Cover Image

DNA-specific schottky diodes for detecting viruses infection in penaeid shrimp / Chan Yen Yew

DNA-based technology has a broad perspective in different applications such as cryptography, information storage, basic logic gates, nanorobots, nanocircuits and nanomedicine as well as in diagnostic. In aquaculture, especially shrimp farming is facing great challenges in preventing bacterial and vi...

Full description

Saved in:
Bibliographic Details
Main Author: Chan , Yen Yew
Format: Thesis
Published: 2023
Subjects:
Q Science (General)
QC Physics
Online Access:http://studentsrepo.um.edu.my/15027/1/Chan_Yen_Yew.pdf
http://studentsrepo.um.edu.my/15027/2/Chan_Yen_Yew.pdf
http://studentsrepo.um.edu.my/15027/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.stud.15027
record_format eprints
spelling my.um.stud.150272025-01-20T17:35:35Z DNA-specific schottky diodes for detecting viruses infection in penaeid shrimp / Chan Yen Yew Chan , Yen Yew Q Science (General) QC Physics DNA-based technology has a broad perspective in different applications such as cryptography, information storage, basic logic gates, nanorobots, nanocircuits and nanomedicine as well as in diagnostic. In aquaculture, especially shrimp farming is facing great challenges in preventing bacterial and viral caused diseases which devastates this blooming food industry. However, such problems could be significantly reduced using effective diagnostic methods. DNA diagnostic techniques involves conventional molecular biology diagnostic technique (PCR, LAMP, qPCR, ELISA etc), while new approaches in DNA biosensor (piezoelectric, optical transducer, thermometric and electrochemical) are still being developing. In this work, potential electronic DNA biosensors for electronically characterizing and detecting selected disease-causing viruses is proposed. DNA Schottky diodes are implemented to identify DNA viruses (MBV, IHHNV, WSSV) and RNA viruses (TSV, YHV) in shrimp industry that poses heavy threat to the aquaculture community. With modification of the architecture of the Schottky diode, Al/ITO/DNA/Al and Cu/DNA/Cu are presented to evaluate the effect of DNA and RNA characterization of the viruses. Acquisition and analysis of the current-voltage (I-V) characterization provides the insight of solid-state parameters of the viruses corresponding to the sequence length and polynucleotide arrangement. In a comparison study, selected bacterial DNA employed in the viral characterization study were observed that viruses and bacteria I-V profile showed distinct band structures using Al/ITO/DNA/Al junction. In the solid-state calculation with conventional diode and Cheung’s models, that bacteria profiles possessed higher series resistance and ideality factor while had lower threshold voltage than viral profiles using Al/ITO/DNA/Al junction. These results suggest that the sequence size and polynucleotide arrangement of the DNA sequence affect rectification and charge transport system of DNA biopolymer. Each DNA-specific Schottky diode generated characteristic I-V profiles similar to a semiconducting behavior. DNA-based Schottky diode biosensor showed the rapid biosensing potential to differentiate pathogens from shrimps as the current diagnostic method involves expensive cost and tedious procedures. 2023-01 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/15027/1/Chan_Yen_Yew.pdf application/pdf http://studentsrepo.um.edu.my/15027/2/Chan_Yen_Yew.pdf Chan , Yen Yew (2023) DNA-specific schottky diodes for detecting viruses infection in penaeid shrimp / Chan Yen Yew. PhD thesis, Universiti Malaya. http://studentsrepo.um.edu.my/15027/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic Q Science (General)
QC Physics
spellingShingle Q Science (General)
QC Physics
Chan , Yen Yew
DNA-specific schottky diodes for detecting viruses infection in penaeid shrimp / Chan Yen Yew
description DNA-based technology has a broad perspective in different applications such as cryptography, information storage, basic logic gates, nanorobots, nanocircuits and nanomedicine as well as in diagnostic. In aquaculture, especially shrimp farming is facing great challenges in preventing bacterial and viral caused diseases which devastates this blooming food industry. However, such problems could be significantly reduced using effective diagnostic methods. DNA diagnostic techniques involves conventional molecular biology diagnostic technique (PCR, LAMP, qPCR, ELISA etc), while new approaches in DNA biosensor (piezoelectric, optical transducer, thermometric and electrochemical) are still being developing. In this work, potential electronic DNA biosensors for electronically characterizing and detecting selected disease-causing viruses is proposed. DNA Schottky diodes are implemented to identify DNA viruses (MBV, IHHNV, WSSV) and RNA viruses (TSV, YHV) in shrimp industry that poses heavy threat to the aquaculture community. With modification of the architecture of the Schottky diode, Al/ITO/DNA/Al and Cu/DNA/Cu are presented to evaluate the effect of DNA and RNA characterization of the viruses. Acquisition and analysis of the current-voltage (I-V) characterization provides the insight of solid-state parameters of the viruses corresponding to the sequence length and polynucleotide arrangement. In a comparison study, selected bacterial DNA employed in the viral characterization study were observed that viruses and bacteria I-V profile showed distinct band structures using Al/ITO/DNA/Al junction. In the solid-state calculation with conventional diode and Cheung’s models, that bacteria profiles possessed higher series resistance and ideality factor while had lower threshold voltage than viral profiles using Al/ITO/DNA/Al junction. These results suggest that the sequence size and polynucleotide arrangement of the DNA sequence affect rectification and charge transport system of DNA biopolymer. Each DNA-specific Schottky diode generated characteristic I-V profiles similar to a semiconducting behavior. DNA-based Schottky diode biosensor showed the rapid biosensing potential to differentiate pathogens from shrimps as the current diagnostic method involves expensive cost and tedious procedures.
format Thesis
author Chan , Yen Yew
author_facet Chan , Yen Yew
author_sort Chan , Yen Yew
title DNA-specific schottky diodes for detecting viruses infection in penaeid shrimp / Chan Yen Yew
title_short DNA-specific schottky diodes for detecting viruses infection in penaeid shrimp / Chan Yen Yew
title_full DNA-specific schottky diodes for detecting viruses infection in penaeid shrimp / Chan Yen Yew
title_fullStr DNA-specific schottky diodes for detecting viruses infection in penaeid shrimp / Chan Yen Yew
title_full_unstemmed DNA-specific schottky diodes for detecting viruses infection in penaeid shrimp / Chan Yen Yew
title_sort dna-specific schottky diodes for detecting viruses infection in penaeid shrimp / chan yen yew
publishDate 2023
url http://studentsrepo.um.edu.my/15027/1/Chan_Yen_Yew.pdf
http://studentsrepo.um.edu.my/15027/2/Chan_Yen_Yew.pdf
http://studentsrepo.um.edu.my/15027/
_version_ 1823273195282628608
score 13.23648

Similar Items