Determination of alcohol in postmortem blood samples using automated headspace gas chromatography – mass spectrometry / Saravanakumar Maniam
Determination of ethanol in post mortem blood has become one of the important tool in medico legal investigation. The concentration of the ethanol upon dead in a person or alive has legal complication if it is more than permitted level. In Malaysia the level of Blood Alcohol Concentration (BAC) perm...
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| Format: | Thesis |
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2014
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| Online Access: | http://studentsrepo.um.edu.my/4821/1/Saravanakumar_Research_Report_Part_1.pdf http://studentsrepo.um.edu.my/4821/2/Saravanakumar_Research_Report_Part_2.pdf http://studentsrepo.um.edu.my/4821/ |
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| Summary: | Determination of ethanol in post mortem blood has become one of the important tool in medico legal investigation. The concentration of the ethanol upon dead in a person or alive has legal complication if it is more than permitted level. In Malaysia the level of Blood Alcohol Concentration (BAC) permittable according Road Traffic Act 1987 is 0.08g/100ml of blood. Nonetheless the determination of ethanol concentration in fresh blood obtained from alcohol consumer post no significant problem whereas when the blood is obtained from dead person or post-mortem blood it validity is in questioned. Accurate interpretation of blood ethanol concentration at the time of death is a difficult task to obtain due to time and other environmental factor. A reliable and rapid method is required to overcome the difficultness of the analysis. Gas Chromatography Mass Spectrometry assisted with Headspace sampling has given a new way for accurate and rapid analysis with simple procedure. Validation on the method of determination concentration of ethanol in post-mortem blood using iso-propanol as the internal standard was conducted. The validation parameter obtained indicate that iso-propanol is suitable to be used as internal standard. The validated protocol was followed to analysis 50 post-mortem blood sample obtain from Forensic Medicine Department upon autopsy. Result obtained relates that the quantification of ethanol in post-mortem blood can be conducted with high accuracy and specificity. |
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