Cover Image

Increasing glucose in ksomaa basal medium on culture day 2 improves in vitro development of cloned caprine blastocysts produced via intraspecies and interspecies somatic cell nuclear transfer

This study was conducted to evaluate the efficiency of potassium simplex optimization medium with amino acids (KSOMaa) as a basal culture medium for caprine intraspecies somatic cell nuclear transfer (SCNT) and caprine-bovine interspecies somatic cell nuclear transfer (iSCNT) embryos. The effect of...

Full description

Saved in:
Bibliographic Details
Main Authors: Abdullah, Ramli, Khadijah, W.E.W., Kwong, P.J.
Format: Article
Published: 2012
Subjects:
QH301 Biology
Online Access:http://eprints.um.edu.my/8430/
http://ac.els-cdn.com/S0093691X12002427/1-s2.0-S0093691X12002427-main.pdf?_tid=ffbd048c-2980-11e3-a7f9-00000aab0f26&acdnat=1380512157_bd69222487da2c01bb96f6bb16196390
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.8430
record_format eprints
spelling my.um.eprints.84302021-04-28T08:07:27Z http://eprints.um.edu.my/8430/ Increasing glucose in ksomaa basal medium on culture day 2 improves in vitro development of cloned caprine blastocysts produced via intraspecies and interspecies somatic cell nuclear transfer Abdullah, Ramli Khadijah, W.E.W. Kwong, P.J. QH301 Biology This study was conducted to evaluate the efficiency of potassium simplex optimization medium with amino acids (KSOMaa) as a basal culture medium for caprine intraspecies somatic cell nuclear transfer (SCNT) and caprine-bovine interspecies somatic cell nuclear transfer (iSCNT) embryos. The effect of increased glucose as an energy substrate for late stage development of cloned caprine embryos in vitro was also evaluated. Enucleated caprine and bovine in vitro matured oocytes at metaphase II were reconstructed with caprine ear skin fibroblast cells for the SCNT and iSCNT studies. The cloned caprine and parthenogenetic embryos were cultured in either KSOMaa with 0.2 mM glucose for 8 days (Treatment 1) or KSOMaa for 2 days followed by KSOMaa with additional glucose at a final concentration of 2.78 mM for the last 6 days (Treatment 2). There were no significant differences in the cleavage rates of SCNT (80.7%) and iSCNT (78.0%) embryos cultured in KSOMaa medium. Both Treatment 1 and Treatment 2 could support in vitro development of SCNT and iSCNT embryos to the blastocyst stage. However, the blastocyst development rate of SCNT embryos was significantly higher (P < 0.05) in Treatment 2 compared to Treatment 1. Increasing glucose for later stage embryo development (8-cell stage onwards) during in vitro culture (IVC) in Treatment 2 also improved both caprine SCNT and iSCNT embryo development to the hatched blastocyst stage. In conclusion, this study shows that cloned caprine embryos derived from SCNT and iSCNT could develop to the blastocyst stage in KSOMaa medium supplemented with additional glucose (2.78 mM, final concentration) and this medium also supported hatching of caprine cloned blastocysts. (C) 2012 Elsevier Inc. All rights reserved. 2012 Article PeerReviewed Abdullah, Ramli and Khadijah, W.E.W. and Kwong, P.J. (2012) Increasing glucose in ksomaa basal medium on culture day 2 improves in vitro development of cloned caprine blastocysts produced via intraspecies and interspecies somatic cell nuclear transfer. Theriogenology, 78 (4). pp. 921-929. ISSN 0093-691X http://ac.els-cdn.com/S0093691X12002427/1-s2.0-S0093691X12002427-main.pdf?_tid=ffbd048c-2980-11e3-a7f9-00000aab0f26&acdnat=1380512157_bd69222487da2c01bb96f6bb16196390 10.1016/j.theriogenology.2012.04.009
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QH301 Biology
spellingShingle QH301 Biology
Abdullah, Ramli
Khadijah, W.E.W.
Kwong, P.J.
Increasing glucose in ksomaa basal medium on culture day 2 improves in vitro development of cloned caprine blastocysts produced via intraspecies and interspecies somatic cell nuclear transfer
description This study was conducted to evaluate the efficiency of potassium simplex optimization medium with amino acids (KSOMaa) as a basal culture medium for caprine intraspecies somatic cell nuclear transfer (SCNT) and caprine-bovine interspecies somatic cell nuclear transfer (iSCNT) embryos. The effect of increased glucose as an energy substrate for late stage development of cloned caprine embryos in vitro was also evaluated. Enucleated caprine and bovine in vitro matured oocytes at metaphase II were reconstructed with caprine ear skin fibroblast cells for the SCNT and iSCNT studies. The cloned caprine and parthenogenetic embryos were cultured in either KSOMaa with 0.2 mM glucose for 8 days (Treatment 1) or KSOMaa for 2 days followed by KSOMaa with additional glucose at a final concentration of 2.78 mM for the last 6 days (Treatment 2). There were no significant differences in the cleavage rates of SCNT (80.7%) and iSCNT (78.0%) embryos cultured in KSOMaa medium. Both Treatment 1 and Treatment 2 could support in vitro development of SCNT and iSCNT embryos to the blastocyst stage. However, the blastocyst development rate of SCNT embryos was significantly higher (P < 0.05) in Treatment 2 compared to Treatment 1. Increasing glucose for later stage embryo development (8-cell stage onwards) during in vitro culture (IVC) in Treatment 2 also improved both caprine SCNT and iSCNT embryo development to the hatched blastocyst stage. In conclusion, this study shows that cloned caprine embryos derived from SCNT and iSCNT could develop to the blastocyst stage in KSOMaa medium supplemented with additional glucose (2.78 mM, final concentration) and this medium also supported hatching of caprine cloned blastocysts. (C) 2012 Elsevier Inc. All rights reserved.
format Article
author Abdullah, Ramli
Khadijah, W.E.W.
Kwong, P.J.
author_facet Abdullah, Ramli
Khadijah, W.E.W.
Kwong, P.J.
author_sort Abdullah, Ramli
title Increasing glucose in ksomaa basal medium on culture day 2 improves in vitro development of cloned caprine blastocysts produced via intraspecies and interspecies somatic cell nuclear transfer
title_short Increasing glucose in ksomaa basal medium on culture day 2 improves in vitro development of cloned caprine blastocysts produced via intraspecies and interspecies somatic cell nuclear transfer
title_full Increasing glucose in ksomaa basal medium on culture day 2 improves in vitro development of cloned caprine blastocysts produced via intraspecies and interspecies somatic cell nuclear transfer
title_fullStr Increasing glucose in ksomaa basal medium on culture day 2 improves in vitro development of cloned caprine blastocysts produced via intraspecies and interspecies somatic cell nuclear transfer
title_full_unstemmed Increasing glucose in ksomaa basal medium on culture day 2 improves in vitro development of cloned caprine blastocysts produced via intraspecies and interspecies somatic cell nuclear transfer
title_sort increasing glucose in ksomaa basal medium on culture day 2 improves in vitro development of cloned caprine blastocysts produced via intraspecies and interspecies somatic cell nuclear transfer
publishDate 2012
url http://eprints.um.edu.my/8430/
http://ac.els-cdn.com/S0093691X12002427/1-s2.0-S0093691X12002427-main.pdf?_tid=ffbd048c-2980-11e3-a7f9-00000aab0f26&acdnat=1380512157_bd69222487da2c01bb96f6bb16196390
_version_ 1698697318831751168
score 13.211869

Similar Items