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Molecular strategies for control of cymbidium mosaic virus / Adriya binti Dzulkurnain @ Hamidi

Orchid, amongst the most popular flowers in the world, play a major role in the economy of many Asian countries. However, detrimental effects by various viruses can reduce the economic value of this horticultural crop. There have been a number of viruses known to occur in orchids globally with one o...

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Main Author: Dzulkurnain @ Hamidi, Adriya
Format: Thesis
Published: 2013
Subjects:
QH301 Biology
Online Access:http://studentsrepo.um.edu.my/5792/3/Name.pdf
http://studentsrepo.um.edu.my/5792/2/Preface.pdf
http://studentsrepo.um.edu.my/5792/1/Molecular_Strategies_For_Control_of_Cymbidium_Mosaic_Virus_Thesis.pdf
http://studentsrepo.um.edu.my/5792/
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spelling my.um.stud.57922015-07-29T08:00:49Z Molecular strategies for control of cymbidium mosaic virus / Adriya binti Dzulkurnain @ Hamidi Dzulkurnain @ Hamidi, Adriya QH301 Biology Orchid, amongst the most popular flowers in the world, play a major role in the economy of many Asian countries. However, detrimental effects by various viruses can reduce the economic value of this horticultural crop. There have been a number of viruses known to occur in orchids globally with one of the most common being Cymbidium mosaic virus (CymMV). Plant genetic engineering approaches have been successful in producing crops with viral resistance, however there has been a concern expressed that resistance resulting from single gene constructs might be rather narrow (specific to only certain virus sub-type) could easily break down. Thus this research aimed to develop a multiple strategy approach towards the control of CymMV in orchids by combining the concepts of pathogen derived resistance and antibody mediated resistance. A plant transformation vector, pCAMBIA 1304-CymMV-fwd was constructed to contain coat protein sequences from the CymMV. The construct was successfully transformed into explant samples and RT-PCR analysis showed that the pathogen derived resistance sequence was successfully expressed in plants. In a second part to the study anti-CymMV was isolated from a Toxoplasma gondii-immunized single-chain variable fragment (ScFv) antibody phage-display library. Four antibodies were isolated and analyzed through BLASTX and nucleotide BLAST but only one provided significant similarity to PDPK1 3-phosphoinositide dependent protein kinase-1 [Homo sapiens]. The two genes have the potential to be pyramided together in a single plant transformation vector, or to be introduced into plants by successive transformation, in order to develop orchids with longer term viral resistance. 2013 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/5792/3/Name.pdf application/pdf http://studentsrepo.um.edu.my/5792/2/Preface.pdf application/pdf http://studentsrepo.um.edu.my/5792/1/Molecular_Strategies_For_Control_of_Cymbidium_Mosaic_Virus_Thesis.pdf Dzulkurnain @ Hamidi, Adriya (2013) Molecular strategies for control of cymbidium mosaic virus / Adriya binti Dzulkurnain @ Hamidi. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/5792/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic QH301 Biology
spellingShingle QH301 Biology
Dzulkurnain @ Hamidi, Adriya
Molecular strategies for control of cymbidium mosaic virus / Adriya binti Dzulkurnain @ Hamidi
description Orchid, amongst the most popular flowers in the world, play a major role in the economy of many Asian countries. However, detrimental effects by various viruses can reduce the economic value of this horticultural crop. There have been a number of viruses known to occur in orchids globally with one of the most common being Cymbidium mosaic virus (CymMV). Plant genetic engineering approaches have been successful in producing crops with viral resistance, however there has been a concern expressed that resistance resulting from single gene constructs might be rather narrow (specific to only certain virus sub-type) could easily break down. Thus this research aimed to develop a multiple strategy approach towards the control of CymMV in orchids by combining the concepts of pathogen derived resistance and antibody mediated resistance. A plant transformation vector, pCAMBIA 1304-CymMV-fwd was constructed to contain coat protein sequences from the CymMV. The construct was successfully transformed into explant samples and RT-PCR analysis showed that the pathogen derived resistance sequence was successfully expressed in plants. In a second part to the study anti-CymMV was isolated from a Toxoplasma gondii-immunized single-chain variable fragment (ScFv) antibody phage-display library. Four antibodies were isolated and analyzed through BLASTX and nucleotide BLAST but only one provided significant similarity to PDPK1 3-phosphoinositide dependent protein kinase-1 [Homo sapiens]. The two genes have the potential to be pyramided together in a single plant transformation vector, or to be introduced into plants by successive transformation, in order to develop orchids with longer term viral resistance.
format Thesis
author Dzulkurnain @ Hamidi, Adriya
author_facet Dzulkurnain @ Hamidi, Adriya
author_sort Dzulkurnain @ Hamidi, Adriya
title Molecular strategies for control of cymbidium mosaic virus / Adriya binti Dzulkurnain @ Hamidi
title_short Molecular strategies for control of cymbidium mosaic virus / Adriya binti Dzulkurnain @ Hamidi
title_full Molecular strategies for control of cymbidium mosaic virus / Adriya binti Dzulkurnain @ Hamidi
title_fullStr Molecular strategies for control of cymbidium mosaic virus / Adriya binti Dzulkurnain @ Hamidi
title_full_unstemmed Molecular strategies for control of cymbidium mosaic virus / Adriya binti Dzulkurnain @ Hamidi
title_sort molecular strategies for control of cymbidium mosaic virus / adriya binti dzulkurnain @ hamidi
publishDate 2013
url http://studentsrepo.um.edu.my/5792/3/Name.pdf
http://studentsrepo.um.edu.my/5792/2/Preface.pdf
http://studentsrepo.um.edu.my/5792/1/Molecular_Strategies_For_Control_of_Cymbidium_Mosaic_Virus_Thesis.pdf
http://studentsrepo.um.edu.my/5792/
_version_ 1738505834748444672
score 13.211869

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