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Serological Detection Of Epizootic Hemorrhagic Disease Virus (Ehdv) And Associated Risk Factors In Goats In Kelantan, Malaysia.

Epizootic hemorrhagic disease virus (EHDV) is a notifiable, infectious but non-contagious hemorrhagic disease caused by a non-enveloped double-stranded RNA virus that belongs to the Reoviridae family (genus: Orbivirus). There are 7 known serotypes (EHDV- 1, 2, 4, 5, 6, 7 and 8). This virus is closel...

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Main Author: Maria Fazira Mohd Zulkefli
Format: Undergraduate Final Project Report
Language:English
Published: 2025
Online Access:http://discol.umk.edu.my/id/eprint/14861/1/MARIA%20FAZIRA%20MOHD%20ZULKEFLI%20D20B0091.pdf
http://discol.umk.edu.my/id/eprint/14861/
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spelling my.umk.eprints.148612025-03-02T06:52:56Z http://discol.umk.edu.my/id/eprint/14861/ Serological Detection Of Epizootic Hemorrhagic Disease Virus (Ehdv) And Associated Risk Factors In Goats In Kelantan, Malaysia. Maria Fazira Mohd Zulkefli Epizootic hemorrhagic disease virus (EHDV) is a notifiable, infectious but non-contagious hemorrhagic disease caused by a non-enveloped double-stranded RNA virus that belongs to the Reoviridae family (genus: Orbivirus). There are 7 known serotypes (EHDV- 1, 2, 4, 5, 6, 7 and 8). This virus is closely related to the Bluetongue virus, exhibiting similar clinical presentations and sharing the same transmission vectors. Nonetheless, these two viruses are genetically distinct. The common clinical signs that may be manifested by infected animals are facial oedema, hyperemia of the mucous membrane, excessive salivation, bloody diarrhea, ulceration in the oral cavity and others. Globally, the most reported and widely studied animal related to EHDV is the White-tailed deer as it is the most susceptible animal to EHDV while other species such as cattle may also be infected with less severe clinical signs. However, there are limited studies of EHDV in Malaysia, especially in goats. Therefore, the main objective of this study is to serve as a baseline study for the serological detection of EHDV in goats in Malaysia. For this study, competitive ELISA technique is used to detect the antibody against EHDV VP7 protein. This method is WOAH’s current preferred diagnostic method as it does not cause cross -reactivity between EHDV and Bluetongue virus. In this study, blood samples from 2 farms (total: 40 goats) were collected for the detection of the antibodies in which 3 out of 40 goats were EHDV positive. EHDV is one of the economically important diseases in ruminants as it could lead to economic losses and reduced productivity among infected animals. Therefore, this study can be set as a preliminary study of EHDV status in goats especially in Malaysia for future study. In addition to that, early detection allows faster intervention to control this disease. Keywords: EHDV, WOAH, c-ELISA, VP7 protein 2025-02-05 Undergraduate Final Project Report NonPeerReviewed text en http://discol.umk.edu.my/id/eprint/14861/1/MARIA%20FAZIRA%20MOHD%20ZULKEFLI%20D20B0091.pdf Maria Fazira Mohd Zulkefli (2025) Serological Detection Of Epizootic Hemorrhagic Disease Virus (Ehdv) And Associated Risk Factors In Goats In Kelantan, Malaysia. Final Year Project thesis, Universiti Malaysia Kelantan. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
language English
description Epizootic hemorrhagic disease virus (EHDV) is a notifiable, infectious but non-contagious hemorrhagic disease caused by a non-enveloped double-stranded RNA virus that belongs to the Reoviridae family (genus: Orbivirus). There are 7 known serotypes (EHDV- 1, 2, 4, 5, 6, 7 and 8). This virus is closely related to the Bluetongue virus, exhibiting similar clinical presentations and sharing the same transmission vectors. Nonetheless, these two viruses are genetically distinct. The common clinical signs that may be manifested by infected animals are facial oedema, hyperemia of the mucous membrane, excessive salivation, bloody diarrhea, ulceration in the oral cavity and others. Globally, the most reported and widely studied animal related to EHDV is the White-tailed deer as it is the most susceptible animal to EHDV while other species such as cattle may also be infected with less severe clinical signs. However, there are limited studies of EHDV in Malaysia, especially in goats. Therefore, the main objective of this study is to serve as a baseline study for the serological detection of EHDV in goats in Malaysia. For this study, competitive ELISA technique is used to detect the antibody against EHDV VP7 protein. This method is WOAH’s current preferred diagnostic method as it does not cause cross -reactivity between EHDV and Bluetongue virus. In this study, blood samples from 2 farms (total: 40 goats) were collected for the detection of the antibodies in which 3 out of 40 goats were EHDV positive. EHDV is one of the economically important diseases in ruminants as it could lead to economic losses and reduced productivity among infected animals. Therefore, this study can be set as a preliminary study of EHDV status in goats especially in Malaysia for future study. In addition to that, early detection allows faster intervention to control this disease. Keywords: EHDV, WOAH, c-ELISA, VP7 protein
format Undergraduate Final Project Report
author Maria Fazira Mohd Zulkefli
spellingShingle Maria Fazira Mohd Zulkefli
Serological Detection Of Epizootic Hemorrhagic Disease Virus (Ehdv) And Associated Risk Factors In Goats In Kelantan, Malaysia.
author_facet Maria Fazira Mohd Zulkefli
author_sort Maria Fazira Mohd Zulkefli
title Serological Detection Of Epizootic Hemorrhagic Disease Virus (Ehdv) And Associated Risk Factors In Goats In Kelantan, Malaysia.
title_short Serological Detection Of Epizootic Hemorrhagic Disease Virus (Ehdv) And Associated Risk Factors In Goats In Kelantan, Malaysia.
title_full Serological Detection Of Epizootic Hemorrhagic Disease Virus (Ehdv) And Associated Risk Factors In Goats In Kelantan, Malaysia.
title_fullStr Serological Detection Of Epizootic Hemorrhagic Disease Virus (Ehdv) And Associated Risk Factors In Goats In Kelantan, Malaysia.
title_full_unstemmed Serological Detection Of Epizootic Hemorrhagic Disease Virus (Ehdv) And Associated Risk Factors In Goats In Kelantan, Malaysia.
title_sort serological detection of epizootic hemorrhagic disease virus (ehdv) and associated risk factors in goats in kelantan, malaysia.
publishDate 2025
url http://discol.umk.edu.my/id/eprint/14861/1/MARIA%20FAZIRA%20MOHD%20ZULKEFLI%20D20B0091.pdf
http://discol.umk.edu.my/id/eprint/14861/
_version_ 1825819296662552576
score 13.244413

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