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Urinary hyaluronidase activity in rats infected with Blastocystis hominis-evidence for invasion?

The fact whether Blastocystis hominis can invade has always been in question. Apart from a few sporadic studies such as that done on gnotobiotic guinea pigs which showed surface invasion and mucosal inflammation of the host's intestine caused by B. hominis infection, no real documentation of in...

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Main Authors: Chandramathi, S., Suresh, K.G., Mahmood, A.A., Kuppusamy, U.R.
Format: Article
Language:English
Published: 2010
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/8182/1/Chandramathi-2010-Urinary_hyaluronidas.pdf
http://eprints.um.edu.my/8182/
http://link.springer.com/content/pdf/10.1007%2Fs00436-010-1825-y.pdf
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spelling my.um.eprints.81822013-08-02T01:48:33Z http://eprints.um.edu.my/8182/ Urinary hyaluronidase activity in rats infected with Blastocystis hominis-evidence for invasion? Chandramathi, S. Suresh, K.G. Mahmood, A.A. Kuppusamy, U.R. R Medicine The fact whether Blastocystis hominis can invade has always been in question. Apart from a few sporadic studies such as that done on gnotobiotic guinea pigs which showed surface invasion and mucosal inflammation of the host's intestine caused by B. hominis infection, no real documentation of invasion has been proven. Studies have shown that hyaluronidase is secreted during the penetration into the host's skin and gut by nematode parasites. Hyaluronidase activity in protozoa namely Entamoeba histolytica has also been described previously. This study attempts to determine hyaluronidase in urine samples of B. hominis-infected rats. The presence of hyaluronidase in urine provides an indirect evidence of invasion by B. hominis into colonic epithelium causing the degradation of extracellular matrix proteins namely hyaluronic acid (HA). HA is depolymerized by hyaluronidase which may be used by organisms to invade one another. In this study, the levels of urinary hyaluronidase of Sprague-Dawley rats infected with B. hominis were monitored for 30 days. Hyaluronidase levels in the infected rats were significantly higher on days 28 and 30 compared to the day before inoculation (P < 0.01 and P < 0.05, respectively). During this stage, parasitic burden in infected stools was also at a high level. Proinflammatory cytokines, interleukin-6 and interleukin-8, were also significantly higher (P < 0.05) in the serum of infected rats. The study demonstrates that since no other pathogen was present and that amoeboid forms of the parasites have been shown to exist previously, the elevated levels of hyaluronidase in this preliminary finding suggests that the organism is capable of having invasion or penetration activity in the hosts' intestine. 2010 Article PeerReviewed application/pdf en http://eprints.um.edu.my/8182/1/Chandramathi-2010-Urinary_hyaluronidas.pdf Chandramathi, S. and Suresh, K.G. and Mahmood, A.A. and Kuppusamy, U.R. (2010) Urinary hyaluronidase activity in rats infected with Blastocystis hominis-evidence for invasion? Parasitology Research, 106 (6). pp. 1459-1463. ISSN 0932-0113 http://link.springer.com/content/pdf/10.1007%2Fs00436-010-1825-y.pdf 10.1007/s00436-010-1825-y
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Chandramathi, S.
Suresh, K.G.
Mahmood, A.A.
Kuppusamy, U.R.
Urinary hyaluronidase activity in rats infected with Blastocystis hominis-evidence for invasion?
description The fact whether Blastocystis hominis can invade has always been in question. Apart from a few sporadic studies such as that done on gnotobiotic guinea pigs which showed surface invasion and mucosal inflammation of the host's intestine caused by B. hominis infection, no real documentation of invasion has been proven. Studies have shown that hyaluronidase is secreted during the penetration into the host's skin and gut by nematode parasites. Hyaluronidase activity in protozoa namely Entamoeba histolytica has also been described previously. This study attempts to determine hyaluronidase in urine samples of B. hominis-infected rats. The presence of hyaluronidase in urine provides an indirect evidence of invasion by B. hominis into colonic epithelium causing the degradation of extracellular matrix proteins namely hyaluronic acid (HA). HA is depolymerized by hyaluronidase which may be used by organisms to invade one another. In this study, the levels of urinary hyaluronidase of Sprague-Dawley rats infected with B. hominis were monitored for 30 days. Hyaluronidase levels in the infected rats were significantly higher on days 28 and 30 compared to the day before inoculation (P < 0.01 and P < 0.05, respectively). During this stage, parasitic burden in infected stools was also at a high level. Proinflammatory cytokines, interleukin-6 and interleukin-8, were also significantly higher (P < 0.05) in the serum of infected rats. The study demonstrates that since no other pathogen was present and that amoeboid forms of the parasites have been shown to exist previously, the elevated levels of hyaluronidase in this preliminary finding suggests that the organism is capable of having invasion or penetration activity in the hosts' intestine.
format Article
author Chandramathi, S.
Suresh, K.G.
Mahmood, A.A.
Kuppusamy, U.R.
author_facet Chandramathi, S.
Suresh, K.G.
Mahmood, A.A.
Kuppusamy, U.R.
author_sort Chandramathi, S.
title Urinary hyaluronidase activity in rats infected with Blastocystis hominis-evidence for invasion?
title_short Urinary hyaluronidase activity in rats infected with Blastocystis hominis-evidence for invasion?
title_full Urinary hyaluronidase activity in rats infected with Blastocystis hominis-evidence for invasion?
title_fullStr Urinary hyaluronidase activity in rats infected with Blastocystis hominis-evidence for invasion?
title_full_unstemmed Urinary hyaluronidase activity in rats infected with Blastocystis hominis-evidence for invasion?
title_sort urinary hyaluronidase activity in rats infected with blastocystis hominis-evidence for invasion?
publishDate 2010
url http://eprints.um.edu.my/8182/1/Chandramathi-2010-Urinary_hyaluronidas.pdf
http://eprints.um.edu.my/8182/
http://link.springer.com/content/pdf/10.1007%2Fs00436-010-1825-y.pdf
_version_ 1643688238492155904
score 13.211869

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