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Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design

Phototrophic hydrogen production by indigenous purple non-sulfur bacteria, Rhodopseudomonas palustris PBUM001 from palm oil mill effluent (POME) was optimized using response surface methodology (RSM). The process parameters studied include inoculum sizes ( v/v), POME concentration ( v/v), light inte...

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Main Authors: Jamil, Z., Mohamad Annuar, M.S., Ibrahim, S., Vikineswary, S.
Format: Article
Language:English
Published: 2009
Subjects:
T Technology (General)
TA Engineering (General). Civil engineering (General)
Online Access:http://eprints.um.edu.my/9433/1/Optimization_of_phototrophic_hydrogen_production_by_Rhodopseudomonas_palustris_PBUM001_via_statistical_experimental_design.pdf
http://eprints.um.edu.my/9433/
http://www.scopus.com/inward/record.url?eid=2-s2.0-68349137950&partnerID=40&md5=d8eae97960ff05500c40d640ea5d5d81 http://www.sciencedirect.com/science/article/pii/S0360319909008441 http://ac.els-cdn.com/S0360319909008441/1-s2.0-S0360319909008441-main.pdf?t
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spelling my.um.eprints.94332017-07-03T09:02:00Z http://eprints.um.edu.my/9433/ Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design Jamil, Z. Mohamad Annuar, M.S. Ibrahim, S. Vikineswary, S. T Technology (General) TA Engineering (General). Civil engineering (General) Phototrophic hydrogen production by indigenous purple non-sulfur bacteria, Rhodopseudomonas palustris PBUM001 from palm oil mill effluent (POME) was optimized using response surface methodology (RSM). The process parameters studied include inoculum sizes ( v/v), POME concentration ( v/v), light intensity (klux), agitation (rpm) and pH. The experimental data on cumulative hydrogen production and COD reduction were fitted into a quadratic polynomial model using response surface regression analysis. The path to optimal process conditions was determined by analyzing response surface three-dimensional surface plot and contour plot. Statistical analysis on experimental data collected following Box-Behnken design showed that 100 (v/v) POME concentration, 10 (v/v) inoculum size, light intensity at 4.0 klux, agitation rate at 250 rpm and pH of 6 were the best conditions. The maximum predicted cumulative hydrogen production and COD reduction obtained under these conditions was 1.05 ml H2/ml POME and 31.71 respectively. Subsequent verification experiments at optimal process values gave the maximum yield of cumulative hydrogen at 0.66 ± 0.07 ml H2/ml POME and COD reduction at 30.54 ± 9.85. © 2009 International Association for Hydrogen Energy. 2009 Article PeerReviewed application/pdf en http://eprints.um.edu.my/9433/1/Optimization_of_phototrophic_hydrogen_production_by_Rhodopseudomonas_palustris_PBUM001_via_statistical_experimental_design.pdf Jamil, Z. and Mohamad Annuar, M.S. and Ibrahim, S. and Vikineswary, S. (2009) Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design. International Journal of Hydrogen Energy, 34 (17). pp. 7502-7512. ISSN 0360-3199 http://www.scopus.com/inward/record.url?eid=2-s2.0-68349137950&partnerID=40&md5=d8eae97960ff05500c40d640ea5d5d81 http://www.sciencedirect.com/science/article/pii/S0360319909008441 http://ac.els-cdn.com/S0360319909008441/1-s2.0-S0360319909008441-main.pdf?t DOI 10.1016/j.ijhydene.2009.05.116
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic T Technology (General)
TA Engineering (General). Civil engineering (General)
spellingShingle T Technology (General)
TA Engineering (General). Civil engineering (General)
Jamil, Z.
Mohamad Annuar, M.S.
Ibrahim, S.
Vikineswary, S.
Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design
description Phototrophic hydrogen production by indigenous purple non-sulfur bacteria, Rhodopseudomonas palustris PBUM001 from palm oil mill effluent (POME) was optimized using response surface methodology (RSM). The process parameters studied include inoculum sizes ( v/v), POME concentration ( v/v), light intensity (klux), agitation (rpm) and pH. The experimental data on cumulative hydrogen production and COD reduction were fitted into a quadratic polynomial model using response surface regression analysis. The path to optimal process conditions was determined by analyzing response surface three-dimensional surface plot and contour plot. Statistical analysis on experimental data collected following Box-Behnken design showed that 100 (v/v) POME concentration, 10 (v/v) inoculum size, light intensity at 4.0 klux, agitation rate at 250 rpm and pH of 6 were the best conditions. The maximum predicted cumulative hydrogen production and COD reduction obtained under these conditions was 1.05 ml H2/ml POME and 31.71 respectively. Subsequent verification experiments at optimal process values gave the maximum yield of cumulative hydrogen at 0.66 ± 0.07 ml H2/ml POME and COD reduction at 30.54 ± 9.85. © 2009 International Association for Hydrogen Energy.
format Article
author Jamil, Z.
Mohamad Annuar, M.S.
Ibrahim, S.
Vikineswary, S.
author_facet Jamil, Z.
Mohamad Annuar, M.S.
Ibrahim, S.
Vikineswary, S.
author_sort Jamil, Z.
title Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design
title_short Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design
title_full Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design
title_fullStr Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design
title_full_unstemmed Optimization of phototrophic hydrogen production by Rhodopseudomonas palustris PBUM001 via statistical experimental design
title_sort optimization of phototrophic hydrogen production by rhodopseudomonas palustris pbum001 via statistical experimental design
publishDate 2009
url http://eprints.um.edu.my/9433/1/Optimization_of_phototrophic_hydrogen_production_by_Rhodopseudomonas_palustris_PBUM001_via_statistical_experimental_design.pdf
http://eprints.um.edu.my/9433/
http://www.scopus.com/inward/record.url?eid=2-s2.0-68349137950&partnerID=40&md5=d8eae97960ff05500c40d640ea5d5d81 http://www.sciencedirect.com/science/article/pii/S0360319909008441 http://ac.els-cdn.com/S0360319909008441/1-s2.0-S0360319909008441-main.pdf?t
_version_ 1643688561975754752
score 13.211869

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