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Utilisation of DNA barcoding in assessing the diversity of bats based on taxonomic records and identifying their plant-based diet in Peninsular Malaysia / Lim Voon Ching

In Peninsular Malaysia, the diversity of bats was previously assessed through morphological identification of captured bats while the diet of plant-visiting bats were examined through morphological identification of seeds and pollen grains collected from bats. Yet morphological identification is...

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Main Author: Lim , Voon Ching
Format: Thesis
Published: 2018
Subjects:
Q Science (General)
QH301 Biology
Online Access:http://studentsrepo.um.edu.my/8991/1/Lim_Voon_Ching.pdf
http://studentsrepo.um.edu.my/8991/8/voon_ching.pdf
http://studentsrepo.um.edu.my/8991/
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spelling my.um.stud.89912021-03-01T22:52:14Z Utilisation of DNA barcoding in assessing the diversity of bats based on taxonomic records and identifying their plant-based diet in Peninsular Malaysia / Lim Voon Ching Lim , Voon Ching Q Science (General) QH301 Biology In Peninsular Malaysia, the diversity of bats was previously assessed through morphological identification of captured bats while the diet of plant-visiting bats were examined through morphological identification of seeds and pollen grains collected from bats. Yet morphological identification is often of limited service when applied to identification of morphologically similar bat and plant species. The objective of this research is to use a molecular approach, DNA barcoding to review the diversity of bats and their plant-based diet in Peninsular Malaysia. Through literature review and Neighbour-Joining analyses of DNA barcodes available from bats sampled in Peninsular Malaysia, at least 110 bat species have been documented in the region and eighteen of them are species complex which deserve further investigation. The diet of frugivorous bat, Cynopterus brachyotis, at secondary forest, oil palm plantation and urban area were compared by identifying pulps and seeds found in the bats’ faeces using DNA barcoding. Native and introduced plants were detected from bat faeces at all sampling sites, suggesting the dual role of C. brachyotis in dispersing (i) native plants which aid in forest regeneration, and (ii) introduced plants which potentially facilitate their invasion. The diet of nectarivorous bat, Eonycteris spelaea at urban area was examined by identifying the plant material present in the bat faeces using DNA metabarcoding. Many plant species which were detected from the bat faeces have not been reported in previous dietary studies of E. spelaea including ferns and figs, consequently suggesting that E. spelaea may not be specialised nectarivore. Therefore, the use of DNA barcoding has highlighted the taxonomic uncertainties in bats and provided new insights into diet of plant-visiting bats. 2018-07 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/8991/1/Lim_Voon_Ching.pdf application/pdf http://studentsrepo.um.edu.my/8991/8/voon_ching.pdf Lim , Voon Ching (2018) Utilisation of DNA barcoding in assessing the diversity of bats based on taxonomic records and identifying their plant-based diet in Peninsular Malaysia / Lim Voon Ching. PhD thesis, University of Malaya. http://studentsrepo.um.edu.my/8991/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic Q Science (General)
QH301 Biology
spellingShingle Q Science (General)
QH301 Biology
Lim , Voon Ching
Utilisation of DNA barcoding in assessing the diversity of bats based on taxonomic records and identifying their plant-based diet in Peninsular Malaysia / Lim Voon Ching
description In Peninsular Malaysia, the diversity of bats was previously assessed through morphological identification of captured bats while the diet of plant-visiting bats were examined through morphological identification of seeds and pollen grains collected from bats. Yet morphological identification is often of limited service when applied to identification of morphologically similar bat and plant species. The objective of this research is to use a molecular approach, DNA barcoding to review the diversity of bats and their plant-based diet in Peninsular Malaysia. Through literature review and Neighbour-Joining analyses of DNA barcodes available from bats sampled in Peninsular Malaysia, at least 110 bat species have been documented in the region and eighteen of them are species complex which deserve further investigation. The diet of frugivorous bat, Cynopterus brachyotis, at secondary forest, oil palm plantation and urban area were compared by identifying pulps and seeds found in the bats’ faeces using DNA barcoding. Native and introduced plants were detected from bat faeces at all sampling sites, suggesting the dual role of C. brachyotis in dispersing (i) native plants which aid in forest regeneration, and (ii) introduced plants which potentially facilitate their invasion. The diet of nectarivorous bat, Eonycteris spelaea at urban area was examined by identifying the plant material present in the bat faeces using DNA metabarcoding. Many plant species which were detected from the bat faeces have not been reported in previous dietary studies of E. spelaea including ferns and figs, consequently suggesting that E. spelaea may not be specialised nectarivore. Therefore, the use of DNA barcoding has highlighted the taxonomic uncertainties in bats and provided new insights into diet of plant-visiting bats.
format Thesis
author Lim , Voon Ching
author_facet Lim , Voon Ching
author_sort Lim , Voon Ching
title Utilisation of DNA barcoding in assessing the diversity of bats based on taxonomic records and identifying their plant-based diet in Peninsular Malaysia / Lim Voon Ching
title_short Utilisation of DNA barcoding in assessing the diversity of bats based on taxonomic records and identifying their plant-based diet in Peninsular Malaysia / Lim Voon Ching
title_full Utilisation of DNA barcoding in assessing the diversity of bats based on taxonomic records and identifying their plant-based diet in Peninsular Malaysia / Lim Voon Ching
title_fullStr Utilisation of DNA barcoding in assessing the diversity of bats based on taxonomic records and identifying their plant-based diet in Peninsular Malaysia / Lim Voon Ching
title_full_unstemmed Utilisation of DNA barcoding in assessing the diversity of bats based on taxonomic records and identifying their plant-based diet in Peninsular Malaysia / Lim Voon Ching
title_sort utilisation of dna barcoding in assessing the diversity of bats based on taxonomic records and identifying their plant-based diet in peninsular malaysia / lim voon ching
publishDate 2018
url http://studentsrepo.um.edu.my/8991/1/Lim_Voon_Ching.pdf
http://studentsrepo.um.edu.my/8991/8/voon_ching.pdf
http://studentsrepo.um.edu.my/8991/
_version_ 1738506211572056064
score 13.211869

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