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The application of irap markers in the breeding of papaya / Bathusha Shareef bin Kader Shaik Ali

In Malaysia, a papaya breeding program was initiated by the Malaysian Agricultural Research and Development Institute (MARDI) to produce resistant papayas. Nevertheless, selection of resistant papayas from field trials is laborious and time-consuming. In order to facilitate selection of resistant pa...

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Main Author: Kader Shaik Ali, Bathusha Shareef
Format: Thesis
Published: 2013
Subjects:
TP Chemical technology
Online Access:http://studentsrepo.um.edu.my/4364/1/Bathusha_MSc_final.pdf
http://studentsrepo.um.edu.my/4364/
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spelling my.um.stud.43642014-09-30T05:14:14Z The application of irap markers in the breeding of papaya / Bathusha Shareef bin Kader Shaik Ali Kader Shaik Ali, Bathusha Shareef TP Chemical technology In Malaysia, a papaya breeding program was initiated by the Malaysian Agricultural Research and Development Institute (MARDI) to produce resistant papayas. Nevertheless, selection of resistant papayas from field trials is laborious and time-consuming. In order to facilitate selection of resistant papayas in breeding programs, potential of DNA fingerprinting method was analysed in this study for future applications. Materials from leaves of Carica papaya L. from varieties Tainung 5, Eksotika 6 and their hybrids were used in the assessment of molecular markers. From an analysis of Inter-Retrotransposons Amplified Polymorphism Reaction (IRAP) molecular markers, five combinations out of 45 possible primers combinations showed significant polymorphism in DNA banding patterns. Furthermore, the banding patterns were used to assess the phylogenetic relationship between the papayas sample tested. Out of the five combinations, only one combination of primers showed DNA banding patterns which is similar to the phenotypic segregation of MARDI’s field trial results. IRAP markers could be further tested to be applied in Molecular Assisted Selection (MAS) and Molecular Assisted Breeding (MAB) to facilitate selection of PRSV resistant papayas. For future studies, more IRAP primers can be designed based on Long Terminal Repeat (LTR) sequences of the papaya genome. IRAP markers could also be used in combination with other primer sequences such as from microsatellites. (Retrotransposon-microsatellite Amplified Polymorphism). This is to obtain more informative banding patterns and thus IRAP markers could be fully exploited to facilitate papaya breeding programmes. Hopefully, this would benefit the papaya planting industry. 2013 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/4364/1/Bathusha_MSc_final.pdf Kader Shaik Ali, Bathusha Shareef (2013) The application of irap markers in the breeding of papaya / Bathusha Shareef bin Kader Shaik Ali. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/4364/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Kader Shaik Ali, Bathusha Shareef
The application of irap markers in the breeding of papaya / Bathusha Shareef bin Kader Shaik Ali
description In Malaysia, a papaya breeding program was initiated by the Malaysian Agricultural Research and Development Institute (MARDI) to produce resistant papayas. Nevertheless, selection of resistant papayas from field trials is laborious and time-consuming. In order to facilitate selection of resistant papayas in breeding programs, potential of DNA fingerprinting method was analysed in this study for future applications. Materials from leaves of Carica papaya L. from varieties Tainung 5, Eksotika 6 and their hybrids were used in the assessment of molecular markers. From an analysis of Inter-Retrotransposons Amplified Polymorphism Reaction (IRAP) molecular markers, five combinations out of 45 possible primers combinations showed significant polymorphism in DNA banding patterns. Furthermore, the banding patterns were used to assess the phylogenetic relationship between the papayas sample tested. Out of the five combinations, only one combination of primers showed DNA banding patterns which is similar to the phenotypic segregation of MARDI’s field trial results. IRAP markers could be further tested to be applied in Molecular Assisted Selection (MAS) and Molecular Assisted Breeding (MAB) to facilitate selection of PRSV resistant papayas. For future studies, more IRAP primers can be designed based on Long Terminal Repeat (LTR) sequences of the papaya genome. IRAP markers could also be used in combination with other primer sequences such as from microsatellites. (Retrotransposon-microsatellite Amplified Polymorphism). This is to obtain more informative banding patterns and thus IRAP markers could be fully exploited to facilitate papaya breeding programmes. Hopefully, this would benefit the papaya planting industry.
format Thesis
author Kader Shaik Ali, Bathusha Shareef
author_facet Kader Shaik Ali, Bathusha Shareef
author_sort Kader Shaik Ali, Bathusha Shareef
title The application of irap markers in the breeding of papaya / Bathusha Shareef bin Kader Shaik Ali
title_short The application of irap markers in the breeding of papaya / Bathusha Shareef bin Kader Shaik Ali
title_full The application of irap markers in the breeding of papaya / Bathusha Shareef bin Kader Shaik Ali
title_fullStr The application of irap markers in the breeding of papaya / Bathusha Shareef bin Kader Shaik Ali
title_full_unstemmed The application of irap markers in the breeding of papaya / Bathusha Shareef bin Kader Shaik Ali
title_sort application of irap markers in the breeding of papaya / bathusha shareef bin kader shaik ali
publishDate 2013
url http://studentsrepo.um.edu.my/4364/1/Bathusha_MSc_final.pdf
http://studentsrepo.um.edu.my/4364/
_version_ 1738505669591433216
score 13.211869

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