Cover Image

Overexpression of wild-type ANXA7 tumor suppressor gene alters cancer-related micrornas in human prostate cancer cells / Yap Lim Hui

MicroRNAs (miRNAs) are endogenous 18-25nt RNAs that regulate genes at the protein translation level. They can act as tumor suppressors or oncogene and are often dysregulated in various cancers. ANXA7 is a tumor suppressor gene that encodes for a Ca2+-dependent membrane-binding protein and its expres...

Full description

Saved in:
Bibliographic Details
Main Author: Yap, Lim Hui
Format: Thesis
Published: 2013
Subjects:
Q Science (General)
Online Access:http://studentsrepo.um.edu.my/4874/1/YAP_LIM_HUI_(SGR090094).pdf
http://studentsrepo.um.edu.my/4874/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.stud.4874
record_format eprints
spelling my.um.stud.48742015-03-06T05:17:31Z Overexpression of wild-type ANXA7 tumor suppressor gene alters cancer-related micrornas in human prostate cancer cells / Yap Lim Hui Yap, Lim Hui Q Science (General) MicroRNAs (miRNAs) are endogenous 18-25nt RNAs that regulate genes at the protein translation level. They can act as tumor suppressors or oncogene and are often dysregulated in various cancers. ANXA7 is a tumor suppressor gene that encodes for a Ca2+-dependent membrane-binding protein and its expression was found to be reduced or lost in advanced androgen-independent prostate cancers. This research aims to identify miRNAs that are regulated by the ANXA7 tumor suppressor gene and the putative pathways involved in prostate cancer. The overexpression of wild-type ANXA7 was achieved by using a mammalian expression vector harboring the gene. From our miRNA microarray expression, a total of 16 miRNAs were found to be significantly differentially expressed in response to increased ANXA7. These include hsa-miR-874, hsa-miR-1284, hsa-miR-543 and hsa-miR-409-5p. All of these miRNAs have predicted targets that are involved in calcium signaling. In conclusion, the altered miRNA expression induced upon increased expression of ANXA7 suggests that miRNAs and the calcium signaling pathways are regulated by ANXA7 and could be manipulated for therapeutic purposes. 2013 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/4874/1/YAP_LIM_HUI_(SGR090094).pdf Yap, Lim Hui (2013) Overexpression of wild-type ANXA7 tumor suppressor gene alters cancer-related micrornas in human prostate cancer cells / Yap Lim Hui. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/4874/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic Q Science (General)
spellingShingle Q Science (General)
Yap, Lim Hui
Overexpression of wild-type ANXA7 tumor suppressor gene alters cancer-related micrornas in human prostate cancer cells / Yap Lim Hui
description MicroRNAs (miRNAs) are endogenous 18-25nt RNAs that regulate genes at the protein translation level. They can act as tumor suppressors or oncogene and are often dysregulated in various cancers. ANXA7 is a tumor suppressor gene that encodes for a Ca2+-dependent membrane-binding protein and its expression was found to be reduced or lost in advanced androgen-independent prostate cancers. This research aims to identify miRNAs that are regulated by the ANXA7 tumor suppressor gene and the putative pathways involved in prostate cancer. The overexpression of wild-type ANXA7 was achieved by using a mammalian expression vector harboring the gene. From our miRNA microarray expression, a total of 16 miRNAs were found to be significantly differentially expressed in response to increased ANXA7. These include hsa-miR-874, hsa-miR-1284, hsa-miR-543 and hsa-miR-409-5p. All of these miRNAs have predicted targets that are involved in calcium signaling. In conclusion, the altered miRNA expression induced upon increased expression of ANXA7 suggests that miRNAs and the calcium signaling pathways are regulated by ANXA7 and could be manipulated for therapeutic purposes.
format Thesis
author Yap, Lim Hui
author_facet Yap, Lim Hui
author_sort Yap, Lim Hui
title Overexpression of wild-type ANXA7 tumor suppressor gene alters cancer-related micrornas in human prostate cancer cells / Yap Lim Hui
title_short Overexpression of wild-type ANXA7 tumor suppressor gene alters cancer-related micrornas in human prostate cancer cells / Yap Lim Hui
title_full Overexpression of wild-type ANXA7 tumor suppressor gene alters cancer-related micrornas in human prostate cancer cells / Yap Lim Hui
title_fullStr Overexpression of wild-type ANXA7 tumor suppressor gene alters cancer-related micrornas in human prostate cancer cells / Yap Lim Hui
title_full_unstemmed Overexpression of wild-type ANXA7 tumor suppressor gene alters cancer-related micrornas in human prostate cancer cells / Yap Lim Hui
title_sort overexpression of wild-type anxa7 tumor suppressor gene alters cancer-related micrornas in human prostate cancer cells / yap lim hui
publishDate 2013
url http://studentsrepo.um.edu.my/4874/1/YAP_LIM_HUI_(SGR090094).pdf
http://studentsrepo.um.edu.my/4874/
_version_ 1738505721942638592
score 13.211869

Similar Items