Spinal motor neuronal organisation of selected local freshwater fishes in relation to median fin innervations / Kwong Soke Chee
Fish fins are appendages which represent the limbs in higher vertebrates. In general, fish moves in the water by body undulation with the assistance of fins. To date, most of the studies focused on the innervations of limbs especially in mammals, such as rats, cats and monkeys. On the contrary, u...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Published: |
2012
|
| Subjects: | |
| Online Access: | http://studentsrepo.um.edu.my/3781/1/1._Title_page%2C_abstract%2C_content.pdf http://studentsrepo.um.edu.my/3781/2/2._Chapter_1_%E2%80%93_7.pdf http://studentsrepo.um.edu.my/3781/3/3._References.pdf http://studentsrepo.um.edu.my/3781/4/4._Appendices.pdf http://pendeta.um.edu.my/client/default/search/results?qu=Spinal+motor+neuronal+organisation+of+selected+local+freshwater+fishes+in+relation+to+median+fin+innervations&te= http://studentsrepo.um.edu.my/3781/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my.um.stud.3781 |
|---|---|
| record_format |
eprints |
| institution |
Universiti Malaya |
| building |
UM Library |
| collection |
Institutional Repository |
| continent |
Asia |
| country |
Malaysia |
| content_provider |
Universiti Malaya |
| content_source |
UM Student Repository |
| url_provider |
http://studentsrepo.um.edu.my/ |
| topic |
QH301 Biology |
| spellingShingle |
QH301 Biology Kwong, Soke Chee Spinal motor neuronal organisation of selected local freshwater fishes in relation to median fin innervations / Kwong Soke Chee |
| description |
Fish fins are appendages which represent the limbs in higher vertebrates. In general, fish
moves in the water by body undulation with the assistance of fins. To date, most of the
studies focused on the innervations of limbs especially in mammals, such as rats, cats
and monkeys. On the contrary, understanding on the innervations of the fish fin is still
at its infancy as studies on the relevant field are relatively scarce. Hence, this study
aimed to fill the knowledge gap, i.e. to investigate the organisation of spinal motor
neurons in relation to the distribution of the median fins. For comparison purposes, four
species of juvenile fishes utilised in this study were divided into two groups, namely (i)
fish with long and continuous dorsal fin (Channa micropeltes, toman and Clarias sp.,
keli), and (ii) fish with short and non-continuous dorsal fin (Mystus nemurus, baung and
Pangasius sp., patin). Spinal cord tissue obtained from three different representative
segments along the rostro-caudal axis were processed histologically and stained using
three neurohistological staining techniques including H&E, Nissl as well as Lillie’s
Variant of the Weil-Weigert prior to light microscopy level observation. The
organisation of motor neurons was correlated with the distribution of fins. Cell column
organisation characterised the location of motor neurons into seven cell columns,
namely intermediolateral (IL), dorsomedial (DM), ventromedial (VM), central (C),
ventrolateral (VL), dorsoventral (DV) and retrodorsolateral (RDL) cell columns. It was
observed that median motor neurons (IL, DM and VM cell columns) outnumbered
central (C cell column) and lateral motor neurons (VL, DL and RDL cell columns) at all
rostral, median, and caudal region of the fish body trunk. Medial motor neurons (IL and
DM cell columns) were found in all three segments; thus, were probably innervating the
body trunk, for instance to aid in body undulation. On the contrary, DL motor neurons
were found only at the dorsal fin area of Clarias sp. and Pangasius sp.; thus, were probably innervating the particular fin. To further investigate the innervations of caudal
fin muscles through HRP histochemistry, Clarias sp. with rounded caudal fin and
Pangasius sp. with forked caudal fin were selected from each group. The nerve trunks at
the caudal fin were transected and soaked in 50% HRP solution. After two days, each
fish was sacrificed and its spinal cord was freeze-sectioned using cryostat. The sections
were stained using DAB Liquid Enhanced Substrate kit to reveal the motor neurons
containing HRP in the cytoplasm which appeared as brownish precipitations. Most of
the motor neurons innervating Clarias sp. rounded caudal fin were located medially at
IL, DM and VM cell columns. In contrast, the motor neurons innervating forked caudal
fin of Pangasius sp. were located mostly at the ventral areas, namely VM and VL cell
columns. The differences of the motor neuronal organisation between the two species
could be credited to further muscle differentiation in the caudal fin muscle layers of
Pangasius sp., which exhibited more complicated organisation than that of the Clarias
sp. In conclusion, the findings of this study were in agreement with the existing
literature that medial motor neurons (IL and DM cell columns) innervate body trunk
while more laterally located motor neurons (VM, VL and DL cell columns) innervate
distal structure, in this case the fins. Future research can focus, specifically, on the
innervations by individual nerve trunk or innervations of individual muscle and/or the
research can be conducted at different stages of life cycle of the fish. |
| format |
Thesis |
| author |
Kwong, Soke Chee |
| author_facet |
Kwong, Soke Chee |
| author_sort |
Kwong, Soke Chee |
| title |
Spinal motor neuronal organisation of selected local freshwater fishes in relation to median fin innervations / Kwong Soke Chee |
| title_short |
Spinal motor neuronal organisation of selected local freshwater fishes in relation to median fin innervations / Kwong Soke Chee |
| title_full |
