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Comparison between direct plunging and vitrification techniques on development of mouse embryos at various preimplantation stages

This study evaluated the efficiency of direct plunging and vitrification techniques in preserving mouse embryos at various preimplantation stages. Embryos were from superovulated ICR female mice. In direct plunging, embryos were equilibrated in EG (4.0 M), sucrose (0.25 M), BSA (4%), loaded into str...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Abdullah, Ramli, Khadijah, W.F.W., Bahiyah, A.B.A.
التنسيق: مقال
منشور في: 2012
الموضوعات:
QH301 Biology
الوصول للمادة أونلاين:http://eprints.um.edu.my/8427/
http://www.medwelljournals.com/abstract/?doi=javaa.2012.200.206
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id my.um.eprints.8427
record_format eprints
spelling my.um.eprints.84272021-04-28T08:07:43Z http://eprints.um.edu.my/8427/ Comparison between direct plunging and vitrification techniques on development of mouse embryos at various preimplantation stages Abdullah, Ramli Khadijah, W.F.W. Bahiyah, A.B.A. QH301 Biology This study evaluated the efficiency of direct plunging and vitrification techniques in preserving mouse embryos at various preimplantation stages. Embryos were from superovulated ICR female mice. In direct plunging, embryos were equilibrated in EG (4.0 M), sucrose (0.25 M), BSA (4%), loaded into straw and plunged into LN(2). The straw was thawed in water bath (37 degrees C) and embryos were equilibrated in sucrose (0.5 M), BSA (4%). In vitrification, embryos were equilibrated with EG (7.5%), DMSO (7.5%) with EG (15%), DMSO (15%) and loaded onto cryoleaf. The cryoleaf was plunged into LN(2). Embryos were warned in sucrose (1.0 M), sucrose (0.5 M), washing solution and in modified WM medium. Frozen-thawed/vitrified-warmed embryos were cultured in modified WM medium in 5% CO(2) incubator (37 degrees C). Some embryos were transferred into pseudopregnant CBA/ca females for in vivo viability assessment. Vitrification gave higher survival rates than direct plunging technique at all developmental stages studied. No significant differences in the percentage of live-birth from direct plunging (22.40 +/- 4.40%), vitrification (29.80 +/- 9.00%) and control embryos (28.83 +/- 3.25%). 2012 Article PeerReviewed Abdullah, Ramli and Khadijah, W.F.W. and Bahiyah, A.B.A. (2012) Comparison between direct plunging and vitrification techniques on development of mouse embryos at various preimplantation stages. Journal of Animal and Veterinary Advances, 11 (2). pp. 200-206. ISSN 1680-5593 http://www.medwelljournals.com/abstract/?doi=javaa.2012.200.206
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QH301 Biology
spellingShingle QH301 Biology
Abdullah, Ramli
Khadijah, W.F.W.
Bahiyah, A.B.A.
Comparison between direct plunging and vitrification techniques on development of mouse embryos at various preimplantation stages
description This study evaluated the efficiency of direct plunging and vitrification techniques in preserving mouse embryos at various preimplantation stages. Embryos were from superovulated ICR female mice. In direct plunging, embryos were equilibrated in EG (4.0 M), sucrose (0.25 M), BSA (4%), loaded into straw and plunged into LN(2). The straw was thawed in water bath (37 degrees C) and embryos were equilibrated in sucrose (0.5 M), BSA (4%). In vitrification, embryos were equilibrated with EG (7.5%), DMSO (7.5%) with EG (15%), DMSO (15%) and loaded onto cryoleaf. The cryoleaf was plunged into LN(2). Embryos were warned in sucrose (1.0 M), sucrose (0.5 M), washing solution and in modified WM medium. Frozen-thawed/vitrified-warmed embryos were cultured in modified WM medium in 5% CO(2) incubator (37 degrees C). Some embryos were transferred into pseudopregnant CBA/ca females for in vivo viability assessment. Vitrification gave higher survival rates than direct plunging technique at all developmental stages studied. No significant differences in the percentage of live-birth from direct plunging (22.40 +/- 4.40%), vitrification (29.80 +/- 9.00%) and control embryos (28.83 +/- 3.25%).
format Article
author Abdullah, Ramli
Khadijah, W.F.W.
Bahiyah, A.B.A.
author_facet Abdullah, Ramli
Khadijah, W.F.W.
Bahiyah, A.B.A.
author_sort Abdullah, Ramli
title Comparison between direct plunging and vitrification techniques on development of mouse embryos at various preimplantation stages
title_short Comparison between direct plunging and vitrification techniques on development of mouse embryos at various preimplantation stages
title_full Comparison between direct plunging and vitrification techniques on development of mouse embryos at various preimplantation stages
title_fullStr Comparison between direct plunging and vitrification techniques on development of mouse embryos at various preimplantation stages
title_full_unstemmed Comparison between direct plunging and vitrification techniques on development of mouse embryos at various preimplantation stages
title_sort comparison between direct plunging and vitrification techniques on development of mouse embryos at various preimplantation stages
publishDate 2012
url http://eprints.um.edu.my/8427/
http://www.medwelljournals.com/abstract/?doi=javaa.2012.200.206
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score 13.251813

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