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The efficacy of molecular markers analysis with integration of sensory methods in detection of aroma in rice

Allele Specific Amplification with four primers (External Antisense Primer, External Sense Primer, Internal Nonfragrant Sense Primer, and Internal Fragrant Antisense Primer) and sensory evaluation with leaves and grains were executed to identify aromatic rice genotypes and their F1 individuals deriv...

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Main Authors: Yeap, H.Y., Faruq, G., Zakaria, H.P., Harikrishna, J.A.
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2013
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/8582/1/4.pdf
http://eprints.um.edu.my/8582/
http://www.ncbi.nlm.nih.gov/pubmed/24222741
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spelling my.um.eprints.85822019-01-16T01:21:09Z http://eprints.um.edu.my/8582/ The efficacy of molecular markers analysis with integration of sensory methods in detection of aroma in rice Yeap, H.Y. Faruq, G. Zakaria, H.P. Harikrishna, J.A. R Medicine Allele Specific Amplification with four primers (External Antisense Primer, External Sense Primer, Internal Nonfragrant Sense Primer, and Internal Fragrant Antisense Primer) and sensory evaluation with leaves and grains were executed to identify aromatic rice genotypes and their F1 individuals derived from different crosses of 2 Malaysian varieties with 4 popular land races and 3 advance lines. Homozygous aromatic (fgr/fgr) F1 individuals demonstrated better aroma scores compared to both heterozygous nonaromatic (FGR/fgr) and homozygous nonaromatic (FGR/FGR) individuals, while, some F1 individuals expressed aroma in both leaf and grain aromatic tests without possessing the fgr allele. Genotypic analysis of F1 individuals for the fgr gene represented homozygous aromatic, heterozygous nonaromatic and homozygous nonaromatic genotypes in the ratio 20 : 19 : 3. Genotypic and phenotypic analysis revealed that aroma in F1 individuals was successfully inherited from the parents, but either molecular analysis or sensory evaluation alone could not determine aromatic condition completely. The integration of molecular analysis with sensory methods was observed as rapid and reliable for the screening of aromatic genotypes because molecular analysis could distinguish aromatic homozygous, nonaromatic homozygous and nonaromatic heterozygous individuals, whilst the sensory method facilitated the evaluation of aroma emitted from leaf and grain during flowering to maturity stages. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://eprints.um.edu.my/8582/1/4.pdf Yeap, H.Y. and Faruq, G. and Zakaria, H.P. and Harikrishna, J.A. (2013) The efficacy of molecular markers analysis with integration of sensory methods in detection of aroma in rice. Scientific World Journal. ISSN 2356-6140 http://www.ncbi.nlm.nih.gov/pubmed/24222741 PMID: 24222741
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Yeap, H.Y.
Faruq, G.
Zakaria, H.P.
Harikrishna, J.A.
The efficacy of molecular markers analysis with integration of sensory methods in detection of aroma in rice
description Allele Specific Amplification with four primers (External Antisense Primer, External Sense Primer, Internal Nonfragrant Sense Primer, and Internal Fragrant Antisense Primer) and sensory evaluation with leaves and grains were executed to identify aromatic rice genotypes and their F1 individuals derived from different crosses of 2 Malaysian varieties with 4 popular land races and 3 advance lines. Homozygous aromatic (fgr/fgr) F1 individuals demonstrated better aroma scores compared to both heterozygous nonaromatic (FGR/fgr) and homozygous nonaromatic (FGR/FGR) individuals, while, some F1 individuals expressed aroma in both leaf and grain aromatic tests without possessing the fgr allele. Genotypic analysis of F1 individuals for the fgr gene represented homozygous aromatic, heterozygous nonaromatic and homozygous nonaromatic genotypes in the ratio 20 : 19 : 3. Genotypic and phenotypic analysis revealed that aroma in F1 individuals was successfully inherited from the parents, but either molecular analysis or sensory evaluation alone could not determine aromatic condition completely. The integration of molecular analysis with sensory methods was observed as rapid and reliable for the screening of aromatic genotypes because molecular analysis could distinguish aromatic homozygous, nonaromatic homozygous and nonaromatic heterozygous individuals, whilst the sensory method facilitated the evaluation of aroma emitted from leaf and grain during flowering to maturity stages.
format Article
author Yeap, H.Y.
Faruq, G.
Zakaria, H.P.
Harikrishna, J.A.
author_facet Yeap, H.Y.
Faruq, G.
Zakaria, H.P.
Harikrishna, J.A.
author_sort Yeap, H.Y.
title The efficacy of molecular markers analysis with integration of sensory methods in detection of aroma in rice
title_short The efficacy of molecular markers analysis with integration of sensory methods in detection of aroma in rice
title_full The efficacy of molecular markers analysis with integration of sensory methods in detection of aroma in rice
title_fullStr The efficacy of molecular markers analysis with integration of sensory methods in detection of aroma in rice
title_full_unstemmed The efficacy of molecular markers analysis with integration of sensory methods in detection of aroma in rice
title_sort efficacy of molecular markers analysis with integration of sensory methods in detection of aroma in rice
publisher Hindawi Publishing Corporation
publishDate 2013
url http://eprints.um.edu.my/8582/1/4.pdf
http://eprints.um.edu.my/8582/
http://www.ncbi.nlm.nih.gov/pubmed/24222741
_version_ 1643688331775574016
score 13.211869

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