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Determination of simazine in fish and shrimp samples using ultra performance liquid chromatography tandem mass spectrometer (uplc-ms/ms) / Amirul Ashraf Bin Jamaluddin

Ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was successfully developed and validated for the analysis of simazine in fish and shrimp samples at trace levels to meet European Union requirements and import requirements from certain countries. This method also u...

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Main Author: Amirul Ashraf, Jamaluddin
Format: Thesis
Published: 2014
Subjects:
Q Science (General)
Online Access:http://studentsrepo.um.edu.my/4789/1/Determination_of_Simazine_in_fish_and_shrimp_samples_using_U.pdf
http://studentsrepo.um.edu.my/4789/
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spelling my.um.stud.47892017-11-30T08:41:27Z Determination of simazine in fish and shrimp samples using ultra performance liquid chromatography tandem mass spectrometer (uplc-ms/ms) / Amirul Ashraf Bin Jamaluddin Amirul Ashraf, Jamaluddin Q Science (General) Ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was successfully developed and validated for the analysis of simazine in fish and shrimp samples at trace levels to meet European Union requirements and import requirements from certain countries. This method also utilized a simple, fast and inexpensive extraction technique called QuECheRS (quick, easy, cheap, rugged and safe). Matrix-matched calibration standard are used to construct a calibration curve and Ethoprophos is used as internal standard. At optimal conditions, the chromatographic separation was achieved in less than 10 minutes with the total run time of 13.5 minutes. The mean recoveries for fish matrix ranged from 101 % to 107 % while shrimp matrix shows the range from 97 % to 101 %. Limit of detection (LOD) for simazine in fish and shrimp were 1.18 ng/g and 1.06 ng/g respectively. The limits of quantification (LOQ) were reported as 3.52 ng/g in fish matrix and 3.19 ng/g in shrimp matrix. Results from this study showed that the concentration of simazine obtained in fish and shrimp samples were below the limit of quantification and it is reported as not detected in the sample. 2014 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/4789/1/Determination_of_Simazine_in_fish_and_shrimp_samples_using_U.pdf Amirul Ashraf, Jamaluddin (2014) Determination of simazine in fish and shrimp samples using ultra performance liquid chromatography tandem mass spectrometer (uplc-ms/ms) / Amirul Ashraf Bin Jamaluddin. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/4789/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic Q Science (General)
spellingShingle Q Science (General)
Amirul Ashraf, Jamaluddin
Determination of simazine in fish and shrimp samples using ultra performance liquid chromatography tandem mass spectrometer (uplc-ms/ms) / Amirul Ashraf Bin Jamaluddin
description Ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was successfully developed and validated for the analysis of simazine in fish and shrimp samples at trace levels to meet European Union requirements and import requirements from certain countries. This method also utilized a simple, fast and inexpensive extraction technique called QuECheRS (quick, easy, cheap, rugged and safe). Matrix-matched calibration standard are used to construct a calibration curve and Ethoprophos is used as internal standard. At optimal conditions, the chromatographic separation was achieved in less than 10 minutes with the total run time of 13.5 minutes. The mean recoveries for fish matrix ranged from 101 % to 107 % while shrimp matrix shows the range from 97 % to 101 %. Limit of detection (LOD) for simazine in fish and shrimp were 1.18 ng/g and 1.06 ng/g respectively. The limits of quantification (LOQ) were reported as 3.52 ng/g in fish matrix and 3.19 ng/g in shrimp matrix. Results from this study showed that the concentration of simazine obtained in fish and shrimp samples were below the limit of quantification and it is reported as not detected in the sample.
format Thesis
author Amirul Ashraf, Jamaluddin
author_facet Amirul Ashraf, Jamaluddin
author_sort Amirul Ashraf, Jamaluddin
title Determination of simazine in fish and shrimp samples using ultra performance liquid chromatography tandem mass spectrometer (uplc-ms/ms) / Amirul Ashraf Bin Jamaluddin
title_short Determination of simazine in fish and shrimp samples using ultra performance liquid chromatography tandem mass spectrometer (uplc-ms/ms) / Amirul Ashraf Bin Jamaluddin
title_full Determination of simazine in fish and shrimp samples using ultra performance liquid chromatography tandem mass spectrometer (uplc-ms/ms) / Amirul Ashraf Bin Jamaluddin
title_fullStr Determination of simazine in fish and shrimp samples using ultra performance liquid chromatography tandem mass spectrometer (uplc-ms/ms) / Amirul Ashraf Bin Jamaluddin
title_full_unstemmed Determination of simazine in fish and shrimp samples using ultra performance liquid chromatography tandem mass spectrometer (uplc-ms/ms) / Amirul Ashraf Bin Jamaluddin
title_sort determination of simazine in fish and shrimp samples using ultra performance liquid chromatography tandem mass spectrometer (uplc-ms/ms) / amirul ashraf bin jamaluddin
publishDate 2014
url http://studentsrepo.um.edu.my/4789/1/Determination_of_Simazine_in_fish_and_shrimp_samples_using_U.pdf
http://studentsrepo.um.edu.my/4789/
_version_ 1738505711656108032
score 13.211869

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