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A re-evaluation of plasma sialic acid determination using the periodate resorcinol method versus the enzymatic method

The concentration of plasma sialic acid was estimated using the modified chemical method and the more sensitive enzymatic method in 20 subjects with impaired glucose tolerance and 20 control subjects. The mean sialic acid concentration values of the control subjects and subjects with impaired glucos...

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Main Authors: Muniandy, S., Qvist, R., Zaini, A., Chinna, K., Ismail, I.S.
Format: Article
Language:English
Published: SEAMEO Regional Tropical Medicine and Public Health Network 2005
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/7226/1/A_re-evaluation_of_plasma_sialic_acid_determination.pdf
http://eprints.um.edu.my/7226/
https://www.tm.mahidol.ac.th/seameo/2005_36_4/31-3459.pdf
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spelling my.um.eprints.72262021-04-12T04:44:06Z http://eprints.um.edu.my/7226/ A re-evaluation of plasma sialic acid determination using the periodate resorcinol method versus the enzymatic method Muniandy, S. Qvist, R. Zaini, A. Chinna, K. Ismail, I.S. R Medicine The concentration of plasma sialic acid was estimated using the modified chemical method and the more sensitive enzymatic method in 20 subjects with impaired glucose tolerance and 20 control subjects. The mean sialic acid concentration values of the control subjects and subjects with impaired glucose tolerance using the enzymatic method were 1.747 ± 0.047 and 2.583 ± 0.070 mmole/l and 1.753 ± 0.067 and 2.591 ± 1.02 mmole/l for the chemical method. The intra-assay coefficient of variation for the control subjects and for the subjects with impaired glucose tolerance were 1.963 and 1.583, respectively, for the enzymatic assay and 2.728 and 2.431, respectively, for the chemical assay. The inter-assay coefficient of variation for the control subjects and for the subjects with impaired glucose tolerance were 2.686 and 2.723 for the enzymatic assay, and 3.819 and 3.95 for the chemical assay. Since the values do not differ significantly, the chemical assay is a cost effective method that can be used in large epidemiological studies. SEAMEO Regional Tropical Medicine and Public Health Network 2005 Article PeerReviewed application/pdf en http://eprints.um.edu.my/7226/1/A_re-evaluation_of_plasma_sialic_acid_determination.pdf Muniandy, S. and Qvist, R. and Zaini, A. and Chinna, K. and Ismail, I.S. (2005) A re-evaluation of plasma sialic acid determination using the periodate resorcinol method versus the enzymatic method. The Southeast Asian Journal of Tropical Medicine and Public Health, 36 (4). pp. 1011-1013. ISSN 0125-1562 https://www.tm.mahidol.ac.th/seameo/2005_36_4/31-3459.pdf
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Muniandy, S.
Qvist, R.
Zaini, A.
Chinna, K.
Ismail, I.S.
A re-evaluation of plasma sialic acid determination using the periodate resorcinol method versus the enzymatic method
description The concentration of plasma sialic acid was estimated using the modified chemical method and the more sensitive enzymatic method in 20 subjects with impaired glucose tolerance and 20 control subjects. The mean sialic acid concentration values of the control subjects and subjects with impaired glucose tolerance using the enzymatic method were 1.747 ± 0.047 and 2.583 ± 0.070 mmole/l and 1.753 ± 0.067 and 2.591 ± 1.02 mmole/l for the chemical method. The intra-assay coefficient of variation for the control subjects and for the subjects with impaired glucose tolerance were 1.963 and 1.583, respectively, for the enzymatic assay and 2.728 and 2.431, respectively, for the chemical assay. The inter-assay coefficient of variation for the control subjects and for the subjects with impaired glucose tolerance were 2.686 and 2.723 for the enzymatic assay, and 3.819 and 3.95 for the chemical assay. Since the values do not differ significantly, the chemical assay is a cost effective method that can be used in large epidemiological studies.
format Article
author Muniandy, S.
Qvist, R.
Zaini, A.
Chinna, K.
Ismail, I.S.
author_facet Muniandy, S.
Qvist, R.
Zaini, A.
Chinna, K.
Ismail, I.S.
author_sort Muniandy, S.
title A re-evaluation of plasma sialic acid determination using the periodate resorcinol method versus the enzymatic method
title_short A re-evaluation of plasma sialic acid determination using the periodate resorcinol method versus the enzymatic method
title_full A re-evaluation of plasma sialic acid determination using the periodate resorcinol method versus the enzymatic method
title_fullStr A re-evaluation of plasma sialic acid determination using the periodate resorcinol method versus the enzymatic method
title_full_unstemmed A re-evaluation of plasma sialic acid determination using the periodate resorcinol method versus the enzymatic method
title_sort re-evaluation of plasma sialic acid determination using the periodate resorcinol method versus the enzymatic method
publisher SEAMEO Regional Tropical Medicine and Public Health Network
publishDate 2005
url http://eprints.um.edu.my/7226/1/A_re-evaluation_of_plasma_sialic_acid_determination.pdf
http://eprints.um.edu.my/7226/
https://www.tm.mahidol.ac.th/seameo/2005_36_4/31-3459.pdf
_version_ 1698697304556437504
score 13.211869

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