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Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines

Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extrac...

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Main Authors: Alabsi, A.M., Ali, R., Ali, A.M., Al-Dubai, S.A.R., Harun, H., Abu Kasim, N.H., Alsalahi, A.
Format: Article
Language:English
Published: 2012
Subjects:
RK Dentistry
Online Access:http://eprints.um.edu.my/7731/1/apoptosis_induction.pdf
http://eprints.um.edu.my/7731/
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spelling my.um.eprints.77312013-07-16T03:28:51Z http://eprints.um.edu.my/7731/ Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines Alabsi, A.M. Ali, R. Ali, A.M. Al-Dubai, S.A.R. Harun, H. Abu Kasim, N.H. Alsalahi, A. RK Dentistry Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extracted from Goniothalamus macrophyllus, was investigated for cytotoxic properties against cervical cancer (HeLa), breast carcinoma (MCF-7) and colon cancer (HT29) cells as well as normal mouse fibroblast (3T3) using MTT assay. Fluorescence microscopy showed that GTN is able to induce apoptosis in HeLa cells in a time dependent manner. Flow cytometry further revealed HeLa cells treated with GTN to be arrested in the S phase. Phosphatidyl serine properties present during apoptosis enable early detection of the apoptosis in the cells. Using annexin V/PI double staining it could be shown that GTN induces early apoptosis on HeLa cells after 24, 48 and 72 h. It could be concluded that goniothalamin showing a promising cytotoxicity effect against several cancer cell lines including cervical cancer cells (HeLa) with apoptosis as the mode of cell death induced on HeLa cells by Goniothalamin was. 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/7731/1/apoptosis_induction.pdf Alabsi, A.M. and Ali, R. and Ali, A.M. and Al-Dubai, S.A.R. and Harun, H. and Abu Kasim, N.H. and Alsalahi, A. (2012) Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines. Asian Pacific Journal of Cancer Prevention, 13 (10). pp. 5131-5136. ISSN 1513-7368 10.7314/apjcp.2012.13.10.5131
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic RK Dentistry
spellingShingle RK Dentistry
Alabsi, A.M.
Ali, R.
Ali, A.M.
Al-Dubai, S.A.R.
Harun, H.
Abu Kasim, N.H.
Alsalahi, A.
Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
description Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extracted from Goniothalamus macrophyllus, was investigated for cytotoxic properties against cervical cancer (HeLa), breast carcinoma (MCF-7) and colon cancer (HT29) cells as well as normal mouse fibroblast (3T3) using MTT assay. Fluorescence microscopy showed that GTN is able to induce apoptosis in HeLa cells in a time dependent manner. Flow cytometry further revealed HeLa cells treated with GTN to be arrested in the S phase. Phosphatidyl serine properties present during apoptosis enable early detection of the apoptosis in the cells. Using annexin V/PI double staining it could be shown that GTN induces early apoptosis on HeLa cells after 24, 48 and 72 h. It could be concluded that goniothalamin showing a promising cytotoxicity effect against several cancer cell lines including cervical cancer cells (HeLa) with apoptosis as the mode of cell death induced on HeLa cells by Goniothalamin was.
format Article
author Alabsi, A.M.
Ali, R.
Ali, A.M.
Al-Dubai, S.A.R.
Harun, H.
Abu Kasim, N.H.
Alsalahi, A.
author_facet Alabsi, A.M.
Ali, R.
Ali, A.M.
Al-Dubai, S.A.R.
Harun, H.
Abu Kasim, N.H.
Alsalahi, A.
author_sort Alabsi, A.M.
title Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
title_short Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
title_full Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
title_fullStr Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
title_full_unstemmed Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
title_sort apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines
publishDate 2012
url http://eprints.um.edu.my/7731/1/apoptosis_induction.pdf
http://eprints.um.edu.my/7731/
_version_ 1643688114254774272
score 13.211869

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