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Molecular detection of bartonella spp. In bat flies in east-coast malaysia

Bats are recognized as a major reservoir for numerous pathogens including Bartonella spp. and it is one of the emerging zoonotic bacterial diseases that can be transmitted to humans and may cause various unspecific clinical manifestations. Thus, Bartonellosis is rarely diagnosed and regarded as negl...

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Main Author: Azra Hafizah Kamar
Format: Undergraduate Final Project Report
Language:English
Published: 2022
Online Access:http://discol.umk.edu.my/id/eprint/10625/1/AZRA%20HAFIZAH%20BINTI%20KAMAR.pdf
http://discol.umk.edu.my/id/eprint/10625/
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spelling my.umk.eprints.106252023-04-02T04:55:47Z http://discol.umk.edu.my/id/eprint/10625/ Molecular detection of bartonella spp. In bat flies in east-coast malaysia Azra Hafizah Kamar Bats are recognized as a major reservoir for numerous pathogens including Bartonella spp. and it is one of the emerging zoonotic bacterial diseases that can be transmitted to humans and may cause various unspecific clinical manifestations. Thus, Bartonellosis is rarely diagnosed and regarded as neglected vector borne disease (VBD). Bat flies have been hypothesized to be a vector in transmission of pathogens among bats. They are host-specific, which reduces the likelihood of pathogen transmission across bat species, however it is still likely to maintain the pathogen loads within its host species populations. To explore more on this neglected bacteria and its presence in bat flies, bat flies samples collected from East Coast Malaysia were used for molecular detection and sequences analysis of Bartonella spp.. A percentage of 38.71% bats were infested with bat flies from Terengganu and Kelantan and no bat fly was found in Pahang collection site. Both bat flies families, Nycteribiidae (79.59%) and Streblidae (20%) were identified. The bat flies were pooled into 37 samples and 70.27% were positive for Bartonella spp. by PCR and four positive samples were randomly selected for sequence analysis, all samples had 95% identity to Bartonella spp. from two different locations, which were LR9Brazil and B40400African strains. They were regarded as new species as the citrate synthase (gltA) sequence share less than 96% similarity to previously identified species and the obtained findings also suggest that the bat flies in East Coast Malaysia serve as a reservoir for a zoonotic potential Bartonella spp. Keywords: bats, Bartonella spp., bat flies, PCR, sequence analysis 2022-09 Undergraduate Final Project Report NonPeerReviewed text en http://discol.umk.edu.my/id/eprint/10625/1/AZRA%20HAFIZAH%20BINTI%20KAMAR.pdf Azra Hafizah Kamar (2022) Molecular detection of bartonella spp. In bat flies in east-coast malaysia. Final Year Project thesis, Universiti Malaysia Kelantan. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
language English
description Bats are recognized as a major reservoir for numerous pathogens including Bartonella spp. and it is one of the emerging zoonotic bacterial diseases that can be transmitted to humans and may cause various unspecific clinical manifestations. Thus, Bartonellosis is rarely diagnosed and regarded as neglected vector borne disease (VBD). Bat flies have been hypothesized to be a vector in transmission of pathogens among bats. They are host-specific, which reduces the likelihood of pathogen transmission across bat species, however it is still likely to maintain the pathogen loads within its host species populations. To explore more on this neglected bacteria and its presence in bat flies, bat flies samples collected from East Coast Malaysia were used for molecular detection and sequences analysis of Bartonella spp.. A percentage of 38.71% bats were infested with bat flies from Terengganu and Kelantan and no bat fly was found in Pahang collection site. Both bat flies families, Nycteribiidae (79.59%) and Streblidae (20%) were identified. The bat flies were pooled into 37 samples and 70.27% were positive for Bartonella spp. by PCR and four positive samples were randomly selected for sequence analysis, all samples had 95% identity to Bartonella spp. from two different locations, which were LR9Brazil and B40400African strains. They were regarded as new species as the citrate synthase (gltA) sequence share less than 96% similarity to previously identified species and the obtained findings also suggest that the bat flies in East Coast Malaysia serve as a reservoir for a zoonotic potential Bartonella spp. Keywords: bats, Bartonella spp., bat flies, PCR, sequence analysis
format Undergraduate Final Project Report
author Azra Hafizah Kamar
spellingShingle Azra Hafizah Kamar
Molecular detection of bartonella spp. In bat flies in east-coast malaysia
author_facet Azra Hafizah Kamar
author_sort Azra Hafizah Kamar
title Molecular detection of bartonella spp. In bat flies in east-coast malaysia
title_short Molecular detection of bartonella spp. In bat flies in east-coast malaysia
title_full Molecular detection of bartonella spp. In bat flies in east-coast malaysia
title_fullStr Molecular detection of bartonella spp. In bat flies in east-coast malaysia
title_full_unstemmed Molecular detection of bartonella spp. In bat flies in east-coast malaysia
title_sort molecular detection of bartonella spp. in bat flies in east-coast malaysia
publishDate 2022
url http://discol.umk.edu.my/id/eprint/10625/1/AZRA%20HAFIZAH%20BINTI%20KAMAR.pdf
http://discol.umk.edu.my/id/eprint/10625/
_version_ 1763304268036571136
score 13.211869

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