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Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by pseudomonas putida bet001 isolated from palm oil mill effluent

The biosynthesis and characterization of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. The biosynthesis of mcl-PHA in this newly isolated microorganism follows a growth-associated trend. Mcl-PHA accumul...

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Main Authors: Gumel, A.M., Annuar, M.S.M., Heidelberg, T.
Format: Article
Language:English
Published: Public Library of Science 2012
Subjects:
Q Science (General)
QD Chemistry
Online Access:http://eprints.um.edu.my/9635/1/00013161_103470.pdf
http://eprints.um.edu.my/9635/
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spelling my.um.eprints.96352017-07-04T01:03:13Z http://eprints.um.edu.my/9635/ Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by pseudomonas putida bet001 isolated from palm oil mill effluent Gumel, A.M. Annuar, M.S.M. Heidelberg, T. Q Science (General) QD Chemistry The biosynthesis and characterization of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. The biosynthesis of mcl-PHA in this newly isolated microorganism follows a growth-associated trend. Mcl-PHA accumulation ranging from 49.7 to 68.9% on cell dry weight (CDW) basis were observed when fatty acids ranging from octanoic acid (C8:0) to oleic acid (C18:1) were used as sole carbon and energy source. Molecular weight of the polymer was found to be ranging from 55.7 to 77.7 kDa. Depending on the type of fatty acid used, the 1H NMR and GCMSMS analyses of the chiral polymer showed a composition of even and odd carbon atom chain with monomer length of C4 to C14 with C8 and C10 as the principal monomers. No unsaturated monomer was detected. Thermo-chemical analyses showed the accumulated PHA to be semi-crystalline polymer with good thermal stability, having a thermal degradation temperature (Td) of 264.6 to 318.8 (60.2) oC, melting temperature (Tm) of 43.(60.2) oC, glass transition temperature (Tg) of 21.0 (60.2) oC and apparent melting enthalpy of fusion (DHf) of 100.9 (60.1) J g21. Public Library of Science 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/9635/1/00013161_103470.pdf Gumel, A.M. and Annuar, M.S.M. and Heidelberg, T. (2012) Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by pseudomonas putida bet001 isolated from palm oil mill effluent. PLoS ONE, 7 (9). pp. 1-8. ISSN 1932-6203
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic Q Science (General)
QD Chemistry
spellingShingle Q Science (General)
QD Chemistry
Gumel, A.M.
Annuar, M.S.M.
Heidelberg, T.
Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by pseudomonas putida bet001 isolated from palm oil mill effluent
description The biosynthesis and characterization of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. The biosynthesis of mcl-PHA in this newly isolated microorganism follows a growth-associated trend. Mcl-PHA accumulation ranging from 49.7 to 68.9% on cell dry weight (CDW) basis were observed when fatty acids ranging from octanoic acid (C8:0) to oleic acid (C18:1) were used as sole carbon and energy source. Molecular weight of the polymer was found to be ranging from 55.7 to 77.7 kDa. Depending on the type of fatty acid used, the 1H NMR and GCMSMS analyses of the chiral polymer showed a composition of even and odd carbon atom chain with monomer length of C4 to C14 with C8 and C10 as the principal monomers. No unsaturated monomer was detected. Thermo-chemical analyses showed the accumulated PHA to be semi-crystalline polymer with good thermal stability, having a thermal degradation temperature (Td) of 264.6 to 318.8 (60.2) oC, melting temperature (Tm) of 43.(60.2) oC, glass transition temperature (Tg) of 21.0 (60.2) oC and apparent melting enthalpy of fusion (DHf) of 100.9 (60.1) J g21.
format Article
author Gumel, A.M.
Annuar, M.S.M.
Heidelberg, T.
author_facet Gumel, A.M.
Annuar, M.S.M.
Heidelberg, T.
author_sort Gumel, A.M.
title Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by pseudomonas putida bet001 isolated from palm oil mill effluent
title_short Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by pseudomonas putida bet001 isolated from palm oil mill effluent
title_full Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by pseudomonas putida bet001 isolated from palm oil mill effluent
title_fullStr Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by pseudomonas putida bet001 isolated from palm oil mill effluent
title_full_unstemmed Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by pseudomonas putida bet001 isolated from palm oil mill effluent
title_sort biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by pseudomonas putida bet001 isolated from palm oil mill effluent
publisher Public Library of Science
publishDate 2012
url http://eprints.um.edu.my/9635/1/00013161_103470.pdf
http://eprints.um.edu.my/9635/
_version_ 1643688615639777280
score 13.211869

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