Staff View: The detection and characterization of pathogenic Leptospira and the use of OMPs as potential antigens and immunogens.

The detection and characterization of pathogenic Leptospira and the use of OMPs as potential antigens and immunogens.

The detection of leptospires in patient blood in the first week of the disease using PCR provides an early diagnostic tool. PCR using two sets of primers (G1/G2 and B64-I/B64-II) tested with samples seeded with 23 leptospiral strains from pathogenic and non-pathogenic strains was able to amplify lep...

Full description

Saved in:

Bibliographic Details
Main Authors:	Gebriel, A.M., Subramaniam, G., Sekaran, S.D.
Format:	Article
Published:	2006
Subjects:	R Medicine (General)
Online Access:	http://eprints.um.edu.my/623/ http://www.ncbi.nlm.nih.gov/pubmed/17322822
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.um.eprints.623
record_format	eprints
spelling	my.um.eprints.6232014-10-21T04:50:04Z http://eprints.um.edu.my/623/ The detection and characterization of pathogenic Leptospira and the use of OMPs as potential antigens and immunogens. Gebriel, A.M. Subramaniam, G. Sekaran, S.D. R Medicine (General) The detection of leptospires in patient blood in the first week of the disease using PCR provides an early diagnostic tool. PCR using two sets of primers (G1/G2 and B64-I/B64-II) tested with samples seeded with 23 leptospiral strains from pathogenic and non-pathogenic strains was able to amplify leptospiral DNA from pathogenic strains only. Of the 39 antibody negative samples collected from patients suspected for leptospirosis, only 1 sample (2.6%) was PCR positive. Using LSSP-PCR, the G2 primers allowed the characterization of Leptopira species to 10 different genetic signatures which may have epidemiological value in determining species involved in outbreaks. Leptospiral outer membrane proteins from three strains were purified and reacted against patients sera and gave rise to different profiles for pathogenic and non-pathogenic strains. Lymphocytes of mice injected with OMPs proliferated and released IFN(-3) when stimulated in vitro using Leptospira OMP as antigens. This suggests that an immune response could be established using leptospiral OMPs as a putative vaccine. OMPs were also used in a Dot-ELISA to detect antibodies against Leptospira pathogens in humans. 2006-12 Article PeerReviewed Gebriel, A.M. and Subramaniam, G. and Sekaran, S.D. (2006) The detection and characterization of pathogenic Leptospira and the use of OMPs as potential antigens and immunogens. Tropical Biomedicine, 23 (2). pp. 194-207. ISSN 0127-5720 http://www.ncbi.nlm.nih.gov/pubmed/17322822 17322822
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	R Medicine (General)
spellingShingle	R Medicine (General) Gebriel, A.M. Subramaniam, G. Sekaran, S.D. The detection and characterization of pathogenic Leptospira and the use of OMPs as potential antigens and immunogens.
description	The detection of leptospires in patient blood in the first week of the disease using PCR provides an early diagnostic tool. PCR using two sets of primers (G1/G2 and B64-I/B64-II) tested with samples seeded with 23 leptospiral strains from pathogenic and non-pathogenic strains was able to amplify leptospiral DNA from pathogenic strains only. Of the 39 antibody negative samples collected from patients suspected for leptospirosis, only 1 sample (2.6%) was PCR positive. Using LSSP-PCR, the G2 primers allowed the characterization of Leptopira species to 10 different genetic signatures which may have epidemiological value in determining species involved in outbreaks. Leptospiral outer membrane proteins from three strains were purified and reacted against patients sera and gave rise to different profiles for pathogenic and non-pathogenic strains. Lymphocytes of mice injected with OMPs proliferated and released IFN(-3) when stimulated in vitro using Leptospira OMP as antigens. This suggests that an immune response could be established using leptospiral OMPs as a putative vaccine. OMPs were also used in a Dot-ELISA to detect antibodies against Leptospira pathogens in humans.
format	Article
author	Gebriel, A.M. Subramaniam, G. Sekaran, S.D.
author_facet	Gebriel, A.M. Subramaniam, G. Sekaran, S.D.
author_sort	Gebriel, A.M.
title	The detection and characterization of pathogenic Leptospira and the use of OMPs as potential antigens and immunogens.
title_short	The detection and characterization of pathogenic Leptospira and the use of OMPs as potential antigens and immunogens.
title_full	The detection and characterization of pathogenic Leptospira and the use of OMPs as potential antigens and immunogens.
title_fullStr	The detection and characterization of pathogenic Leptospira and the use of OMPs as potential antigens and immunogens.
title_full_unstemmed	The detection and characterization of pathogenic Leptospira and the use of OMPs as potential antigens and immunogens.
title_sort	detection and characterization of pathogenic leptospira and the use of omps as potential antigens and immunogens.
publishDate	2006
url	http://eprints.um.edu.my/623/ http://www.ncbi.nlm.nih.gov/pubmed/17322822
_version_	1643686595486810112
score	13.211869

The detection and characterization of pathogenic Leptospira and the use of OMPs as potential antigens and immunogens.

Similar Items