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Molecular detection of leptospira spp. In bovine kidney In Kota Bharu, Kelantan

Leptospirosis is a bacterial disease that has zoonotic potential, affecting both humans and animals. The aim of this study was to detect the Leptospira spp. and determine the predominant species related in the bovine kidney samples collected from selected wet markets in Kota Bharu, Kelantan. Further...

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Main Author: Ong Siew Zee
Format: Undergraduate Final Project Report
Language:English
Published: 2022
Online Access:http://discol.umk.edu.my/id/eprint/10667/1/ONG%20SIEW%20ZEE.pdf
http://discol.umk.edu.my/id/eprint/10667/
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spelling my.umk.eprints.106672023-04-02T04:36:38Z http://discol.umk.edu.my/id/eprint/10667/ Molecular detection of leptospira spp. In bovine kidney In Kota Bharu, Kelantan Ong Siew Zee Leptospirosis is a bacterial disease that has zoonotic potential, affecting both humans and animals. The aim of this study was to detect the Leptospira spp. and determine the predominant species related in the bovine kidney samples collected from selected wet markets in Kota Bharu, Kelantan. Furthermore, to investigate the pathological alterations of bovine kidneys related to the disease. A total of 50 bovine kidney samples were collected from four wet markets across Kota Bharu area and the samples were tested using polymerase chain reaction (PCR) assay. From the results, five samples (10%) were found to be positive for 16S rRNA gene, while one (2%) sample showed positive results for LipL32 gene. The further sequencing of the positive samples for both 16S rRNA and LipL32 gene were revealed as Leptospira borgpetersenii, which is known to be a pathogenic species in cattle. Furthermore, the bovine kidneys that gave positive PCR results showed relevant histopathological lesions of bovine leptospirosis, consisting of interstitial nephritis, glomerular atrophy and tubular necrosis. In conclusion, this study demonstrated the presence of pathogenic Leptospira spp. in bovine kidneys, which caused a series of histopathological changes and could pose zoonotic potential to the public. Keywords: Cattle, Histopathology, Leptospirosis, LipL32, PCR, 16S rRNA 2022-06 Undergraduate Final Project Report NonPeerReviewed text en http://discol.umk.edu.my/id/eprint/10667/1/ONG%20SIEW%20ZEE.pdf Ong Siew Zee (2022) Molecular detection of leptospira spp. In bovine kidney In Kota Bharu, Kelantan. Final Year Project thesis, Universiti Malaysia Kelantan. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
language English
description Leptospirosis is a bacterial disease that has zoonotic potential, affecting both humans and animals. The aim of this study was to detect the Leptospira spp. and determine the predominant species related in the bovine kidney samples collected from selected wet markets in Kota Bharu, Kelantan. Furthermore, to investigate the pathological alterations of bovine kidneys related to the disease. A total of 50 bovine kidney samples were collected from four wet markets across Kota Bharu area and the samples were tested using polymerase chain reaction (PCR) assay. From the results, five samples (10%) were found to be positive for 16S rRNA gene, while one (2%) sample showed positive results for LipL32 gene. The further sequencing of the positive samples for both 16S rRNA and LipL32 gene were revealed as Leptospira borgpetersenii, which is known to be a pathogenic species in cattle. Furthermore, the bovine kidneys that gave positive PCR results showed relevant histopathological lesions of bovine leptospirosis, consisting of interstitial nephritis, glomerular atrophy and tubular necrosis. In conclusion, this study demonstrated the presence of pathogenic Leptospira spp. in bovine kidneys, which caused a series of histopathological changes and could pose zoonotic potential to the public. Keywords: Cattle, Histopathology, Leptospirosis, LipL32, PCR, 16S rRNA
format Undergraduate Final Project Report
author Ong Siew Zee
spellingShingle Ong Siew Zee
Molecular detection of leptospira spp. In bovine kidney In Kota Bharu, Kelantan
author_facet Ong Siew Zee
author_sort Ong Siew Zee
title Molecular detection of leptospira spp. In bovine kidney In Kota Bharu, Kelantan
title_short Molecular detection of leptospira spp. In bovine kidney In Kota Bharu, Kelantan
title_full Molecular detection of leptospira spp. In bovine kidney In Kota Bharu, Kelantan
title_fullStr Molecular detection of leptospira spp. In bovine kidney In Kota Bharu, Kelantan
title_full_unstemmed Molecular detection of leptospira spp. In bovine kidney In Kota Bharu, Kelantan
title_sort molecular detection of leptospira spp. in bovine kidney in kota bharu, kelantan
publishDate 2022
url http://discol.umk.edu.my/id/eprint/10667/1/ONG%20SIEW%20ZEE.pdf
http://discol.umk.edu.my/id/eprint/10667/
_version_ 1763304273863507968
score 13.211869

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