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Gold nanosphere-thioctic acid-Zn(Salophen) conjugate: Synthesis, characterisation, toxicity and protein interaction / Ng Yin Zhuang

A novel inorganic metal complex gold nanoparticle conjugate was synthesised and characterised in this study. It was achieved by conjugating a Schiff base zinc complex, [N,N-bis(salicylidene)-1,2-phenylenediamine]zinc(II) or commonly known as Zn(salophen) (ZnS) to gold nanosphere with thioctic acid f...

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Main Author: Ng , Yin Zhuang
Format: Thesis
Published: 2022
Subjects:
Q Science (General)
QD Chemistry
Online Access:http://studentsrepo.um.edu.my/14736/2/Ng_Yin_Zhuang.pdf
http://studentsrepo.um.edu.my/14736/1/Ng_Yin_Zhuang.pdf
http://studentsrepo.um.edu.my/14736/
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spelling my.um.stud.147362024-01-21T23:53:01Z Gold nanosphere-thioctic acid-Zn(Salophen) conjugate: Synthesis, characterisation, toxicity and protein interaction / Ng Yin Zhuang Ng , Yin Zhuang Q Science (General) QD Chemistry A novel inorganic metal complex gold nanoparticle conjugate was synthesised and characterised in this study. It was achieved by conjugating a Schiff base zinc complex, [N,N-bis(salicylidene)-1,2-phenylenediamine]zinc(II) or commonly known as Zn(salophen) (ZnS) to gold nanosphere with thioctic acid functioning as the linker. The conjugate (AuNS-TA-ZnS) was characterised with FTIR, UV-visible spectroscopy, fluorescence spectroscopy, dynamic light scattering (DLS) analysis, zeta potential analysis, inductively coupled plasma mass spectrometry (ICP-MS) analysis and transmission electron microscope (TEM). Both AuNS-TA-ZnS and ZnS were confirmed to be the proposed structures. The diameter of AuNS-TA-ZnS was determined to be 17.73 nm with 1785 ZnS conjugated to each gold nanosphere. The interactions of AuNS-TA-ZnS with proteins were studied. It was found that the conjugate has strong affinity to Bovine Serum Albumin (BSA). AuNS-TA-ZnS is able to alter the secondary structure of BSA without causing conformational change to the tertiary structure of BSA. Apart from demonstrating the ability to generate reactive oxygen species (ROS), AuNS-TA-ZnS also inhibits proteasome activities specifically at the Trypsin-like site of mouse 20S proteasome. Cytotoxicity study with breast cancer cells shows that AuNS-Ta-ZnS is more cytotoxic than unconjugated ZnS. Transepithelial electrical resistance (TEER) measurement across Caco-2 cell monolayer found AuNS-TA-ZnS has higher efficiency than ZnS in permeating thru the cells. 2022-06 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/14736/2/Ng_Yin_Zhuang.pdf application/pdf http://studentsrepo.um.edu.my/14736/1/Ng_Yin_Zhuang.pdf Ng , Yin Zhuang (2022) Gold nanosphere-thioctic acid-Zn(Salophen) conjugate: Synthesis, characterisation, toxicity and protein interaction / Ng Yin Zhuang. Masters thesis, Universiti Malaya. http://studentsrepo.um.edu.my/14736/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic Q Science (General)
QD Chemistry
spellingShingle Q Science (General)
QD Chemistry
Ng , Yin Zhuang
Gold nanosphere-thioctic acid-Zn(Salophen) conjugate: Synthesis, characterisation, toxicity and protein interaction / Ng Yin Zhuang
description A novel inorganic metal complex gold nanoparticle conjugate was synthesised and characterised in this study. It was achieved by conjugating a Schiff base zinc complex, [N,N-bis(salicylidene)-1,2-phenylenediamine]zinc(II) or commonly known as Zn(salophen) (ZnS) to gold nanosphere with thioctic acid functioning as the linker. The conjugate (AuNS-TA-ZnS) was characterised with FTIR, UV-visible spectroscopy, fluorescence spectroscopy, dynamic light scattering (DLS) analysis, zeta potential analysis, inductively coupled plasma mass spectrometry (ICP-MS) analysis and transmission electron microscope (TEM). Both AuNS-TA-ZnS and ZnS were confirmed to be the proposed structures. The diameter of AuNS-TA-ZnS was determined to be 17.73 nm with 1785 ZnS conjugated to each gold nanosphere. The interactions of AuNS-TA-ZnS with proteins were studied. It was found that the conjugate has strong affinity to Bovine Serum Albumin (BSA). AuNS-TA-ZnS is able to alter the secondary structure of BSA without causing conformational change to the tertiary structure of BSA. Apart from demonstrating the ability to generate reactive oxygen species (ROS), AuNS-TA-ZnS also inhibits proteasome activities specifically at the Trypsin-like site of mouse 20S proteasome. Cytotoxicity study with breast cancer cells shows that AuNS-Ta-ZnS is more cytotoxic than unconjugated ZnS. Transepithelial electrical resistance (TEER) measurement across Caco-2 cell monolayer found AuNS-TA-ZnS has higher efficiency than ZnS in permeating thru the cells.
format Thesis
author Ng , Yin Zhuang
author_facet Ng , Yin Zhuang
author_sort Ng , Yin Zhuang
title Gold nanosphere-thioctic acid-Zn(Salophen) conjugate: Synthesis, characterisation, toxicity and protein interaction / Ng Yin Zhuang
title_short Gold nanosphere-thioctic acid-Zn(Salophen) conjugate: Synthesis, characterisation, toxicity and protein interaction / Ng Yin Zhuang
title_full Gold nanosphere-thioctic acid-Zn(Salophen) conjugate: Synthesis, characterisation, toxicity and protein interaction / Ng Yin Zhuang
title_fullStr Gold nanosphere-thioctic acid-Zn(Salophen) conjugate: Synthesis, characterisation, toxicity and protein interaction / Ng Yin Zhuang
title_full_unstemmed Gold nanosphere-thioctic acid-Zn(Salophen) conjugate: Synthesis, characterisation, toxicity and protein interaction / Ng Yin Zhuang
title_sort gold nanosphere-thioctic acid-zn(salophen) conjugate: synthesis, characterisation, toxicity and protein interaction / ng yin zhuang
publishDate 2022
url http://studentsrepo.um.edu.my/14736/2/Ng_Yin_Zhuang.pdf
http://studentsrepo.um.edu.my/14736/1/Ng_Yin_Zhuang.pdf
http://studentsrepo.um.edu.my/14736/
_version_ 1789424902551371776
score 13.211869

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