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The effect of cinnamomum cassia on two breast cancer cell lines / Sima Kianpour Rad

The bark of the cinnamon tree (Cinnamomum cassia) is a popular culinary spice. It is also used in traditional medicine to maintain health and prevent disease. The antioxidant and anticancer activity of C. cassia was investigated using various assays. C. cassia bark was sequentially extracted with se...

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Main Author: Kianpour Rad, Sima
Format: Thesis
Published: 2014
Subjects:
R Medicine (General)
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:http://studentsrepo.um.edu.my/7098/1/simat_submitted_thesis_final_%2D_1.pdf
http://studentsrepo.um.edu.my/7098/
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spelling my.um.stud.70982017-02-04T09:40:00Z The effect of cinnamomum cassia on two breast cancer cell lines / Sima Kianpour Rad Kianpour Rad, Sima R Medicine (General) RC0254 Neoplasms. Tumors. Oncology (including Cancer) The bark of the cinnamon tree (Cinnamomum cassia) is a popular culinary spice. It is also used in traditional medicine to maintain health and prevent disease. The antioxidant and anticancer activity of C. cassia was investigated using various assays. C. cassia bark was sequentially extracted with seven solvents of varying polarity. The acetone extract of C. cassia, at 30 μg/ml, protected the mouse fibroblast cell line, 3T3-L1, from DNA damage by 45 %, as estimated by the comet assay. The acetone extract had the highest total phenolic and flavonoid content. The hexane extract of C. cassia and the two main components, trans-cinnamaldehyde and coumarin, inhibited the proliferation of two breast cancer cell lines, the estrogen-sensitive MCF-7 cells (IC50, 34 ±3.52 μg/ml) and the estrogen-insensitive MDA-MB-231 cells (IC50, 32.42 ±0.37 μg/ml). The mechanism of cell death was investigated by determining the activity of the caspases. The expression of particular apoptotic genes such as Bcl2, Akt1, p53 and Bid were investigated by real time RT-PCR. The hexane extract activated initiator caspases-8 and -9 and effector caspases-3 and -7. There was up-regulation of Bid and p53 expression. Akt1 expression was down-regulated in MDA-MB-231cells but up-regulated in MCF-7 cells, indicating partial resistance to apoptosis. The activity of the antioxidant enzymes, catalase and glutathione peroxidase in both cell lines, in response to 100 μg/ml of hexane extract, decreased in a time dependent manner, whereas that of superoxide dismutase decreased in MDA-MB-231 cells but increased in MCF-7 cells, indicating that C. cassia bark is a good source of antioxidants. Together with its anticancer and anticarcinogenic properties, it is a good supplement for maintenance of health and prevention of cancer. 2014 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/7098/1/simat_submitted_thesis_final_%2D_1.pdf Kianpour Rad, Sima (2014) The effect of cinnamomum cassia on two breast cancer cell lines / Sima Kianpour Rad. Masters thesis, University Of Malaya. http://studentsrepo.um.edu.my/7098/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic R Medicine (General)
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle R Medicine (General)
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Kianpour Rad, Sima
The effect of cinnamomum cassia on two breast cancer cell lines / Sima Kianpour Rad
description The bark of the cinnamon tree (Cinnamomum cassia) is a popular culinary spice. It is also used in traditional medicine to maintain health and prevent disease. The antioxidant and anticancer activity of C. cassia was investigated using various assays. C. cassia bark was sequentially extracted with seven solvents of varying polarity. The acetone extract of C. cassia, at 30 μg/ml, protected the mouse fibroblast cell line, 3T3-L1, from DNA damage by 45 %, as estimated by the comet assay. The acetone extract had the highest total phenolic and flavonoid content. The hexane extract of C. cassia and the two main components, trans-cinnamaldehyde and coumarin, inhibited the proliferation of two breast cancer cell lines, the estrogen-sensitive MCF-7 cells (IC50, 34 ±3.52 μg/ml) and the estrogen-insensitive MDA-MB-231 cells (IC50, 32.42 ±0.37 μg/ml). The mechanism of cell death was investigated by determining the activity of the caspases. The expression of particular apoptotic genes such as Bcl2, Akt1, p53 and Bid were investigated by real time RT-PCR. The hexane extract activated initiator caspases-8 and -9 and effector caspases-3 and -7. There was up-regulation of Bid and p53 expression. Akt1 expression was down-regulated in MDA-MB-231cells but up-regulated in MCF-7 cells, indicating partial resistance to apoptosis. The activity of the antioxidant enzymes, catalase and glutathione peroxidase in both cell lines, in response to 100 μg/ml of hexane extract, decreased in a time dependent manner, whereas that of superoxide dismutase decreased in MDA-MB-231 cells but increased in MCF-7 cells, indicating that C. cassia bark is a good source of antioxidants. Together with its anticancer and anticarcinogenic properties, it is a good supplement for maintenance of health and prevention of cancer.
format Thesis
author Kianpour Rad, Sima
author_facet Kianpour Rad, Sima
author_sort Kianpour Rad, Sima
title The effect of cinnamomum cassia on two breast cancer cell lines / Sima Kianpour Rad
title_short The effect of cinnamomum cassia on two breast cancer cell lines / Sima Kianpour Rad
title_full The effect of cinnamomum cassia on two breast cancer cell lines / Sima Kianpour Rad
title_fullStr The effect of cinnamomum cassia on two breast cancer cell lines / Sima Kianpour Rad
title_full_unstemmed The effect of cinnamomum cassia on two breast cancer cell lines / Sima Kianpour Rad
title_sort effect of cinnamomum cassia on two breast cancer cell lines / sima kianpour rad
publishDate 2014
url http://studentsrepo.um.edu.my/7098/1/simat_submitted_thesis_final_%2D_1.pdf
http://studentsrepo.um.edu.my/7098/
_version_ 1738505987631874048
score 13.211869

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