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Anti-inflammatory effect of single and combined Christia vespertilionis leaf extract and palm tocotrienol-rich fraction / Izzah Farhah Zambari

Christia vespertilionis were used traditionally to treat inflammation. Non-Steroidal Drugs (NSAIDs) are commonly prescribed to treat inflammation. However, long-term prescription of these NSAIDs will lead to adverse side-effect. This study was performed to evaluate anti-inflammatory properties of C....

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Main Author: Izzah Farhah , Zambari
Format: Thesis
Published: 2024
Subjects:
Q Science (General)
QH301 Biology
Online Access:http://studentsrepo.um.edu.my/15506/2/Izzah_Farhah.pdf
http://studentsrepo.um.edu.my/15506/1/Izzah_Farhah.pdf
http://studentsrepo.um.edu.my/15506/
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spelling my.um.stud.155062025-01-08T21:54:23Z Anti-inflammatory effect of single and combined Christia vespertilionis leaf extract and palm tocotrienol-rich fraction / Izzah Farhah Zambari Izzah Farhah , Zambari Q Science (General) QH301 Biology Christia vespertilionis were used traditionally to treat inflammation. Non-Steroidal Drugs (NSAIDs) are commonly prescribed to treat inflammation. However, long-term prescription of these NSAIDs will lead to adverse side-effect. This study was performed to evaluate anti-inflammatory properties of C. vespertilionis leaves besides purifying its protease and to determining its anti-inflammatory effect through in vitro and in vivo studies of extract combination with palm tocotrienol-rich fraction (TRF). Result showed that C. vespertilionis extract analysed using GC-MS contain anti-inflammatory compounds. Enzymatic protease was successfully extracted and purified through ammonium sulphate precipitation with further dialysis increases its yield by 53 % with specific activity 14.33 U/mg. The combination between C. vespertilionis protease (CVP) and palm tocotrienol-rich fraction (TRF) were tested synergistically to reduce inflammation were done through in vitro and in vivo studies and it shows that the combination of CVP and TRF (CVP+TRF) at the ratio of 14.10:8.48 μg/mL, gives 50 % inhibition (IC50) of nitric oxide (NO). In conclusion, this study managed to show the synergistic effect of the combination of CVP and TRF against LPS-stimulated RAW264.7 cells and carrageenan-induced BALB/c paw oedema. The treatment could be potentially effective in reducing inflammation, and thus could be an alternative approach to NSAIDs drugs. 2024-07 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/15506/2/Izzah_Farhah.pdf application/pdf http://studentsrepo.um.edu.my/15506/1/Izzah_Farhah.pdf Izzah Farhah , Zambari (2024) Anti-inflammatory effect of single and combined Christia vespertilionis leaf extract and palm tocotrienol-rich fraction / Izzah Farhah Zambari. PhD thesis, Universiti Malaya. http://studentsrepo.um.edu.my/15506/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic Q Science (General)
QH301 Biology
spellingShingle Q Science (General)
QH301 Biology
Izzah Farhah , Zambari
Anti-inflammatory effect of single and combined Christia vespertilionis leaf extract and palm tocotrienol-rich fraction / Izzah Farhah Zambari
description Christia vespertilionis were used traditionally to treat inflammation. Non-Steroidal Drugs (NSAIDs) are commonly prescribed to treat inflammation. However, long-term prescription of these NSAIDs will lead to adverse side-effect. This study was performed to evaluate anti-inflammatory properties of C. vespertilionis leaves besides purifying its protease and to determining its anti-inflammatory effect through in vitro and in vivo studies of extract combination with palm tocotrienol-rich fraction (TRF). Result showed that C. vespertilionis extract analysed using GC-MS contain anti-inflammatory compounds. Enzymatic protease was successfully extracted and purified through ammonium sulphate precipitation with further dialysis increases its yield by 53 % with specific activity 14.33 U/mg. The combination between C. vespertilionis protease (CVP) and palm tocotrienol-rich fraction (TRF) were tested synergistically to reduce inflammation were done through in vitro and in vivo studies and it shows that the combination of CVP and TRF (CVP+TRF) at the ratio of 14.10:8.48 μg/mL, gives 50 % inhibition (IC50) of nitric oxide (NO). In conclusion, this study managed to show the synergistic effect of the combination of CVP and TRF against LPS-stimulated RAW264.7 cells and carrageenan-induced BALB/c paw oedema. The treatment could be potentially effective in reducing inflammation, and thus could be an alternative approach to NSAIDs drugs.
format Thesis
author Izzah Farhah , Zambari
author_facet Izzah Farhah , Zambari
author_sort Izzah Farhah , Zambari
title Anti-inflammatory effect of single and combined Christia vespertilionis leaf extract and palm tocotrienol-rich fraction / Izzah Farhah Zambari
title_short Anti-inflammatory effect of single and combined Christia vespertilionis leaf extract and palm tocotrienol-rich fraction / Izzah Farhah Zambari
title_full Anti-inflammatory effect of single and combined Christia vespertilionis leaf extract and palm tocotrienol-rich fraction / Izzah Farhah Zambari
title_fullStr Anti-inflammatory effect of single and combined Christia vespertilionis leaf extract and palm tocotrienol-rich fraction / Izzah Farhah Zambari
title_full_unstemmed Anti-inflammatory effect of single and combined Christia vespertilionis leaf extract and palm tocotrienol-rich fraction / Izzah Farhah Zambari
title_sort anti-inflammatory effect of single and combined christia vespertilionis leaf extract and palm tocotrienol-rich fraction / izzah farhah zambari
publishDate 2024
url http://studentsrepo.um.edu.my/15506/2/Izzah_Farhah.pdf
http://studentsrepo.um.edu.my/15506/1/Izzah_Farhah.pdf
http://studentsrepo.um.edu.my/15506/
_version_ 1823273222005587968
score 13.244413

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