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Effects of oocyte source on the developmental competence of in vitro matured goat oocytes fertilized by the intracytoplasmic sperm injection technique

This study evaluated the effects of oocyte source on in vitro maturation (IVM) and embryo developmental competence in goats by the intracytoplasmic sperm injection (ICSI) technique. Oocytes were obtained either by laparoscopic ovum pick-up (LOPU) or by slicing of abattoir ovaries. The donor does use...

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Main Authors: Abdullah, Ramli, Wan-Khadijah, W.E., Rahman, A.
Format: Article
Published: 2009
Subjects:
QH301 Biology
Online Access:http://eprints.um.edu.my/8399/
http://journals.tubitak.gov.tr/veterinary/issues/vet-09-33-4/vet-33-4-10-0805-1.pdf
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spelling my.um.eprints.83992021-04-28T08:04:32Z http://eprints.um.edu.my/8399/ Effects of oocyte source on the developmental competence of in vitro matured goat oocytes fertilized by the intracytoplasmic sperm injection technique Abdullah, Ramli Wan-Khadijah, W.E. Rahman, A. QH301 Biology This study evaluated the effects of oocyte source on in vitro maturation (IVM) and embryo developmental competence in goats by the intracytoplasmic sperm injection (ICSI) technique. Oocytes were obtained either by laparoscopic ovum pick-up (LOPU) or by slicing of abattoir ovaries. The donor does used for LOPU were superstimulated and their estrus was synchronized. Recovered oocytes from both sources were cultured in microdrops of IVM medium in the presence of CO(2) (5%) at 38.5 degrees C in a CO(2) incubator for 27 h. The ICSI was performed with immobilized sperm on the lid of a culture dish (35 mm) under an Olympus inverted microscope fitted with Narishige micromanipulators. Injected oocytes were cultured for < 192 h in mSOF medium in the CO(2) incubator. Although oocyte recovery per doe was higher (P < 0.05) in abattoir (27.3 +/- 3.0) than LOPU (17.6 +/- 2.5) source, better quality oocytes were obtained from LOPU. Oocyte maturation rate was higher (P < 0.01) in LOPU (73.8 +/- 3.0%) than abattoir-derived (54.0 +/- 0.7%) oocytes. Fertilization and cleavage rates (60.9 +/- 2.1% and 83.1 +/- 2.6%, respectively) were also higher (P < 0.05) with LOPU than abattoir-derived (50.6 +/- 2.3% and 69.3 +/- 4.5%) oocytes. A higher (P < 0.05) proportion of LOPU-derived embryos (20.0 +/- 1.2%) progressed to the morula stage than abattoir (11.3 +/- 4.7%) source. In conclusion, better maturation, fertilization, and embryo development rates were obtained with LOPU-derived oocytes than with the abattoir source. 2009 Article PeerReviewed Abdullah, Ramli and Wan-Khadijah, W.E. and Rahman, A. (2009) Effects of oocyte source on the developmental competence of in vitro matured goat oocytes fertilized by the intracytoplasmic sperm injection technique. Turkish Journal of Veterinary & Animal Sciences, 33 (4). pp. 323-331. ISSN 1300-0128 http://journals.tubitak.gov.tr/veterinary/issues/vet-09-33-4/vet-33-4-10-0805-1.pdf 10.3906/vet-0805-1
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QH301 Biology
spellingShingle QH301 Biology
Abdullah, Ramli
Wan-Khadijah, W.E.
Rahman, A.
Effects of oocyte source on the developmental competence of in vitro matured goat oocytes fertilized by the intracytoplasmic sperm injection technique
description This study evaluated the effects of oocyte source on in vitro maturation (IVM) and embryo developmental competence in goats by the intracytoplasmic sperm injection (ICSI) technique. Oocytes were obtained either by laparoscopic ovum pick-up (LOPU) or by slicing of abattoir ovaries. The donor does used for LOPU were superstimulated and their estrus was synchronized. Recovered oocytes from both sources were cultured in microdrops of IVM medium in the presence of CO(2) (5%) at 38.5 degrees C in a CO(2) incubator for 27 h. The ICSI was performed with immobilized sperm on the lid of a culture dish (35 mm) under an Olympus inverted microscope fitted with Narishige micromanipulators. Injected oocytes were cultured for < 192 h in mSOF medium in the CO(2) incubator. Although oocyte recovery per doe was higher (P < 0.05) in abattoir (27.3 +/- 3.0) than LOPU (17.6 +/- 2.5) source, better quality oocytes were obtained from LOPU. Oocyte maturation rate was higher (P < 0.01) in LOPU (73.8 +/- 3.0%) than abattoir-derived (54.0 +/- 0.7%) oocytes. Fertilization and cleavage rates (60.9 +/- 2.1% and 83.1 +/- 2.6%, respectively) were also higher (P < 0.05) with LOPU than abattoir-derived (50.6 +/- 2.3% and 69.3 +/- 4.5%) oocytes. A higher (P < 0.05) proportion of LOPU-derived embryos (20.0 +/- 1.2%) progressed to the morula stage than abattoir (11.3 +/- 4.7%) source. In conclusion, better maturation, fertilization, and embryo development rates were obtained with LOPU-derived oocytes than with the abattoir source.
format Article
author Abdullah, Ramli
Wan-Khadijah, W.E.
Rahman, A.
author_facet Abdullah, Ramli
Wan-Khadijah, W.E.
Rahman, A.
author_sort Abdullah, Ramli
title Effects of oocyte source on the developmental competence of in vitro matured goat oocytes fertilized by the intracytoplasmic sperm injection technique
title_short Effects of oocyte source on the developmental competence of in vitro matured goat oocytes fertilized by the intracytoplasmic sperm injection technique
title_full Effects of oocyte source on the developmental competence of in vitro matured goat oocytes fertilized by the intracytoplasmic sperm injection technique
title_fullStr Effects of oocyte source on the developmental competence of in vitro matured goat oocytes fertilized by the intracytoplasmic sperm injection technique
title_full_unstemmed Effects of oocyte source on the developmental competence of in vitro matured goat oocytes fertilized by the intracytoplasmic sperm injection technique
title_sort effects of oocyte source on the developmental competence of in vitro matured goat oocytes fertilized by the intracytoplasmic sperm injection technique
publishDate 2009
url http://eprints.um.edu.my/8399/
http://journals.tubitak.gov.tr/veterinary/issues/vet-09-33-4/vet-33-4-10-0805-1.pdf
_version_ 1698697317399396352
score 13.211869

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