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Isolation and toxin typing of clostridium perfringens from Sungai Selangor and Sungai Bernam / Florence Lee Chi Hiong

Clostridium perfringens (CP) is an anaerobic, spore forming bacterium that is emerging as a preferred faecal pollution indicator. In the local scene, little is known about the density and toxinotype of CP in river water, and how does river discharge affects the density. Therefore, water samples wer...

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Main Author:	Lee, Florence Chi Hiong
Format:	Thesis
Published:	2009
Subjects:	Q Science (General) QH Natural history
Online Access:	http://studentsrepo.um.edu.my/4288/1/Abstrak.pdf http://studentsrepo.um.edu.my/4288/5/Chapter_One_Introduction.pdf http://studentsrepo.um.edu.my/4288/7/Chapter_Two_Literature_Review.pdf http://studentsrepo.um.edu.my/4288/6/Chapter_Three_Materials_and_Methods.pdf http://studentsrepo.um.edu.my/4288/4/Chapter_Four_Results.pdf http://studentsrepo.um.edu.my/4288/3/Chapter_Five_and_Six.pdf http://studentsrepo.um.edu.my/4288/2/APPENDIX.pdf http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f796$002fSD_ILS:796833/one?qu=Isolation+and+toxin+typing+of+clostridium+perfringens+from+Sungai+Selangor http://studentsrepo.um.edu.my/4288/
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spelling	my.um.stud.42882014-09-25T06:00:54Z Isolation and toxin typing of clostridium perfringens from Sungai Selangor and Sungai Bernam / Florence Lee Chi Hiong Lee, Florence Chi Hiong Q Science (General) QH Natural history Clostridium perfringens (CP) is an anaerobic, spore forming bacterium that is emerging as a preferred faecal pollution indicator. In the local scene, little is known about the density and toxinotype of CP in river water, and how does river discharge affects the density. Therefore, water samples were taken from three Malaysian rivers, Sungai Bernam, Sungai Selangor and Tengi Canal between April 2007 and January 2008 to examine the CP densities and toxinotypes. The rivers were selected because of their differences in adjacent land usage. Sungai Selangor reported lower CP isolation rate ranging between 0 to 25% but higher CP densities of <1 to 2695 cfu/100ml. In contrast, CP isolation rates in Sungai Bernam were higher at 0 to 35% but the densities were lower at <1 to 763 cfu/100ml. Tengi Canal showed the lowest CP isolation rate of 0 to 10% and CP densities of <1 to 212 cfu/100ml. Sulphite reducing Clostridia (SRC) were consistently present, and the densities were significantly different temporally in each of the study sites. The highest CP and SRC densities were in the downstream of Sungai Selangor. No significant correlation was found between CP densities and river discharge in all the study sites. Toxinotyping was performed with Polymerase Chain Reaction (PCR) using published primer sequences, and multiplex primers designed specifically to include also the detection of enterotoxin (CPE) gene. All 142 CP isolates found in this study belonged to Clostridium perfringens Type A. Five of the isolates also harboured CPE gene. 2009 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/4288/1/Abstrak.pdf application/pdf http://studentsrepo.um.edu.my/4288/5/Chapter_One_Introduction.pdf application/pdf http://studentsrepo.um.edu.my/4288/7/Chapter_Two_Literature_Review.pdf application/pdf http://studentsrepo.um.edu.my/4288/6/Chapter_Three_Materials_and_Methods.pdf application/pdf http://studentsrepo.um.edu.my/4288/4/Chapter_Four_Results.pdf application/pdf http://studentsrepo.um.edu.my/4288/3/Chapter_Five_and_Six.pdf application/pdf http://studentsrepo.um.edu.my/4288/2/APPENDIX.pdf http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f796$002fSD_ILS:796833/one?qu=Isolation+and+toxin+typing+of+clostridium+perfringens+from+Sungai+Selangor Lee, Florence Chi Hiong (2009) Isolation and toxin typing of clostridium perfringens from Sungai Selangor and Sungai Bernam / Florence Lee Chi Hiong. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/4288/
