Label-free quantitative analysis of endometrial receptivity proteins in infertile women with noncavity-distorting fibroids / An-Nisaa Jasmine Mohd Roslee
Uterine fibroids (leiomyomas) pose significant challenges to women of reproductive age, impacting fertility, menstrual health, and overall well-being. This study investigates the intricate relationship between noncavity-distorting intramural fibroids and endometrial receptivity, shedding light on mo...
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| Format: | Thesis |
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2024
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| Online Access: | http://studentsrepo.um.edu.my/15456/2/An%2DNisaa_Jasmine.pdf http://studentsrepo.um.edu.my/15456/1/An%2DNisaa_Jasmine.pdf http://studentsrepo.um.edu.my/15456/ |
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| Summary: | Uterine fibroids (leiomyomas) pose significant challenges to women of reproductive age, impacting fertility, menstrual health, and overall well-being. This study investigates the intricate relationship between noncavity-distorting intramural fibroids and endometrial receptivity, shedding light on molecular disruptions that affect fertility treatment outcomes, particularly in assisted reproductive technologies like in vitro fertilisation (IVF) or intracytoplasmic sperm injection (ICSI). The prevalence of uterine fibroids and their association with heavy menstrual bleeding, anaemia, and infertility emphasises the need for a comprehensive understanding of their impact on reproductive health. The response of fibroids to reproductive hormones, particularly oestrogen and progesterone, coupled with their potential to distort the uterine cavity, further complicates the implantation process during fertility treatments. This research employs label-free quantitative mass spectrometry to profile the endometrial receptivity proteome in three distinct groups: infertile women with intramural fibroids, infertile women without fibroids, and fertile women. The comprehensive identification of 2,089 proteins reveals significant alterations in the protein landscape associated with intramural fibroids. Notable proteins, including moesin, nucleophosmin, annexin A2, annexin A7, complement component 3, calreticulin, protein disulphide isomerase 3, and serotransferrin, demonstrate differential expressions in infertile women with fibroids. These proteins play crucial roles in cellular processes such as actin cytoskeleton regulation, cell adhesion, and calcium signalling, which are essential for endometrial receptivity. The study delves into the impact of these proteins on cellular functions, exploring alterations in extracellular exosomes, unfolded protein binding, and immune responses. Additionally, the observed endoplasmic reticulum (ER) stress suggests a multifaceted role of intramural fibroids in influencing the endometrial microenvironment and receptivity. The study's findings highlight the complex interplay between extracellular matrix (ECM) production, inflammation, and ER stress, offering valuable insights into potential disruptions in endometrial receptivity associated with noncavity-distorting fibroids. This understanding is critical for tailoring fertility treatments to address the specific challenges posed by fibroids in infertile women. In conclusion, this research contributes to the growing body of knowledge on the impact of uterine fibroids on reproductive health. Identifying specific proteins associated with intramural fibroids provides a foundation for future studies and potential therapeutic interventions. By unravelling the molecular disruptions affecting endometrial receptivity, this study offers a pathway toward improved fertility treatment strategies for women with fibroids.
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