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Characterization of bacteria coliform in smoked and live Asian Clam (corbicula fluminea) sold in Kota Bharu, Kelantan.

Up to date, there is no database information on the characterization of bacteria coliform isolated from smoked and live Asian Clam (Corbicula fluminea) in Malaysia. Thus, this study was carried out to provide information on phenotyphic characteristics, antibiogram and heavy metal resistance pattern,...

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Main Author: Zalina Che Manan
Format: UMK Etheses
Language:English
Published: 2014
Subjects:
QD Chemistry
Online Access:http://discol.umk.edu.my/id/eprint/10334/1/Zalina%20binti%20Che%20Manan.pdf
http://discol.umk.edu.my/id/eprint/10334/
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spelling my.umk.eprints.103342022-08-15T05:20:59Z http://discol.umk.edu.my/id/eprint/10334/ Characterization of bacteria coliform in smoked and live Asian Clam (corbicula fluminea) sold in Kota Bharu, Kelantan. Zalina Che Manan QD Chemistry Up to date, there is no database information on the characterization of bacteria coliform isolated from smoked and live Asian Clam (Corbicula fluminea) in Malaysia. Thus, this study was carried out to provide information on phenotyphic characteristics, antibiogram and heavy metal resistance pattern, as well as the genotypes of the bacteria coliform. Current database information may useful for future studies in clinical analysis especially in epidemiological study, prophylactic purposes, and public health management plans. Bacteria coliform were isolated from smoked and live Asian Clam using Eosin Methylene Blue (EMB) agar, Thiosulphate Citrate Bile Salt (TCBS) agar, Glutamate Starch Pseudomonas (GSP) agar, Xylose Lysine Deoxycholate (XLD) agar and Cytophaga agar (CA). Bacterial identification was conducted using conventional biochemical tests and confirmed with a commercial identification kit. The antimicrobial resistance pattern was tested using a disk diffusion method, the heavy metal resistance pattern (cadmium, copper, mercury, and zinc) was determined using two-fold agar dilution method. The genomic properties of the bacteria were studied by a random amplified polymorphic DNA – polymerase chain reaction (RAPD-PCR). By using four primers (785, 786, 795, and OPA- 11), matrix data were analyzed using NTSYS software (version 2.1). Dendrogram constructed from unweighted pair-group method with arithmetic means (UPGMA). 2014 UMK Etheses NonPeerReviewed text en http://discol.umk.edu.my/id/eprint/10334/1/Zalina%20binti%20Che%20Manan.pdf Zalina Che Manan (2014) Characterization of bacteria coliform in smoked and live Asian Clam (corbicula fluminea) sold in Kota Bharu, Kelantan. Masters thesis, Universiti Malaysia Kelantan.
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Zalina Che Manan
Characterization of bacteria coliform in smoked and live Asian Clam (corbicula fluminea) sold in Kota Bharu, Kelantan.
description Up to date, there is no database information on the characterization of bacteria coliform isolated from smoked and live Asian Clam (Corbicula fluminea) in Malaysia. Thus, this study was carried out to provide information on phenotyphic characteristics, antibiogram and heavy metal resistance pattern, as well as the genotypes of the bacteria coliform. Current database information may useful for future studies in clinical analysis especially in epidemiological study, prophylactic purposes, and public health management plans. Bacteria coliform were isolated from smoked and live Asian Clam using Eosin Methylene Blue (EMB) agar, Thiosulphate Citrate Bile Salt (TCBS) agar, Glutamate Starch Pseudomonas (GSP) agar, Xylose Lysine Deoxycholate (XLD) agar and Cytophaga agar (CA). Bacterial identification was conducted using conventional biochemical tests and confirmed with a commercial identification kit. The antimicrobial resistance pattern was tested using a disk diffusion method, the heavy metal resistance pattern (cadmium, copper, mercury, and zinc) was determined using two-fold agar dilution method. The genomic properties of the bacteria were studied by a random amplified polymorphic DNA – polymerase chain reaction (RAPD-PCR). By using four primers (785, 786, 795, and OPA- 11), matrix data were analyzed using NTSYS software (version 2.1). Dendrogram constructed from unweighted pair-group method with arithmetic means (UPGMA).
format UMK Etheses
author Zalina Che Manan
author_facet Zalina Che Manan
author_sort Zalina Che Manan
title Characterization of bacteria coliform in smoked and live Asian Clam (corbicula fluminea) sold in Kota Bharu, Kelantan.
title_short Characterization of bacteria coliform in smoked and live Asian Clam (corbicula fluminea) sold in Kota Bharu, Kelantan.
title_full Characterization of bacteria coliform in smoked and live Asian Clam (corbicula fluminea) sold in Kota Bharu, Kelantan.
title_fullStr Characterization of bacteria coliform in smoked and live Asian Clam (corbicula fluminea) sold in Kota Bharu, Kelantan.
title_full_unstemmed Characterization of bacteria coliform in smoked and live Asian Clam (corbicula fluminea) sold in Kota Bharu, Kelantan.
title_sort characterization of bacteria coliform in smoked and live asian clam (corbicula fluminea) sold in kota bharu, kelantan.
publishDate 2014
url http://discol.umk.edu.my/id/eprint/10334/1/Zalina%20binti%20Che%20Manan.pdf
http://discol.umk.edu.my/id/eprint/10334/
_version_ 1763304232549613568
score 13.211869

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