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Antimicrobial activity of silver nanoparticles for the control of microbial spoilage of grey oyster mushroom (Pleurotus pulmonarius) / Sugendra Kumar Vinodamaney

Grey oyster mushroom (Pleurotus pulmonarius) is one of the most popular mushrooms in Malaysia and contains a high amount of nutrients. However, the fresh grey oyster mushroom is having short shelf life thus reducing its quality and economic value. Limited study on maintaining the fresh quality and p...

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Main Author: Sugendra Kumar , Vinodamaney
Format: Thesis
Published: 2022
Subjects:
Q Science (General)
QH301 Biology
Online Access:http://studentsrepo.um.edu.my/15324/1/Sugendra_Kumar.pdf
http://studentsrepo.um.edu.my/15324/2/Sugendra.pdf
http://studentsrepo.um.edu.my/15324/
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spelling my.um.stud.153242024-11-05T22:58:28Z Antimicrobial activity of silver nanoparticles for the control of microbial spoilage of grey oyster mushroom (Pleurotus pulmonarius) / Sugendra Kumar Vinodamaney Sugendra Kumar , Vinodamaney Q Science (General) QH301 Biology Grey oyster mushroom (Pleurotus pulmonarius) is one of the most popular mushrooms in Malaysia and contains a high amount of nutrients. However, the fresh grey oyster mushroom is having short shelf life thus reducing its quality and economic value. Limited study on maintaining the fresh quality and prolonging the shelf life of the fresh grey oyster mushroom. Silver nanoparticles (Ag-NPs) are known to act as antimicrobial agents against a wider range of microorganisms. Therefore, this study aimed to identify the 11 spoilage bacteria strains isolated from rotten grey oyster mushrooms and evaluate the antimicrobial activity of different sizes of Ag-NPs (30.88 nm, 60.52 nm, 99.16 nm) to inhibit the growth of spoilage bacteria using the broth microdilution method. The spoilage bacterial strains were identified as Raoultella ornithinolytica, Raoultella planticola, Klebsiella sp., Enterobacter cloacae, and Cedecea neteri based on biochemical assay and 16S rRNA sequencing. At 50 μg/ml, Ag-NPs (30.88 nm) showed inhibition (ranged from 99.21-100%) on the growth of the 11 strains of spoilage bacteria. Also, Ag-NPs 30.88 nm and Ag-NPs 60.52 nm exhibited higher minimum inhibitory concentration to the spoilage bacteria as compared to Ag-NPs 99.16 nm. In conclusion, smaller-sized Ag-NPs have higher bacterial inhibitory capacity compared to large-sized Ag-NPs. 2022-04 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/15324/1/Sugendra_Kumar.pdf application/pdf http://studentsrepo.um.edu.my/15324/2/Sugendra.pdf Sugendra Kumar , Vinodamaney (2022) Antimicrobial activity of silver nanoparticles for the control of microbial spoilage of grey oyster mushroom (Pleurotus pulmonarius) / Sugendra Kumar Vinodamaney. Masters thesis, Universiti Malaya. http://studentsrepo.um.edu.my/15324/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic Q Science (General)
QH301 Biology
spellingShingle Q Science (General)
QH301 Biology
Sugendra Kumar , Vinodamaney
Antimicrobial activity of silver nanoparticles for the control of microbial spoilage of grey oyster mushroom (Pleurotus pulmonarius) / Sugendra Kumar Vinodamaney
description Grey oyster mushroom (Pleurotus pulmonarius) is one of the most popular mushrooms in Malaysia and contains a high amount of nutrients. However, the fresh grey oyster mushroom is having short shelf life thus reducing its quality and economic value. Limited study on maintaining the fresh quality and prolonging the shelf life of the fresh grey oyster mushroom. Silver nanoparticles (Ag-NPs) are known to act as antimicrobial agents against a wider range of microorganisms. Therefore, this study aimed to identify the 11 spoilage bacteria strains isolated from rotten grey oyster mushrooms and evaluate the antimicrobial activity of different sizes of Ag-NPs (30.88 nm, 60.52 nm, 99.16 nm) to inhibit the growth of spoilage bacteria using the broth microdilution method. The spoilage bacterial strains were identified as Raoultella ornithinolytica, Raoultella planticola, Klebsiella sp., Enterobacter cloacae, and Cedecea neteri based on biochemical assay and 16S rRNA sequencing. At 50 μg/ml, Ag-NPs (30.88 nm) showed inhibition (ranged from 99.21-100%) on the growth of the 11 strains of spoilage bacteria. Also, Ag-NPs 30.88 nm and Ag-NPs 60.52 nm exhibited higher minimum inhibitory concentration to the spoilage bacteria as compared to Ag-NPs 99.16 nm. In conclusion, smaller-sized Ag-NPs have higher bacterial inhibitory capacity compared to large-sized Ag-NPs.
format Thesis
author Sugendra Kumar , Vinodamaney
author_facet Sugendra Kumar , Vinodamaney
author_sort Sugendra Kumar , Vinodamaney
title Antimicrobial activity of silver nanoparticles for the control of microbial spoilage of grey oyster mushroom (Pleurotus pulmonarius) / Sugendra Kumar Vinodamaney
title_short Antimicrobial activity of silver nanoparticles for the control of microbial spoilage of grey oyster mushroom (Pleurotus pulmonarius) / Sugendra Kumar Vinodamaney
title_full Antimicrobial activity of silver nanoparticles for the control of microbial spoilage of grey oyster mushroom (Pleurotus pulmonarius) / Sugendra Kumar Vinodamaney
title_fullStr Antimicrobial activity of silver nanoparticles for the control of microbial spoilage of grey oyster mushroom (Pleurotus pulmonarius) / Sugendra Kumar Vinodamaney
title_full_unstemmed Antimicrobial activity of silver nanoparticles for the control of microbial spoilage of grey oyster mushroom (Pleurotus pulmonarius) / Sugendra Kumar Vinodamaney
title_sort antimicrobial activity of silver nanoparticles for the control of microbial spoilage of grey oyster mushroom (pleurotus pulmonarius) / sugendra kumar vinodamaney
publishDate 2022
url http://studentsrepo.um.edu.my/15324/1/Sugendra_Kumar.pdf
http://studentsrepo.um.edu.my/15324/2/Sugendra.pdf
http://studentsrepo.um.edu.my/15324/
_version_ 1816130799239430144
score 13.226497

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