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Genetic polymorphisms of the NOD1,CXCL16,STAT6,TLR4, ATG16LI and IRGM Genesin Malaysia patients with crohn's disease / Ng Jin Guan

Crohn’s disease (CD) is one of the major type of inflammatory bowel diseases (IBD) that affects the gastrointestinal tract. There were a lot of susceptible genes being published based on different population studies as well as meta-analysis projects. In this study, we aimed to investigate the ass...

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Main Author: Ng, Jin Guan
Format: Thesis
Published: 2014
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Q Science (General)
Online Access:http://studentsrepo.um.edu.my/4778/1/SGF110024_Thesis_Part_1.pdf
http://studentsrepo.um.edu.my/4778/2/SGF110024_Thesis_Part_2.pdf
http://studentsrepo.um.edu.my/4778/
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spelling my.um.stud.47782015-03-12T05:00:38Z Genetic polymorphisms of the NOD1,CXCL16,STAT6,TLR4, ATG16LI and IRGM Genesin Malaysia patients with crohn's disease / Ng Jin Guan Ng, Jin Guan Q Science (General) Crohn’s disease (CD) is one of the major type of inflammatory bowel diseases (IBD) that affects the gastrointestinal tract. There were a lot of susceptible genes being published based on different population studies as well as meta-analysis projects. In this study, we aimed to investigate the association of some genes, NOD1, CXCL16, STAT6, TLR4, ATG16L1 and IRGM had been found with association to the disease on non- Malaysian population. Overall, 85 CD DNA samples and 250 control healthy samples were collected from University Malaya Medical Center (UMMC). DNA were extracted and analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) or TaqMan assay amplification. Results obtained showed that NOD1 (PG/A=0.5179, ORG/A=1.135, 95% CIG/A=0.786 – 1.637), STAT6 (PA/G=0.531, ORA/G=0.884, 95% CIA/G=0.621 - 1.258) and IRGM variants (Prs4958847=0.154, Prs11747270=0.09359, Prs72553867=0.8844) had no association with CD. CXCL16 (PC/T=0.04819, ORC/T=1.431, 95% CIC/T=1.009 - 2.031), TLR4 (PC/T=0.007, ORC/T=0.437, 95% CIC/T=0.242 - 0.789) and ATG16L1 variant rs2241880 (PA/G=0.01462, ORA/G=0.645, 95% CIA/G =0.454 - 0.917) were found to be associated with Malaysian patients. For stratified analysis, TLR4 (P=0.0002399) and ATG16L1 rs2241880 variant (P=0.03619) were significantly associated with Malay while ATG16L1 variant 6754677 (P=6.3x10-3) was associated with Indian patients. In conclusion, CXCL16, TLR4 and ATG16L1 but not NOD1, STAT6 and IRGM were found to have strong association with CD in the Malaysian population. 2014 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/4778/1/SGF110024_Thesis_Part_1.pdf application/pdf http://studentsrepo.um.edu.my/4778/2/SGF110024_Thesis_Part_2.pdf Ng, Jin Guan (2014) Genetic polymorphisms of the NOD1,CXCL16,STAT6,TLR4, ATG16LI and IRGM Genesin Malaysia patients with crohn's disease / Ng Jin Guan. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/4778/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic Q Science (General)
spellingShingle Q Science (General)
Ng, Jin Guan
Genetic polymorphisms of the NOD1,CXCL16,STAT6,TLR4, ATG16LI and IRGM Genesin Malaysia patients with crohn's disease / Ng Jin Guan
description Crohn’s disease (CD) is one of the major type of inflammatory bowel diseases (IBD) that affects the gastrointestinal tract. There were a lot of susceptible genes being published based on different population studies as well as meta-analysis projects. In this study, we aimed to investigate the association of some genes, NOD1, CXCL16, STAT6, TLR4, ATG16L1 and IRGM had been found with association to the disease on non- Malaysian population. Overall, 85 CD DNA samples and 250 control healthy samples were collected from University Malaya Medical Center (UMMC). DNA were extracted and analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) or TaqMan assay amplification. Results obtained showed that NOD1 (PG/A=0.5179, ORG/A=1.135, 95% CIG/A=0.786 – 1.637), STAT6 (PA/G=0.531, ORA/G=0.884, 95% CIA/G=0.621 - 1.258) and IRGM variants (Prs4958847=0.154, Prs11747270=0.09359, Prs72553867=0.8844) had no association with CD. CXCL16 (PC/T=0.04819, ORC/T=1.431, 95% CIC/T=1.009 - 2.031), TLR4 (PC/T=0.007, ORC/T=0.437, 95% CIC/T=0.242 - 0.789) and ATG16L1 variant rs2241880 (PA/G=0.01462, ORA/G=0.645, 95% CIA/G =0.454 - 0.917) were found to be associated with Malaysian patients. For stratified analysis, TLR4 (P=0.0002399) and ATG16L1 rs2241880 variant (P=0.03619) were significantly associated with Malay while ATG16L1 variant 6754677 (P=6.3x10-3) was associated with Indian patients. In conclusion, CXCL16, TLR4 and ATG16L1 but not NOD1, STAT6 and IRGM were found to have strong association with CD in the Malaysian population.
format Thesis
author Ng, Jin Guan
author_facet Ng, Jin Guan
author_sort Ng, Jin Guan
title Genetic polymorphisms of the NOD1,CXCL16,STAT6,TLR4, ATG16LI and IRGM Genesin Malaysia patients with crohn's disease / Ng Jin Guan
title_short Genetic polymorphisms of the NOD1,CXCL16,STAT6,TLR4, ATG16LI and IRGM Genesin Malaysia patients with crohn's disease / Ng Jin Guan
title_full Genetic polymorphisms of the NOD1,CXCL16,STAT6,TLR4, ATG16LI and IRGM Genesin Malaysia patients with crohn's disease / Ng Jin Guan
title_fullStr Genetic polymorphisms of the NOD1,CXCL16,STAT6,TLR4, ATG16LI and IRGM Genesin Malaysia patients with crohn's disease / Ng Jin Guan
title_full_unstemmed Genetic polymorphisms of the NOD1,CXCL16,STAT6,TLR4, ATG16LI and IRGM Genesin Malaysia patients with crohn's disease / Ng Jin Guan
title_sort genetic polymorphisms of the nod1,cxcl16,stat6,tlr4, atg16li and irgm genesin malaysia patients with crohn's disease / ng jin guan
publishDate 2014
url http://studentsrepo.um.edu.my/4778/1/SGF110024_Thesis_Part_1.pdf
http://studentsrepo.um.edu.my/4778/2/SGF110024_Thesis_Part_2.pdf
http://studentsrepo.um.edu.my/4778/
_version_ 1738505710336999424
score 13.211869

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