Determination of simazine and terbuthylazine in olive oil by high performance liquid chromatography with ultraviolet detector
An effective matrix solid-phase dispersion extraction for the determination of simazine and terbuthylazine in olive oil is described. The proposed methodology is based on partitioning liquid-liquid extraction and low temperature precipitation followed by matrix solid-phase dispersion (MSPD), using a...
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| Main Authors: | , , , |
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| Format: | Article |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/6247/ |
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| Summary: | An effective matrix solid-phase dispersion extraction for the determination of simazine and terbuthylazine in olive oil is described. The proposed methodology is based on partitioning liquid-liquid extraction and low temperature precipitation followed by matrix solid-phase dispersion (MSPD), using aminopropyl as dispersant material with a clean-up performed in the elution step with florisil and graphitized carbon (60:40, w/w). Related important factors influencing the extraction efficiency, such as, type of eluent and its volume, type of adsorbent for clean-up step, amount of dispersant material and the ratio of mobile phase were studied and optimized. The method was validated in terms of accuracy and precision (intra-day and inter-day), using spiked samples at different concentration levels. The recoveries were satisfactory (> 91) and limits of detection and quantification as μg of herbicides/g of olive oil sample were: 0.0127 and 0.0540 μg g-1 for simazine, 0.027 and 0.14 μg g-1 for terbuthylazine, respectively. |
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