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Application of anaerobic digestion model no 1 in biohydrogen optimization using palm oil mill effluent: effect of pretreatment method

Biohydrogen is an environmentally friendly and sustainable alternative energy carrier to fossil fuels. However, low yield and inconsistent processes have hindered its large-scale production. This research aims to address these challenges by increasing the reduced sugar (RS) content of Palm Oil Mill...

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Main Author: Ahmad Zul Izzi Fauzi
Format: UMK Etheses
Language:English
Published: 2023
Online Access:http://discol.umk.edu.my/id/eprint/14280/1/Ahmad%20Zul%20Izzi%20Bin%20Fauzi.pdf
http://discol.umk.edu.my/id/eprint/14280/
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spelling my.umk.eprints.142802024-07-29T03:22:21Z http://discol.umk.edu.my/id/eprint/14280/ Application of anaerobic digestion model no 1 in biohydrogen optimization using palm oil mill effluent: effect of pretreatment method Ahmad Zul Izzi Fauzi Biohydrogen is an environmentally friendly and sustainable alternative energy carrier to fossil fuels. However, low yield and inconsistent processes have hindered its large-scale production. This research aims to address these challenges by increasing the reduced sugar (RS) content of Palm Oil Mill Effluent (POME), enhancing biohydrogen yield in subsequent fermentation processes, developing a process model, and determining the dominant microbial species in the effluent. The work is divided into four main parts. The first part examines the effects of pretreatment on RS yield using acid, alkaline, and enzymatic methods. The second part focuses on biohydrogen production under thermophilic conditions using the pretreated POME as a substrate and digested sludge as inoculum. The optimization of both parts is achieved using Box-Behnken Design in a batch process. In the third part, kinetic modelling uses the modified Anaerobic Digestion Model No. 1 (ADM1) to describe the biochemical and physicochemical processes by fitting experimental data on hydrogen evolution, RS degradation, and volatile fatty acid (VFA) formation. Finally, the fourth part involves screening the dominant microbial community responsible for biohydrogen conversion using 16S rDNA sequencing methods. Results indicate that the pretreatment method improved RS recovery from acid, alkaline, and enzymatic pretreatment by 13%, 9.35%, and 182%, respectively. Optimization of the biohydrogen production process at a temperature of 55°C, mixing speed of 150 rpm, chemical oxygen demand (COD) concentration of 39,706 mg/L, seed content of 13.64%, and an initial pH of 6 significantly increased the biohydrogen production potential (Hmax) by five-fold (444 mL). The ADM1-based model shows a good fit for all models (hydrogen evolution, RS degradation, and VFA formation) with a high R2 above 0.94 and a small standard error of ≤ 0.2. The models were highly significant, with P-values far less than 0.05. Furthermore, the taxonomic analysis revealed that Thermoanaerobacterium thermosaccharolyticum species was the predominant hydrogenproducing bacterium among the diverse microbial genera. These findings provide a good basis for the further development of biohydrogen production from POME. The proposed process design, optimization, and control could lead to direct implementation in a largescale plant. 2023 UMK Etheses NonPeerReviewed text en http://discol.umk.edu.my/id/eprint/14280/1/Ahmad%20Zul%20Izzi%20Bin%20Fauzi.pdf Ahmad Zul Izzi Fauzi (2023) Application of anaerobic digestion model no 1 in biohydrogen optimization using palm oil mill effluent: effect of pretreatment method. Doctoral thesis, Universiti Malaysia Kelantan. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
language English
description Biohydrogen is an environmentally friendly and sustainable alternative energy carrier to fossil fuels. However, low yield and inconsistent processes have hindered its large-scale production. This research aims to address these challenges by increasing the reduced sugar (RS) content of Palm Oil Mill Effluent (POME), enhancing biohydrogen yield in subsequent fermentation processes, developing a process model, and determining the dominant microbial species in the effluent. The work is divided into four main parts. The first part examines the effects of pretreatment on RS yield using acid, alkaline, and enzymatic methods. The second part focuses on biohydrogen production under thermophilic conditions using the pretreated POME as a substrate and digested sludge as inoculum. The optimization of both parts is achieved using Box-Behnken Design in a batch process. In the third part, kinetic modelling uses the modified Anaerobic Digestion Model No. 1 (ADM1) to describe the biochemical and physicochemical processes by fitting experimental data on hydrogen evolution, RS degradation, and volatile fatty acid (VFA) formation. Finally, the fourth part involves screening the dominant microbial community responsible for biohydrogen conversion using 16S rDNA sequencing methods. Results indicate that the pretreatment method improved RS recovery from acid, alkaline, and enzymatic pretreatment by 13%, 9.35%, and 182%, respectively. Optimization of the biohydrogen production process at a temperature of 55°C, mixing speed of 150 rpm, chemical oxygen demand (COD) concentration of 39,706 mg/L, seed content of 13.64%, and an initial pH of 6 significantly increased the biohydrogen production potential (Hmax) by five-fold (444 mL). The ADM1-based model shows a good fit for all models (hydrogen evolution, RS degradation, and VFA formation) with a high R2 above 0.94 and a small standard error of ≤ 0.2. The models were highly significant, with P-values far less than 0.05. Furthermore, the taxonomic analysis revealed that Thermoanaerobacterium thermosaccharolyticum species was the predominant hydrogenproducing bacterium among the diverse microbial genera. These findings provide a good basis for the further development of biohydrogen production from POME. The proposed process design, optimization, and control could lead to direct implementation in a largescale plant.
format UMK Etheses
author Ahmad Zul Izzi Fauzi
spellingShingle Ahmad Zul Izzi Fauzi
Application of anaerobic digestion model no 1 in biohydrogen optimization using palm oil mill effluent: effect of pretreatment method
author_facet Ahmad Zul Izzi Fauzi
author_sort Ahmad Zul Izzi Fauzi
title Application of anaerobic digestion model no 1 in biohydrogen optimization using palm oil mill effluent: effect of pretreatment method
title_short Application of anaerobic digestion model no 1 in biohydrogen optimization using palm oil mill effluent: effect of pretreatment method
title_full Application of anaerobic digestion model no 1 in biohydrogen optimization using palm oil mill effluent: effect of pretreatment method
title_fullStr Application of anaerobic digestion model no 1 in biohydrogen optimization using palm oil mill effluent: effect of pretreatment method
title_full_unstemmed Application of anaerobic digestion model no 1 in biohydrogen optimization using palm oil mill effluent: effect of pretreatment method
title_sort application of anaerobic digestion model no 1 in biohydrogen optimization using palm oil mill effluent: effect of pretreatment method
publishDate 2023
url http://discol.umk.edu.my/id/eprint/14280/1/Ahmad%20Zul%20Izzi%20Bin%20Fauzi.pdf
http://discol.umk.edu.my/id/eprint/14280/
_version_ 1806436787947569152
score 13.211869

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