Characterization of AHL-type quorum sensing in Cedecea neteri SSMD04 / Tan Kian Hin
Bacteria demonstrate a form of cell-to-cell signalling for the regulation of their gene expression according to the change of population density. This is called quorum sensing. Different bacterial species utilizes different signalling molecules for quorum sensing and the most well studied quorum...
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| Format: | Thesis |
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2018
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| Online Access: | http://studentsrepo.um.edu.my/8807/1/Tan_Kian_Hin.pdf http://studentsrepo.um.edu.my/8807/6/kian_hin.pdf http://studentsrepo.um.edu.my/8807/ |
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| Summary: | Bacteria demonstrate a form of cell-to-cell signalling for the regulation of their gene
expression according to the change of population density. This is called quorum sensing.
Different bacterial species utilizes different signalling molecules for quorum sensing and
the most well studied quorum sensing system is the N-acyl homoserine lactone type
quorum sensing found commonly in Gram-negative Proteobacteria. Cedecea neteri is an
uncommonly studied bacteria in the Enterobacteriaceae family. It is a known human
pathogen with unknown etiology. In a previous study to investigate the presence of
bacteria that exhibit N-acyl homoserine lactone type quorum sensing isolated from food
sources, a strain of C. neteri SSMD04 was found to exhibit quorum sensing activity,
which was the first in this genus. By using triple quadrupole liquid chromatography mass
spectrometry, it was identified that C. neteri SSMD04 produces C4-HSL as its signalling
molecule. The gene responsible for C4-HSL production and the gene for the receptor that
binds to C4-HSL, named cneI and cneR, were later found from its genome. These genes
were found to be most closely related to a new species in the Klebsiella genus, Klebsiella
michiganensis. However, K. michiganensis has never been reported to exhibit quorum
sensing activity. A quorum sensing deficient mutant of C. neteri SSMD04 was later
created by λ Red recombineering. Through global comparative transcriptomics, it was
shown that N-acyl homoserine lactone type quorum sensing is responsible for the
modulation of its metabolism. |
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