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An investigation of the roles of MYB transcription factors in orchid dendrobium hybrida flower development / Lau Su Ee

MYB genes encode a large family of transcription regulators in eukaryotes. Most plant MYB genes belong to the R2R3-MYB subfamily. MYB gene family members have been studied in various plant species and play roles in the regulation of plant development, hormone signalling, defence response and seconda...

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Main Author: Lau, Su Ee
Format: Thesis
Published: 2013
Subjects:
Q Science (General)
QH Natural history
Online Access:http://studentsrepo.um.edu.my/4214/3/LAU_SU_EE_SGR090066.pdf
http://studentsrepo.um.edu.my/4214/1/appendix_3.PDF
http://studentsrepo.um.edu.my/4214/2/appendix_7.PDF
http://studentsrepo.um.edu.my/4214/
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spelling my.um.stud.42142014-10-03T08:38:49Z An investigation of the roles of MYB transcription factors in orchid dendrobium hybrida flower development / Lau Su Ee Lau, Su Ee Q Science (General) QH Natural history MYB genes encode a large family of transcription regulators in eukaryotes. Most plant MYB genes belong to the R2R3-MYB subfamily. MYB gene family members have been studied in various plant species and play roles in the regulation of plant development, hormone signalling, defence response and secondary metabolism. Orchid plants play an important role in the floral industry owing to their unique flower shapes, fragrance, colours and keeping quality. Flower colours are influenced by flower pigmentation, vacuolar compounds, pH, metal ions and shape of epidermal cells. R2R3-MYB genes regulate flower pigmentation, flower shape and cell shape therefore have importance in the development of new varieties of orchids. Polymerase chain reaction with sequence consensus based primer was used to amplify thirty-seven partial cDNA fragments representing R2R3-MYB gene sequences were isolated from Dendrobium hybrida. Phylogenetic analysis with IT3F: An Interspecies Transcription Factor Function Finder for Plants (http://jicbio.bbsrc.ac.uk/IT3F) of the translated R2R3-MYB gene sequences revealed that MYB sequences from Dendrobium hybrida are clustered into six subgroups. Nine of the translated DhMYB genes were clustered in subgroup 9 which are known to be involved in cell shape development. Production of dsRNA, sense and antisense RNAs were synthesized in vitro from the corresponding DNA plasmids by using double T7 RNA polymerase. RNAi knock down of the expression of DhMYB using dsRNA approach showed significant reduction of DhMYB gene expression compared with untreated control. Cell shape scanning electron microscopy of adaxial epidermal cells within domain 1 of the lip of flowers treated with DhMYB dsRNA had flattened epidermal cells whilst those of control flowers were conical – papillate. We suggest that DhMYB cDNA controls cell morphogenesis in the lip epidermis. 2013 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/4214/3/LAU_SU_EE_SGR090066.pdf application/pdf http://studentsrepo.um.edu.my/4214/1/appendix_3.PDF application/pdf http://studentsrepo.um.edu.my/4214/2/appendix_7.PDF Lau, Su Ee (2013) An investigation of the roles of MYB transcription factors in orchid dendrobium hybrida flower development / Lau Su Ee. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/4214/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic Q Science (General)
QH Natural history
spellingShingle Q Science (General)
QH Natural history
Lau, Su Ee
An investigation of the roles of MYB transcription factors in orchid dendrobium hybrida flower development / Lau Su Ee
description MYB genes encode a large family of transcription regulators in eukaryotes. Most plant MYB genes belong to the R2R3-MYB subfamily. MYB gene family members have been studied in various plant species and play roles in the regulation of plant development, hormone signalling, defence response and secondary metabolism. Orchid plants play an important role in the floral industry owing to their unique flower shapes, fragrance, colours and keeping quality. Flower colours are influenced by flower pigmentation, vacuolar compounds, pH, metal ions and shape of epidermal cells. R2R3-MYB genes regulate flower pigmentation, flower shape and cell shape therefore have importance in the development of new varieties of orchids. Polymerase chain reaction with sequence consensus based primer was used to amplify thirty-seven partial cDNA fragments representing R2R3-MYB gene sequences were isolated from Dendrobium hybrida. Phylogenetic analysis with IT3F: An Interspecies Transcription Factor Function Finder for Plants (http://jicbio.bbsrc.ac.uk/IT3F) of the translated R2R3-MYB gene sequences revealed that MYB sequences from Dendrobium hybrida are clustered into six subgroups. Nine of the translated DhMYB genes were clustered in subgroup 9 which are known to be involved in cell shape development. Production of dsRNA, sense and antisense RNAs were synthesized in vitro from the corresponding DNA plasmids by using double T7 RNA polymerase. RNAi knock down of the expression of DhMYB using dsRNA approach showed significant reduction of DhMYB gene expression compared with untreated control. Cell shape scanning electron microscopy of adaxial epidermal cells within domain 1 of the lip of flowers treated with DhMYB dsRNA had flattened epidermal cells whilst those of control flowers were conical – papillate. We suggest that DhMYB cDNA controls cell morphogenesis in the lip epidermis.
format Thesis
author Lau, Su Ee
author_facet Lau, Su Ee
author_sort Lau, Su Ee
title An investigation of the roles of MYB transcription factors in orchid dendrobium hybrida flower development / Lau Su Ee
title_short An investigation of the roles of MYB transcription factors in orchid dendrobium hybrida flower development / Lau Su Ee
title_full An investigation of the roles of MYB transcription factors in orchid dendrobium hybrida flower development / Lau Su Ee
title_fullStr An investigation of the roles of MYB transcription factors in orchid dendrobium hybrida flower development / Lau Su Ee
title_full_unstemmed An investigation of the roles of MYB transcription factors in orchid dendrobium hybrida flower development / Lau Su Ee
title_sort investigation of the roles of myb transcription factors in orchid dendrobium hybrida flower development / lau su ee
publishDate 2013
url http://studentsrepo.um.edu.my/4214/3/LAU_SU_EE_SGR090066.pdf
http://studentsrepo.um.edu.my/4214/1/appendix_3.PDF
http://studentsrepo.um.edu.my/4214/2/appendix_7.PDF
http://studentsrepo.um.edu.my/4214/
_version_ 1738505651566411776
score 13.211869

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