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Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan

A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Avala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum st...

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第一著者: Nurul Amirah , Hassan
フォーマット: 学位論文
出版事項: 2019
主題:
Q Science (General)
QH301 Biology
オンライン･アクセス:http://studentsrepo.um.edu.my/11024/2/Nurul_Amirah.pdf
http://studentsrepo.um.edu.my/11024/1/Nurul_Amirah.pdf
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spelling my.um.stud.110242020-02-21T00:50:10Z Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan Nurul Amirah , Hassan Q Science (General) QH301 Biology A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Avala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (Knuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4–6), temperature (20C–30C), and glucose concentration (10 g/L–50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p < 0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains. 2019-08 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/11024/2/Nurul_Amirah.pdf application/pdf http://studentsrepo.um.edu.my/11024/1/Nurul_Amirah.pdf Nurul Amirah , Hassan (2019) Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/11024/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic Q Science (General)
QH301 Biology
spellingShingle Q Science (General)
QH301 Biology
Nurul Amirah , Hassan
Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan
description A wild-Serbian medicinal mushroom Ganoderma lucidum strain BGF4A1 (GLSB) was isolated from Mount Avala, Serbia and morphologically identified based on its brown-liquorish cap and woody stipe. Molecularly, GLSB (642 bp) was sequenced and found to be 99% similar to the Serbian-originated G. lucidum strain BEOFB 434 and G. lucidum strain BEOFB 431. The isolate belongs to the G. lucidum species as the sequence dissimilarities (Knuc) value between both sequences of the same fungal species was 0.001. In submerged-liquid fermentation, biomass and exopolysaccharide (EPS) production of GLSB was optimised using response surface methodology. The interactions between three variables: initial pH (4–6), temperature (20C–30C), and glucose concentration (10 g/L–50 g/L) were analysed using a central composite design. An analysis of variance revealed that the model was significant for all parameters investigated (p < 0.05). Temperature and glucose concentration were found to significantly influence mycelial biomass production, whereas for EPS production only glucose concentration had a significant effect. The model for biomass and EPS was validated by implementing the optimised conditions (pH 5.26, 50 g/L glucose, and 30C) and was found to generate the highest biomass (3.12 g/L) and EPS (1.96 g/L). An efficient EPS-biomass production blueprint was thus established using optimised parameters for large-scale cultivation of Serbian G. lucidum strains.
format Thesis
author Nurul Amirah , Hassan
author_facet Nurul Amirah , Hassan
author_sort Nurul Amirah , Hassan
title Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan
title_short Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan
title_full Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan
title_fullStr Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan
title_full_unstemmed Optimisation of biomass and exopolysaccharide production in wild Serbian Ganoderma lucidum strain BGF4A1 using response surface methodology / Nurul Amirah Hassan
title_sort optimisation of biomass and exopolysaccharide production in wild serbian ganoderma lucidum strain bgf4a1 using response surface methodology / nurul amirah hassan
publishDate 2019
url http://studentsrepo.um.edu.my/11024/2/Nurul_Amirah.pdf
http://studentsrepo.um.edu.my/11024/1/Nurul_Amirah.pdf
http://studentsrepo.um.edu.my/11024/
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score 13.251813

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