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Characterization of microrna172 and its potential target in oil palm (Elaeis guineensis Jaq.)

Oil palm (Elaeis guineensis) is the world’s major perennial monocot crop and contributes highly to the economies of South East Asia. As the oil palm fruit is the source of the main economic output for this crop, floral development is a critical process related to oil palm productivity. miR172 has be...

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Main Author:	Fatemeh, M.
Format:	Thesis
Published:	2010
Subjects:	Q Science (General) R Medicine (General) S Agriculture (General)
Online Access:	http://studentsrepo.um.edu.my/3498/1/COVER.pdf http://studentsrepo.um.edu.my/3498/2/Page_1.pdf http://studentsrepo.um.edu.my/3498/3/(0)_ABSTRACT.pdf http://studentsrepo.um.edu.my/3498/4/List_of_Contents_(new).pdf http://studentsrepo.um.edu.my/3498/5/Ch_1%2C2%2C3%2C4%2C5.pdf http://studentsrepo.um.edu.my/3498/7/REFERENCES.pdf http://studentsrepo.um.edu.my/3498/6/Appendix.pdf http://studentsrepo.um.edu.my/3498/
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spelling	my.um.stud.34982013-07-10T04:21:17Z Characterization of microrna172 and its potential target in oil palm (Elaeis guineensis Jaq.) Fatemeh, M. Q Science (General) R Medicine (General) S Agriculture (General) Oil palm (Elaeis guineensis) is the world’s major perennial monocot crop and contributes highly to the economies of South East Asia. As the oil palm fruit is the source of the main economic output for this crop, floral development is a critical process related to oil palm productivity. miR172 has been shown to be an important regulator of the AP2 family transcription factors involved in floral timing in a number of plants and belongs to a group of highly conserved miRNA that can be identified based on sequence homology. Six oil palm miR172 paralogs, representing two distinct mature miR172 sequences, and an AP2-like target were identified based on sequence identity and predicted hairpin structures, using a combination of RT-PCR cloning and bioinformatic analysis of oil palm EST data. Expression of mature miR172a sequences was higher in floral organs than vegetative tissues, confirming the role of this miRNA in floral organ identity and flowering time. Some variation in the expression of the six paralogs egu-miR172 precursors in different oil palm tissues was observed with each of the two isoforms showing different expression patterns during floral development. miR172 and the AP2-like gene showed an inverse expression pattern to that of the predicted AP2-like target in some tissues while they also showed overlapping expression patterns and did not show an obvious negative correlation in some other tissues. These data are consistent with previous reports that although in some tissues the cleavage of the potential target was observed, miR172 may also act via translational repression. 2010 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/3498/1/COVER.pdf application/pdf http://studentsrepo.um.edu.my/3498/2/Page_1.pdf application/pdf http://studentsrepo.um.edu.my/3498/3/(0)_ABSTRACT.pdf application/pdf http://studentsrepo.um.edu.my/3498/4/List_of_Contents_(new).pdf application/pdf http://studentsrepo.um.edu.my/3498/5/Ch_1%2C2%2C3%2C4%2C5.pdf application/pdf http://studentsrepo.um.edu.my/3498/7/REFERENCES.pdf application/pdf http://studentsrepo.um.edu.my/3498/6/Appendix.pdf Fatemeh, M. (2010) Characterization of microrna172 and its potential target in oil palm (Elaeis guineensis Jaq.). Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/3498/
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Student Repository
url_provider	http://studentsrepo.um.edu.my/
topic	Q Science (General) R Medicine (General) S Agriculture (General)
spellingShingle	Q Science (General) R Medicine (General) S Agriculture (General) Fatemeh, M. Characterization of microrna172 and its potential target in oil palm (Elaeis guineensis Jaq.)
description	Oil palm (Elaeis guineensis) is the world’s major perennial monocot crop and contributes highly to the economies of South East Asia. As the oil palm fruit is the source of the main economic output for this crop, floral development is a critical process related to oil palm productivity. miR172 has been shown to be an important regulator of the AP2 family transcription factors involved in floral timing in a number of plants and belongs to a group of highly conserved miRNA that can be identified based on sequence homology. Six oil palm miR172 paralogs, representing two distinct mature miR172 sequences, and an AP2-like target were identified based on sequence identity and predicted hairpin structures, using a combination of RT-PCR cloning and bioinformatic analysis of oil palm EST data. Expression of mature miR172a sequences was higher in floral organs than vegetative tissues, confirming the role of this miRNA in floral organ identity and flowering time. Some variation in the expression of the six paralogs egu-miR172 precursors in different oil palm tissues was observed with each of the two isoforms showing different expression patterns during floral development. miR172 and the AP2-like gene showed an inverse expression pattern to that of the predicted AP2-like target in some tissues while they also showed overlapping expression patterns and did not show an obvious negative correlation in some other tissues. These data are consistent with previous reports that although in some tissues the cleavage of the potential target was observed, miR172 may also act via translational repression.
format	Thesis
author	Fatemeh, M.
author_facet	Fatemeh, M.
author_sort	Fatemeh, M.
title	Characterization of microrna172 and its potential target in oil palm (Elaeis guineensis Jaq.)
title_short	Characterization of microrna172 and its potential target in oil palm (Elaeis guineensis Jaq.)
title_full	Characterization of microrna172 and its potential target in oil palm (Elaeis guineensis Jaq.)
title_fullStr	Characterization of microrna172 and its potential target in oil palm (Elaeis guineensis Jaq.)
title_full_unstemmed	Characterization of microrna172 and its potential target in oil palm (Elaeis guineensis Jaq.)
title_sort	characterization of microrna172 and its potential target in oil palm (elaeis guineensis jaq.)
publishDate	2010
url	http://studentsrepo.um.edu.my/3498/1/COVER.pdf http://studentsrepo.um.edu.my/3498/2/Page_1.pdf http://studentsrepo.um.edu.my/3498/3/(0)_ABSTRACT.pdf http://studentsrepo.um.edu.my/3498/4/List_of_Contents_(new).pdf http://studentsrepo.um.edu.my/3498/5/Ch_1%2C2%2C3%2C4%2C5.pdf http://studentsrepo.um.edu.my/3498/7/REFERENCES.pdf http://studentsrepo.um.edu.my/3498/6/Appendix.pdf http://studentsrepo.um.edu.my/3498/
_version_	1738505575381073920
score	13.211869

Characterization of microrna172 and its potential target in oil palm (Elaeis guineensis Jaq.)

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