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In vitro direct and indirect organogenesis and plant regeneration of Kenaf (Hibiscus Cannabinus L) var. KB6.

As an important fiber crop, many potential applications of kenaf are being identified and developed in 21 century, especially in developed countries such as America, Japan, and France and Malaysia as well. The present study report a protocol for the efficient in vitro propagation of kenaf (H.cannabi...

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Main Author: Zeti Ermiena Surya Mat Hussin
Format: UMK Etheses
Language:English
Published: 2016
Subjects:
QK Botany
Online Access:http://discol.umk.edu.my/id/eprint/10293/1/ZETI%20ERMIENA%20SURYA%20BT%20MAT%20HUSSIN.pdf
http://discol.umk.edu.my/id/eprint/10293/
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spelling my.umk.eprints.102932022-08-16T04:23:38Z http://discol.umk.edu.my/id/eprint/10293/ In vitro direct and indirect organogenesis and plant regeneration of Kenaf (Hibiscus Cannabinus L) var. KB6. Zeti Ermiena Surya Mat Hussin QK Botany As an important fiber crop, many potential applications of kenaf are being identified and developed in 21 century, especially in developed countries such as America, Japan, and France and Malaysia as well. The present study report a protocol for the efficient in vitro propagation of kenaf (H.cannabinus L) to initiate multiple shoot from mother plant part (shoot, petiole, node and leaf) through direct and indirect organogenesis using MS medium + BAP + IAA for direct organogenesis and MS medium + KN+2,4-D for indirect organogenesis to get callus and after 8 weeks, the calli were put in the MS medium + BAP +IAA for shoot induction. The highest number of shoots produced from node explants part via direct organogenesis (16.33/explants) in MS medium + 0.5 mg/l BAP +0.05 mg/l IAA. The highest percentage explants forming callus and callus generate into shoot also from node explants part which was induced 75% callus from explants and 73.33% of callus turn into shoot in MS medium + 0.1 mg/l BAP. Several subcultures were drived in order to enhance the multiplication rate. The treatments have their significant different with others. The shoots then were transferred to the root induction medium. Shoots showed the vigorous roots in the MS basal medium. The in vitro rooted plantlets were acclimatized in sand+ coco pit + vermiculite with ratio 3:2:2 and were covered with hole container for 0-15 days to test the effect of humidity to plantlets. The 9th-15th days explants covered with container showed 100% of survive. Survive well rooted plantlets were transferred to the field. Plants grew well into maturity without any remarkable morphological variations within the treatments. 2016 UMK Etheses NonPeerReviewed text en http://discol.umk.edu.my/id/eprint/10293/1/ZETI%20ERMIENA%20SURYA%20BT%20MAT%20HUSSIN.pdf Zeti Ermiena Surya Mat Hussin (2016) In vitro direct and indirect organogenesis and plant regeneration of Kenaf (Hibiscus Cannabinus L) var. KB6. Masters thesis, Universiti Malaysia Kelantan.
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
language English
topic QK Botany
spellingShingle QK Botany
Zeti Ermiena Surya Mat Hussin
In vitro direct and indirect organogenesis and plant regeneration of Kenaf (Hibiscus Cannabinus L) var. KB6.
description As an important fiber crop, many potential applications of kenaf are being identified and developed in 21 century, especially in developed countries such as America, Japan, and France and Malaysia as well. The present study report a protocol for the efficient in vitro propagation of kenaf (H.cannabinus L) to initiate multiple shoot from mother plant part (shoot, petiole, node and leaf) through direct and indirect organogenesis using MS medium + BAP + IAA for direct organogenesis and MS medium + KN+2,4-D for indirect organogenesis to get callus and after 8 weeks, the calli were put in the MS medium + BAP +IAA for shoot induction. The highest number of shoots produced from node explants part via direct organogenesis (16.33/explants) in MS medium + 0.5 mg/l BAP +0.05 mg/l IAA. The highest percentage explants forming callus and callus generate into shoot also from node explants part which was induced 75% callus from explants and 73.33% of callus turn into shoot in MS medium + 0.1 mg/l BAP. Several subcultures were drived in order to enhance the multiplication rate. The treatments have their significant different with others. The shoots then were transferred to the root induction medium. Shoots showed the vigorous roots in the MS basal medium. The in vitro rooted plantlets were acclimatized in sand+ coco pit + vermiculite with ratio 3:2:2 and were covered with hole container for 0-15 days to test the effect of humidity to plantlets. The 9th-15th days explants covered with container showed 100% of survive. Survive well rooted plantlets were transferred to the field. Plants grew well into maturity without any remarkable morphological variations within the treatments.
format UMK Etheses
author Zeti Ermiena Surya Mat Hussin
author_facet Zeti Ermiena Surya Mat Hussin
author_sort Zeti Ermiena Surya Mat Hussin
title In vitro direct and indirect organogenesis and plant regeneration of Kenaf (Hibiscus Cannabinus L) var. KB6.
title_short In vitro direct and indirect organogenesis and plant regeneration of Kenaf (Hibiscus Cannabinus L) var. KB6.
title_full In vitro direct and indirect organogenesis and plant regeneration of Kenaf (Hibiscus Cannabinus L) var. KB6.
title_fullStr In vitro direct and indirect organogenesis and plant regeneration of Kenaf (Hibiscus Cannabinus L) var. KB6.
title_full_unstemmed In vitro direct and indirect organogenesis and plant regeneration of Kenaf (Hibiscus Cannabinus L) var. KB6.
title_sort in vitro direct and indirect organogenesis and plant regeneration of kenaf (hibiscus cannabinus l) var. kb6.
publishDate 2016
url http://discol.umk.edu.my/id/eprint/10293/1/ZETI%20ERMIENA%20SURYA%20BT%20MAT%20HUSSIN.pdf
http://discol.umk.edu.my/id/eprint/10293/
_version_ 1763304228544053248
score 13.211869

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