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Antibacterial Effect Of Ocimum Tenuiflorum And Plectranthus Amboinicus Extracts Against Methicillin-Resistant Staphylococcus Aureus (Mrsa) And Staphylococcus Aureus (Sa) Isolates

An abstract of the research paper presented to the Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, in partial requirement of the course DVT 55204 – Research Project. The growing frequency of antibiotic-resistant bacteria, including methicillin-resistant staphylococcus aureus (MRSA), wa...

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Main Author: Santhini Venggadasalam
Format: Undergraduate Final Project Report
Language:English
Published: 2024
Online Access:http://discol.umk.edu.my/id/eprint/14831/1/SANTHINI%20VENGGADASALAM_D20A0078.pdf
http://discol.umk.edu.my/id/eprint/14831/
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spelling my.umk.eprints.148312025-03-02T06:50:10Z http://discol.umk.edu.my/id/eprint/14831/ Antibacterial Effect Of Ocimum Tenuiflorum And Plectranthus Amboinicus Extracts Against Methicillin-Resistant Staphylococcus Aureus (Mrsa) And Staphylococcus Aureus (Sa) Isolates Santhini Venggadasalam An abstract of the research paper presented to the Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, in partial requirement of the course DVT 55204 – Research Project. The growing frequency of antibiotic-resistant bacteria, including methicillin-resistant staphylococcus aureus (MRSA), warrants the investigation of alternative antimicrobial agents. This study evaluates the antibacterial inhibitory effects of methanolic extracts of Ocimum tenuiflorum and Plectranthus amboinicus against staphylococcus aureus (SA) and MRSA isolates using the disc diffusion method. Statistical analysis using one-way ANOVA revealed significant differences in the antibacterial inhibitory effects among treatment groups for both SA (F(7,16) = 433.96, p < 0.001) and MRSA (F(7,16) = 304.70, p < 0.001). Among individual extracts, P. amboinicus exhibited the highest antibacterial inhibitory effect, with inhibition zones averaging 1.1 cm for SA and 1.0 cm for MRSA, attributed to bioactive compounds such as thymol and carvacrol. Combined extracts displayed synergistic antibacterial inhibitory effects, particularly with higher concentrations of P. amboinicus, but their efficacy was lower compared to selected commercial antibiotics, including enrofloxacin, cefoxitin, and gentamicin. These findings highlight the potential of plant-based antimicrobials, particularly P. amboinicus, as alternative therapies for multidrug-resistant pathogens. Future studies should focus on optimizing extraction protocols and elucidating the molecular mechanisms underlying their antibacterial properties. Keywords: Ocimum tenuiflorum, Plectranthus amboinicus, staphylococcus aureus, methicillin-resistant staphylococcus aureus, antibacterial effect, plant-based antimicrobials 2024-11-17 Undergraduate Final Project Report NonPeerReviewed text en http://discol.umk.edu.my/id/eprint/14831/1/SANTHINI%20VENGGADASALAM_D20A0078.pdf Santhini Venggadasalam (2024) Antibacterial Effect Of Ocimum Tenuiflorum And Plectranthus Amboinicus Extracts Against Methicillin-Resistant Staphylococcus Aureus (Mrsa) And Staphylococcus Aureus (Sa) Isolates. Final Year Project thesis, Universiti Malaysia Kelantan. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
language English
description An abstract of the research paper presented to the Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, in partial requirement of the course DVT 55204 – Research Project. The growing frequency of antibiotic-resistant bacteria, including methicillin-resistant staphylococcus aureus (MRSA), warrants the investigation of alternative antimicrobial agents. This study evaluates the antibacterial inhibitory effects of methanolic extracts of Ocimum tenuiflorum and Plectranthus amboinicus against staphylococcus aureus (SA) and MRSA isolates using the disc diffusion method. Statistical analysis using one-way ANOVA revealed significant differences in the antibacterial inhibitory effects among treatment groups for both SA (F(7,16) = 433.96, p < 0.001) and MRSA (F(7,16) = 304.70, p < 0.001). Among individual extracts, P. amboinicus exhibited the highest antibacterial inhibitory effect, with inhibition zones averaging 1.1 cm for SA and 1.0 cm for MRSA, attributed to bioactive compounds such as thymol and carvacrol. Combined extracts displayed synergistic antibacterial inhibitory effects, particularly with higher concentrations of P. amboinicus, but their efficacy was lower compared to selected commercial antibiotics, including enrofloxacin, cefoxitin, and gentamicin. These findings highlight the potential of plant-based antimicrobials, particularly P. amboinicus, as alternative therapies for multidrug-resistant pathogens. Future studies should focus on optimizing extraction protocols and elucidating the molecular mechanisms underlying their antibacterial properties. Keywords: Ocimum tenuiflorum, Plectranthus amboinicus, staphylococcus aureus, methicillin-resistant staphylococcus aureus, antibacterial effect, plant-based antimicrobials
format Undergraduate Final Project Report
author Santhini Venggadasalam
spellingShingle Santhini Venggadasalam
Antibacterial Effect Of Ocimum Tenuiflorum And Plectranthus Amboinicus Extracts Against Methicillin-Resistant Staphylococcus Aureus (Mrsa) And Staphylococcus Aureus (Sa) Isolates
author_facet Santhini Venggadasalam
author_sort Santhini Venggadasalam
title Antibacterial Effect Of Ocimum Tenuiflorum And Plectranthus Amboinicus Extracts Against Methicillin-Resistant Staphylococcus Aureus (Mrsa) And Staphylococcus Aureus (Sa) Isolates
title_short Antibacterial Effect Of Ocimum Tenuiflorum And Plectranthus Amboinicus Extracts Against Methicillin-Resistant Staphylococcus Aureus (Mrsa) And Staphylococcus Aureus (Sa) Isolates
title_full Antibacterial Effect Of Ocimum Tenuiflorum And Plectranthus Amboinicus Extracts Against Methicillin-Resistant Staphylococcus Aureus (Mrsa) And Staphylococcus Aureus (Sa) Isolates
title_fullStr Antibacterial Effect Of Ocimum Tenuiflorum And Plectranthus Amboinicus Extracts Against Methicillin-Resistant Staphylococcus Aureus (Mrsa) And Staphylococcus Aureus (Sa) Isolates
title_full_unstemmed Antibacterial Effect Of Ocimum Tenuiflorum And Plectranthus Amboinicus Extracts Against Methicillin-Resistant Staphylococcus Aureus (Mrsa) And Staphylococcus Aureus (Sa) Isolates
title_sort antibacterial effect of ocimum tenuiflorum and plectranthus amboinicus extracts against methicillin-resistant staphylococcus aureus (mrsa) and staphylococcus aureus (sa) isolates
publishDate 2024
url http://discol.umk.edu.my/id/eprint/14831/1/SANTHINI%20VENGGADASALAM_D20A0078.pdf
http://discol.umk.edu.my/id/eprint/14831/
_version_ 1825819292077129728
score 13.244413

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