Investigation of inflammatory responses following the use of novel anisotropic hydrogel tissue expander in the skin of maxillofacial region / Kholoud Mohamed Ali Salim Igdayir
Background: The purpose of tissue expander is to grow healthy supplementary tissue under the controlled of mechanical force exerted by the tissue expander. In the field of reconstructive and plastic surgery, the use of soft tissue expanders are important for growing healthy supplementary tissue....
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Published: |
2020
|
| Subjects: | |
| Online Access: | http://studentsrepo.um.edu.my/11700/4/kholoud.pdf http://studentsrepo.um.edu.my/11700/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Background: The purpose of tissue expander is to grow healthy supplementary tissue
under the controlled of mechanical force exerted by the tissue expander. In the field of
reconstructive and plastic surgery, the use of soft tissue expanders are important for
growing healthy supplementary tissue. However, the molecular mechanism associated
with the inflammatory response associated with the force generated from the use of
tissue expanders is still not characterized. Among the many types of tissue expanders,
anisotropic type of hydrogel tissue expander was chosen due to its ability to produce
controlled incremental inflation on the tissue that enhance cellular adaptation and the
ability of the cells to resist mechanical injury. Objective: To determine whether
anisotropic hydrogel tissue expander promotes subclinical inflammatory response by
triggering the secretion of pro-inflammatory cytokines. Material and Methods: Seven, 8
weeks old Sprague Dawley rats (300g ± 50g) were used as models. The rats were
randomly divided into two groups: control group (n=3) and expanded group (n=4). The
expanders were implanted subcutaneously at the forehead region for 28 days and
surgically removed. The rats were sacrificed and skin samples were harvested and
stored in 10% formaldehyde, fixed in formalin, and embedded in paraffin wax for
histological investigation. Hematoxylin and eosin staining was performed to detect
histological changes between the two groups and to detect the presence of an
inflammatory response in the expanded samples. Moreover, the expression level of proinflammatory cytokines IL-1, TNF-α, IL-8, and IL-6 were determined by
immunohistochemistry. Immune positivity was detected using secondary antibody
conjugated with horseradish peroxidase (HRP) after staining with diaminobenzidine
tetrahydrochloride (DAB). Results: Histological analysis showed no inflammatory
response was present in the expanded tissue. There was no significant difference in
cytokine production level between the expanded and control samples (p =0.071).
iv
However, there was very strong expression of IL-1 in the expanded tissue as 83 % of
the cells were immune-positive compared to the controls. In contrast, there was no
significant difference in IL-6, IL-8, and TNF-α production. A negative non-significant
correlation between IL-1 immune-positive cells and the inflammatory cells in the
expanded tissue was observed with a Spearman’s correlation coefficient of r= -0.500,
p=0.667. Conclusion: The in vivo experiment revealed a high biocompatibility of the
anisotropic self-inflating hydrogel tissue expander and no significant immunological or
inflammatory reaction were observed between the control and tissue expanded groups. |
|---|