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Computational identification of micro RNA in MUSA sp EST sequences / Tan Yew Seong

MicroRNAs (miRNAs) are small single-stranded non-protein-coding RNAs of about 22 nucleotides that play an important role in post-transcriptional gene regulation in animals and plants by targeting mRNAs for direct cleavage of mRNAs or repression of mRNA translation. In this study, a homology search b...

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Main Author: Tan, Yew Seong
Format: Thesis
Published: 2010
Subjects:
TP Chemical technology
Online Access:http://studentsrepo.um.edu.my/4281/1/0_Front_cover.pdf
http://studentsrepo.um.edu.my/4281/2/1_Title_page.pdf
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spelling my.um.stud.42812014-09-25T02:41:59Z Computational identification of micro RNA in MUSA sp EST sequences / Tan Yew Seong Tan, Yew Seong TP Chemical technology MicroRNAs (miRNAs) are small single-stranded non-protein-coding RNAs of about 22 nucleotides that play an important role in post-transcriptional gene regulation in animals and plants by targeting mRNAs for direct cleavage of mRNAs or repression of mRNA translation. In this study, a homology search based approach has been used for identifying the novel miRNA genes of Musa sp. using the BLAST program with a total of 42,978 Musa sp. expressed sequence tags (EST) were compared to a total of 959 previously known plant miRNA sequences. Forty-seven candidate miRNA with homology to previously known miRNAs were identified. By using the mFold3.1 program, secondary RNA structures were predicted and total of nine novel miRNA were identified in Musa sp. One of the most promising novel miRNA was miR156 which has the strongest characteristics of a miRNA precursor structure and was 100% identical to the mature miR156 from other plant species. It was experimentally validated to be expressed in Musa acuminata var. Berangan and is the first known report of the identification and expression of miRNA from a banana plant species. 2010 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/4281/1/0_Front_cover.pdf application/pdf http://studentsrepo.um.edu.my/4281/2/1_Title_page.pdf application/pdf http://studentsrepo.um.edu.my/4281/3/2_A_ORIGINAL_LITERARY_WORK_DECLARATION.pdf application/pdf http://studentsrepo.um.edu.my/4281/4/3_Full_thesis.pdf http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f796$002fSD_ILS:796682/one?qu=Computational+identification+of+micro+rna+in+musa Tan, Yew Seong (2010) Computational identification of micro RNA in MUSA sp EST sequences / Tan Yew Seong. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/4281/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Tan, Yew Seong
Computational identification of micro RNA in MUSA sp EST sequences / Tan Yew Seong
description MicroRNAs (miRNAs) are small single-stranded non-protein-coding RNAs of about 22 nucleotides that play an important role in post-transcriptional gene regulation in animals and plants by targeting mRNAs for direct cleavage of mRNAs or repression of mRNA translation. In this study, a homology search based approach has been used for identifying the novel miRNA genes of Musa sp. using the BLAST program with a total of 42,978 Musa sp. expressed sequence tags (EST) were compared to a total of 959 previously known plant miRNA sequences. Forty-seven candidate miRNA with homology to previously known miRNAs were identified. By using the mFold3.1 program, secondary RNA structures were predicted and total of nine novel miRNA were identified in Musa sp. One of the most promising novel miRNA was miR156 which has the strongest characteristics of a miRNA precursor structure and was 100% identical to the mature miR156 from other plant species. It was experimentally validated to be expressed in Musa acuminata var. Berangan and is the first known report of the identification and expression of miRNA from a banana plant species.
format Thesis
author Tan, Yew Seong
author_facet Tan, Yew Seong
author_sort Tan, Yew Seong
title Computational identification of micro RNA in MUSA sp EST sequences / Tan Yew Seong
title_short Computational identification of micro RNA in MUSA sp EST sequences / Tan Yew Seong
title_full Computational identification of micro RNA in MUSA sp EST sequences / Tan Yew Seong
title_fullStr Computational identification of micro RNA in MUSA sp EST sequences / Tan Yew Seong
title_full_unstemmed Computational identification of micro RNA in MUSA sp EST sequences / Tan Yew Seong
title_sort computational identification of micro rna in musa sp est sequences / tan yew seong
publishDate 2010
url http://studentsrepo.um.edu.my/4281/1/0_Front_cover.pdf
http://studentsrepo.um.edu.my/4281/2/1_Title_page.pdf
http://studentsrepo.um.edu.my/4281/3/2_A_ORIGINAL_LITERARY_WORK_DECLARATION.pdf
http://studentsrepo.um.edu.my/4281/4/3_Full_thesis.pdf
http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f796$002fSD_ILS:796682/one?qu=Computational+identification+of+micro+rna+in+musa
http://studentsrepo.um.edu.my/4281/
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score 13.211869

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