Validated high performance liquid chromatographic method for analysis of fenvalerate pesticide in chilies by quechers extraction cleanup and high liquid chromatography
Problem statement: Contamination associated with pesticide use has increased as well, adversely impacting the environment and causing human health risks through residues on food. Approach: This study reports the extraction of Fenvalerate (FE) in chilies using QuEChERS based on High Performance Liqui...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/6266/1/Validated_high_performance_liquid_chromatographic_method_for_analysis_of_Fenvalerate_pesticide_in_chilies.pdf http://eprints.um.edu.my/6266/ |
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| Summary: | Problem statement: Contamination associated with pesticide use has increased as well, adversely impacting the environment and causing human health risks through residues on food. Approach: This study reports the extraction of Fenvalerate (FE) in chilies using QuEChERS based on High Performance Liquid Chromatography (HPLC) describes a simple, fast and inexpensive method. The chillie was extracted with acetonitrile Aliquots were cleaned-up using Solid Phase Etraction (dSPE), a primary-secondary amine carbon black. Reversed-phased HPlc system with PDA detection was used for the separation identification and quantification of all these analyses using acetonitrile methanol-potassium dehydrogenate phosphate (50:40:10) as mobile phase. Results: Limit of detection of 0.01 μg mL was obtained. Calibration curves that constructed for the analyzes spiked into samples followed linear relationships with good correlation coefficients (R2>0.9944). In this method was found to be precise, specific and accurate for detection and analysis of Fenvalerate in chilies. Conclusion: QuEChERS methods are convenient, rugged methods that simplify extract cleanup, reduce material costs and improve sample throughout. Here we demonstrate the effectiveness of QuEChERS sample cleanup using a multiresidue analysis of pesticide on chilies. |
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