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Student Repository
url_provider	http://studentsrepo.um.edu.my/
topic	Q Science (General) QH Natural history
spellingShingle	Q Science (General) QH Natural history Lee, Florence Chi Hiong Isolation and toxin typing of clostridium perfringens from Sungai Selangor and Sungai Bernam / Florence Lee Chi Hiong
description	Clostridium perfringens (CP) is an anaerobic, spore forming bacterium that is emerging as a preferred faecal pollution indicator. In the local scene, little is known about the density and toxinotype of CP in river water, and how does river discharge affects the density. Therefore, water samples were taken from three Malaysian rivers, Sungai Bernam, Sungai Selangor and Tengi Canal between April 2007 and January 2008 to examine the CP densities and toxinotypes. The rivers were selected because of their differences in adjacent land usage. Sungai Selangor reported lower CP isolation rate ranging between 0 to 25% but higher CP densities of <1 to 2695 cfu/100ml. In contrast, CP isolation rates in Sungai Bernam were higher at 0 to 35% but the densities were lower at <1 to 763 cfu/100ml. Tengi Canal showed the lowest CP isolation rate of 0 to 10% and CP densities of <1 to 212 cfu/100ml. Sulphite reducing Clostridia (SRC) were consistently present, and the densities were significantly different temporally in each of the study sites. The highest CP and SRC densities were in the downstream of Sungai Selangor. No significant correlation was found between CP densities and river discharge in all the study sites. Toxinotyping was performed with Polymerase Chain Reaction (PCR) using published primer sequences, and multiplex primers designed specifically to include also the detection of enterotoxin (CPE) gene. All 142 CP isolates found in this study belonged to Clostridium perfringens Type A. Five of the isolates also harboured CPE gene.
format	Thesis
author	Lee, Florence Chi Hiong
author_facet	Lee, Florence Chi Hiong
author_sort	Lee, Florence Chi Hiong
title	Isolation and toxin typing of clostridium perfringens from Sungai Selangor and Sungai Bernam / Florence Lee Chi Hiong
title_short	Isolation and toxin typing of clostridium perfringens from Sungai Selangor and Sungai Bernam / Florence Lee Chi Hiong
title_full	Isolation and toxin typing of clostridium perfringens from Sungai Selangor and Sungai Bernam / Florence Lee Chi Hiong
title_fullStr	Isolation and toxin typing of clostridium perfringens from Sungai Selangor and Sungai Bernam / Florence Lee Chi Hiong
title_full_unstemmed	Isolation and toxin typing of clostridium perfringens from Sungai Selangor and Sungai Bernam / Florence Lee Chi Hiong
title_sort	isolation and toxin typing of clostridium perfringens from sungai selangor and sungai bernam / florence lee chi hiong
publishDate	2009
url	http://studentsrepo.um.edu.my/4288/1/Abstrak.pdf http://studentsrepo.um.edu.my/4288/5/Chapter_One_Introduction.pdf http://studentsrepo.um.edu.my/4288/7/Chapter_Two_Literature_Review.pdf http://studentsrepo.um.edu.my/4288/6/Chapter_Three_Materials_and_Methods.pdf http://studentsrepo.um.edu.my/4288/4/Chapter_Four_Results.pdf http://studentsrepo.um.edu.my/4288/3/Chapter_Five_and_Six.pdf http://studentsrepo.um.edu.my/4288/2/APPENDIX.pdf http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f796$002fSD_ILS:796833/one?qu=Isolation+and+toxin+typing+of+clostridium+perfringens+from+Sungai+Selangor http://studentsrepo.um.edu.my/4288/
_version_	1738505659920416768
score	13.211869

Isolation and toxin typing of clostridium perfringens from Sungai Selangor and Sungai Bernam / Florence Lee Chi Hiong

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