mild gastroenteritis in humans to severe systemic diseases in fish. Given its prevalence in freshwater ecosystems, understanding its pathogenicity is vital for developing effective management strategies in aquaculture and mitigating potential public health threats.
This study aimed to identify virulence genes present in various Aeromonas hydrophila samples isolated from hybrid tilapia. The samples analyzed included K3T11(1), K310, K3T8, K1T2(1), and K2T6(b). This selection highlights the diverse ecological niche that Aeromonas hydrophila occupies and its capacity to adapt to different host environments. To achieve this objective, Polymerase Chain Reaction (PCR) was employed as a molecular technique to detect specific virulence genes associated with the pathogenicity of Aeromonas hydrophila. The targeted virulence genes included Haemolysin (hlyA), Aerolysin (aerA), Cytolytic Enterotoxin (act), AscV, and AopB. These genes are crucial as they contribute to the bacterium's ability to cause disease by damaging host tissues, evading immune responses, and facilitating the establishment of infection. The results of the PCR analysis were particularly revealing. Among the samples tested, only the K2T6(b) sample showed a positive result for the Cytolytic Enterotoxin gene (act). This finding is significant, as it indicates the presence of a key virulence factor that may enhance the pathogenic potential of the bacterium in that particular sample. The exclusive identification of this gene in the K2T6(b) sample suggests variability in virulence gene distribution among different Aeromonas hydrophila isolates, which could influence their respective pathogenicity. These findings underscore the critical importance of monitoring virulence factors in Aeromonas hydrophila, as they have far-reaching implications for both aquaculture practices and public health initiatives. By identifying and understanding these virulence genes, we can better assess the risks posed by this pathogen in aquatic systems and develop targeted strategies for disease prevention and control in both fish populations and potentially affected human communities. The research contributes valuable insights to the field of veterinary medicine and highlights the need for ongoing surveillance and research into aquatic pathogens.
Keywords: Aeromonas hydrophila, virulence genes, polymerase chain reaction, sequencing, |
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