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Base population structure studies for Macrobrachium Rosenbergii using microsatellite markers / Thamayanthi Nada Raja

Giant freshwater prawn farming is startlingly increasing in Malaysia, yet little is known about the patterns of genetic diversity of its domesticated and wild stocks. Making use of populations with different gene resources is a way to increase genetic diversity of Macrobrachium rosenbergii in Malays...

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Main Author: Nada Raja, Thamayanthi
Format: Thesis
Published: 2011
Subjects:
Q Science (General)
QH301 Biology
Online Access:http://studentsrepo.um.edu.my/3526/4/Title_page%2C_abstract%2C_table_of_contents.pdf
http://studentsrepo.um.edu.my/3526/5/Full_chapters.pdf
http://studentsrepo.um.edu.my/3526/6/References.pdf
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spelling my.um.stud.35262013-09-26T04:01:33Z Base population structure studies for Macrobrachium Rosenbergii using microsatellite markers / Thamayanthi Nada Raja Nada Raja, Thamayanthi Q Science (General) QH301 Biology Giant freshwater prawn farming is startlingly increasing in Malaysia, yet little is known about the patterns of genetic diversity of its domesticated and wild stocks. Making use of populations with different gene resources is a way to increase genetic diversity of Macrobrachium rosenbergii in Malaysia. Availability of microsatellite markers (‘western’ form) which are highly polymorphic enables the divergence study of this species to be conducted for genetic improvement. This project successfully identified potential base populations for a genetic improvement program. Mean observed heterozygosity across four populations was in the range of 0.55 to 0.62 while the mean expected heterozygosity ranged from 0.85 to 0.87. Mean number of alleles was found to be the highest in the Kg Acheh population at 15.40. The highest genetic distance was found between Kampung Acheh and Sg Perak populations with a value of 1.29 with an Pairwise FST value of 0.15. Population Teluk Kumbar and Sg Perak showed a high genetic identity with a value of 0.57 and a low Pairwise FST value of 0.09. The Kg Acheh and Sg Perak populations were the most divergent among the other populations and are the best pair to be crossed. For a successful genetic improvement program, genetic relatedness between parents from different locations is crucial. Many farm operators are mixing different populations for the sake of genetic diversity without having a proper genetics data. The number of different populations does not confirm the accumulation of variant alleles where genetic bottleneck could happen without proper relatedness data. 2011 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/3526/4/Title_page%2C_abstract%2C_table_of_contents.pdf application/pdf http://studentsrepo.um.edu.my/3526/5/Full_chapters.pdf application/pdf http://studentsrepo.um.edu.my/3526/6/References.pdf http://pendeta.um.edu.my/client/default/search/results?qu=Base+population+structure+studies+for+Macrobrachium+Rosenbergii+using+microsatellite+markers&te= Nada Raja, Thamayanthi (2011) Base population structure studies for Macrobrachium Rosenbergii using microsatellite markers / Thamayanthi Nada Raja. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/3526/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic Q Science (General)
QH301 Biology
spellingShingle Q Science (General)
QH301 Biology
Nada Raja, Thamayanthi
Base population structure studies for Macrobrachium Rosenbergii using microsatellite markers / Thamayanthi Nada Raja
description Giant freshwater prawn farming is startlingly increasing in Malaysia, yet little is known about the patterns of genetic diversity of its domesticated and wild stocks. Making use of populations with different gene resources is a way to increase genetic diversity of Macrobrachium rosenbergii in Malaysia. Availability of microsatellite markers (‘western’ form) which are highly polymorphic enables the divergence study of this species to be conducted for genetic improvement. This project successfully identified potential base populations for a genetic improvement program. Mean observed heterozygosity across four populations was in the range of 0.55 to 0.62 while the mean expected heterozygosity ranged from 0.85 to 0.87. Mean number of alleles was found to be the highest in the Kg Acheh population at 15.40. The highest genetic distance was found between Kampung Acheh and Sg Perak populations with a value of 1.29 with an Pairwise FST value of 0.15. Population Teluk Kumbar and Sg Perak showed a high genetic identity with a value of 0.57 and a low Pairwise FST value of 0.09. The Kg Acheh and Sg Perak populations were the most divergent among the other populations and are the best pair to be crossed. For a successful genetic improvement program, genetic relatedness between parents from different locations is crucial. Many farm operators are mixing different populations for the sake of genetic diversity without having a proper genetics data. The number of different populations does not confirm the accumulation of variant alleles where genetic bottleneck could happen without proper relatedness data.
format Thesis
author Nada Raja, Thamayanthi
author_facet Nada Raja, Thamayanthi
author_sort Nada Raja, Thamayanthi
title Base population structure studies for Macrobrachium Rosenbergii using microsatellite markers / Thamayanthi Nada Raja
title_short Base population structure studies for Macrobrachium Rosenbergii using microsatellite markers / Thamayanthi Nada Raja
title_full Base population structure studies for Macrobrachium Rosenbergii using microsatellite markers / Thamayanthi Nada Raja
title_fullStr Base population structure studies for Macrobrachium Rosenbergii using microsatellite markers / Thamayanthi Nada Raja
title_full_unstemmed Base population structure studies for Macrobrachium Rosenbergii using microsatellite markers / Thamayanthi Nada Raja
title_sort base population structure studies for macrobrachium rosenbergii using microsatellite markers / thamayanthi nada raja
publishDate 2011
url http://studentsrepo.um.edu.my/3526/4/Title_page%2C_abstract%2C_table_of_contents.pdf
http://studentsrepo.um.edu.my/3526/5/Full_chapters.pdf
http://studentsrepo.um.edu.my/3526/6/References.pdf
http://pendeta.um.edu.my/client/default/search/results?qu=Base+population+structure+studies+for+Macrobrachium+Rosenbergii+using+microsatellite+markers&te=
http://studentsrepo.um.edu.my/3526/
_version_ 1738505578758537216
score 13.211869

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