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Fluorescent amplified fragment length polymorphism and pulsed-field gel electrophoresis analyses of multidrug resistant salmonella enterica serotype typhi from different geographical endemic regions in Asia

Sixty-three isolates of Salmonella enterica serotype Typhi (S.typhi) from sporadic cases of typhoid fever obtained from Malaysia (n=6), Vietnam (n=13), India (n=8) and Pakistan (n=36) were characterized by phage typing, drug-susceptibility testing, amplified fragment length polymorphism (AFLP) and p...

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Main Authors: Thong, Kwai Lin, Elgar, Sleman, Bhutta, Zulfikar, Yasin, Rohani, Schreiber, Edgar
Format: Article
Language:English
Published: Malaysian Society for Molecular Biology and Biotechnology 2003
Subjects:
Q Science (General)
QH Natural history
QR Microbiology
Online Access:http://eprints.um.edu.my/5520/1/Fluorescent_amplified_fragment_length_polymorphism_and_pulsed-field_gel_electrophoresis_analyses_of_multidrug_resistant_Salmonella_enterica_serotype_Typhi_from_different_geograph.pdf
http://eprints.um.edu.my/5520/
http://www.msmbb.my/index.php/publication
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spelling my.um.eprints.55202018-10-15T01:18:14Z http://eprints.um.edu.my/5520/ Fluorescent amplified fragment length polymorphism and pulsed-field gel electrophoresis analyses of multidrug resistant salmonella enterica serotype typhi from different geographical endemic regions in Asia Thong, Kwai Lin Elgar, Sleman Bhutta, Zulfikar Yasin, Rohani Schreiber, Edgar Q Science (General) QH Natural history QR Microbiology Sixty-three isolates of Salmonella enterica serotype Typhi (S.typhi) from sporadic cases of typhoid fever obtained from Malaysia (n=6), Vietnam (n=13), India (n=8) and Pakistan (n=36) were characterized by phage typing, drug-susceptibility testing, amplified fragment length polymorphism (AFLP) and pulsed-field gel electrophoresis (PFGE) with restriction endonuclease, XbaI. The strains analyzed were mostly resistant to ampicillin, co-trimoxazole, tetracycline and chloramphenicol. These strains were represented by phage types, E1, E9, 46, J1, VNS and UVS. PFGE analysis showed that multidrug resistant (MDR) strains isolated in different countries and at different periods were genetically very homogenous where 90 of the strains analyzed had very closely related XbaI patterns. AFLP was able to subtype 42 clonally related MDR strains (represented by 8 PFGE patterns) into 16 profiles. Cluster analysis based on the AFLP and PFGE data using unweighted pair group mean averages could differentiate MDR strains from different geographical region and that the drug-sensitive strains were in a distinct cluster. The clustering of MDR strains from each country and the presence of a dominant XbaI-PFGE pattern indicated that the MDR S.typhi had probably been spread clonally in these countries. AFLP is clearly more discriminative than PFGE in differentiating the MDR S.typhi, hence providing an alternative, sensitive method for detailed analysis of the multidrug resistant strains. Malaysian Society for Molecular Biology and Biotechnology 2003 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5520/1/Fluorescent_amplified_fragment_length_polymorphism_and_pulsed-field_gel_electrophoresis_analyses_of_multidrug_resistant_Salmonella_enterica_serotype_Typhi_from_different_geograph.pdf Thong, Kwai Lin and Elgar, Sleman and Bhutta, Zulfikar and Yasin, Rohani and Schreiber, Edgar (2003) Fluorescent amplified fragment length polymorphism and pulsed-field gel electrophoresis analyses of multidrug resistant salmonella enterica serotype typhi from different geographical endemic regions in Asia. Asia Pacific Journal of Molecular Biology and Biotechnology, 11 (1). pp. 43-50. ISSN 0128-7451 http://www.msmbb.my/index.php/publication
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic Q Science (General)
QH Natural history
QR Microbiology
spellingShingle Q Science (General)
QH Natural history
QR Microbiology
Thong, Kwai Lin
Elgar, Sleman
Bhutta, Zulfikar
Yasin, Rohani
Schreiber, Edgar
Fluorescent amplified fragment length polymorphism and pulsed-field gel electrophoresis analyses of multidrug resistant salmonella enterica serotype typhi from different geographical endemic regions in Asia
description Sixty-three isolates of Salmonella enterica serotype Typhi (S.typhi) from sporadic cases of typhoid fever obtained from Malaysia (n=6), Vietnam (n=13), India (n=8) and Pakistan (n=36) were characterized by phage typing, drug-susceptibility testing, amplified fragment length polymorphism (AFLP) and pulsed-field gel electrophoresis (PFGE) with restriction endonuclease, XbaI. The strains analyzed were mostly resistant to ampicillin, co-trimoxazole, tetracycline and chloramphenicol. These strains were represented by phage types, E1, E9, 46, J1, VNS and UVS. PFGE analysis showed that multidrug resistant (MDR) strains isolated in different countries and at different periods were genetically very homogenous where 90 of the strains analyzed had very closely related XbaI patterns. AFLP was able to subtype 42 clonally related MDR strains (represented by 8 PFGE patterns) into 16 profiles. Cluster analysis based on the AFLP and PFGE data using unweighted pair group mean averages could differentiate MDR strains from different geographical region and that the drug-sensitive strains were in a distinct cluster. The clustering of MDR strains from each country and the presence of a dominant XbaI-PFGE pattern indicated that the MDR S.typhi had probably been spread clonally in these countries. AFLP is clearly more discriminative than PFGE in differentiating the MDR S.typhi, hence providing an alternative, sensitive method for detailed analysis of the multidrug resistant strains.
format Article
author Thong, Kwai Lin
Elgar, Sleman
Bhutta, Zulfikar
Yasin, Rohani
Schreiber, Edgar
author_facet Thong, Kwai Lin
Elgar, Sleman
Bhutta, Zulfikar
Yasin, Rohani
Schreiber, Edgar
author_sort Thong, Kwai Lin
title Fluorescent amplified fragment length polymorphism and pulsed-field gel electrophoresis analyses of multidrug resistant salmonella enterica serotype typhi from different geographical endemic regions in Asia
title_short Fluorescent amplified fragment length polymorphism and pulsed-field gel electrophoresis analyses of multidrug resistant salmonella enterica serotype typhi from different geographical endemic regions in Asia
title_full Fluorescent amplified fragment length polymorphism and pulsed-field gel electrophoresis analyses of multidrug resistant salmonella enterica serotype typhi from different geographical endemic regions in Asia
title_fullStr Fluorescent amplified fragment length polymorphism and pulsed-field gel electrophoresis analyses of multidrug resistant salmonella enterica serotype typhi from different geographical endemic regions in Asia
title_full_unstemmed Fluorescent amplified fragment length polymorphism and pulsed-field gel electrophoresis analyses of multidrug resistant salmonella enterica serotype typhi from different geographical endemic regions in Asia
title_sort fluorescent amplified fragment length polymorphism and pulsed-field gel electrophoresis analyses of multidrug resistant salmonella enterica serotype typhi from different geographical endemic regions in asia
publisher Malaysian Society for Molecular Biology and Biotechnology
publishDate 2003
url http://eprints.um.edu.my/5520/1/Fluorescent_amplified_fragment_length_polymorphism_and_pulsed-field_gel_electrophoresis_analyses_of_multidrug_resistant_Salmonella_enterica_serotype_Typhi_from_different_geograph.pdf
http://eprints.um.edu.my/5520/
http://www.msmbb.my/index.php/publication
_version_ 1643687599504621568
score 13.211869

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