Spinal motor neuronal organisation of selected local freshwater fishes in relation to median fin innervations / Kwong Soke Chee |
| title_fullStr |
Spinal motor neuronal organisation of selected local freshwater fishes in relation to median fin innervations / Kwong Soke Chee |
| title_full_unstemmed |
Spinal motor neuronal organisation of selected local freshwater fishes in relation to median fin innervations / Kwong Soke Chee |
| title_sort |
spinal motor neuronal organisation of selected local freshwater fishes in relation to median fin innervations / kwong soke chee |
| publishDate |
2012 |
| url |
http://studentsrepo.um.edu.my/3781/1/1._Title_page%2C_abstract%2C_content.pdf http://studentsrepo.um.edu.my/3781/2/2._Chapter_1_%E2%80%93_7.pdf http://studentsrepo.um.edu.my/3781/3/3._References.pdf http://studentsrepo.um.edu.my/3781/4/4._Appendices.pdf http://pendeta.um.edu.my/client/default/search/results?qu=Spinal+motor+neuronal+organisation+of+selected+local+freshwater+fishes+in+relation+to+median+fin+innervations&te= http://studentsrepo.um.edu.my/3781/ |
| _version_ |
1738505605892538368 |
| spelling |
my.um.stud.37812013-08-29T05:39:20Z Spinal motor neuronal organisation of selected local freshwater fishes in relation to median fin innervations / Kwong Soke Chee Kwong, Soke Chee QH301 Biology Fish fins are appendages which represent the limbs in higher vertebrates. In general, fish moves in the water by body undulation with the assistance of fins. To date, most of the studies focused on the innervations of limbs especially in mammals, such as rats, cats and monkeys. On the contrary, understanding on the innervations of the fish fin is still at its infancy as studies on the relevant field are relatively scarce. Hence, this study aimed to fill the knowledge gap, i.e. to investigate the organisation of spinal motor neurons in relation to the distribution of the median fins. For comparison purposes, four species of juvenile fishes utilised in this study were divided into two groups, namely (i) fish with long and continuous dorsal fin (Channa micropeltes, toman and Clarias sp., keli), and (ii) fish with short and non-continuous dorsal fin (Mystus nemurus, baung and Pangasius sp., patin). Spinal cord tissue obtained from three different representative segments along the rostro-caudal axis were processed histologically and stained using three neurohistological staining techniques including H&E, Nissl as well as Lillie’s Variant of the Weil-Weigert prior to light microscopy level observation. The organisation of motor neurons was correlated with the distribution of fins. Cell column organisation characterised the location of motor neurons into seven cell columns, namely intermediolateral (IL), dorsomedial (DM), ventromedial (VM), central (C), ventrolateral (VL), dorsoventral (DV) and retrodorsolateral (RDL) cell columns. It was observed that median motor neurons (IL, DM and VM cell columns) outnumbered central (C cell column) and lateral motor neurons (VL, DL and RDL cell columns) at all rostral, median, and caudal region of the fish body trunk. Medial motor neurons (IL and DM cell columns) were found in all three segments; thus, were probably innervating the body trunk, for instance to aid in body undulation. On the contrary, DL motor neurons were found only at the dorsal fin area of Clarias sp. and Pangasius sp.; thus, were probably innervating the particular fin. To further investigate the innervations of caudal fin muscles through HRP histochemistry, Clarias sp. with rounded caudal fin and Pangasius sp. with forked caudal fin were selected from each group. The nerve trunks at the caudal fin were transected and soaked in 50% HRP solution. After two days, each fish was sacrificed and its spinal cord was freeze-sectioned using cryostat. The sections were stained using DAB Liquid Enhanced Substrate kit to reveal the motor neurons containing HRP in the cytoplasm which appeared as brownish precipitations. Most of the motor neurons innervating Clarias sp. rounded caudal fin were located medially at IL, DM and VM cell columns. In contrast, the motor neurons innervating forked caudal fin of Pangasius sp. were located mostly at the ventral areas, namely VM and VL cell columns. The differences of the motor neuronal organisation between the two species could be credited to further muscle differentiation in the caudal fin muscle layers of Pangasius sp., which exhibited more complicated organisation than that of the Clarias sp. In conclusion, the findings of this study were in agreement with the existing literature that medial motor neurons (IL and DM cell columns) innervate body trunk while more laterally located motor neurons (VM, VL and DL cell columns) innervate distal structure, in this case the fins. Future research can focus, specifically, on the innervations by individual nerve trunk or innervations of individual muscle and/or the research can be conducted at different stages of life cycle of the fish. 2012 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/3781/1/1._Title_page%2C_abstract%2C_content.pdf application/pdf http://studentsrepo.um.edu.my/3781/2/2._Chapter_1_%E2%80%93_7.pdf application/pdf http://studentsrepo.um.edu.my/3781/3/3._References.pdf application/pdf http://studentsrepo.um.edu.my/3781/4/4._Appendices.pdf http://pendeta.um.edu.my/client/default/search/results?qu=Spinal+motor+neuronal+organisation+of+selected+local+freshwater+fishes+in+relation+to+median+fin+innervations&te= Kwong, Soke Chee (2012) Spinal motor neuronal organisation of selected local freshwater fishes in relation to median fin innervations / Kwong Soke Chee. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/3781/ |
| score |
13.211869 |